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Citations for New England Biolabs :
1 - 50 of 254 citations for Round Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... for the ligation rounds are added to 96 well plates and their 5’ ends phosphorylated with T4 Polynucleotide Kinase (NEB). After 5’ phosphorylation ...
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bioRxiv - Immunology 2020Quote: ... Immulon 2HB 96-well plates were coated with 50 ng/well of Streptavidin (NEB, #N7021S) in 0.1 M NaHCO3 ...
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bioRxiv - Microbiology 2020Quote: ... Ligations were performed in 96 well-plates with 1U T4 Ligase (NEB) and incubated at room temperate overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... and a second round of PCR (NEB Q5 polymerase ...
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bioRxiv - Genomics 2021Quote: ... into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB) ...
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bioRxiv - Genomics 2021Quote: ... We conducted a first round of Exo5/Exo8 (NEB) treatment at 37 °C overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... E7.5) into 96 well PCR plates containing 2.5μl of methylase reaction buffer (1× M.CviPI Reaction buffer (NEB), 2U M.CviPI (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid assembly reactions were performed in 96-well format using the 2X Gibson assembly master mix (NEB) and transformed into chemically-competent BW25141 cells in 96-well format ...
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bioRxiv - Biophysics 2023Quote: ... Assessment of protein phase separation or aggregation was performed by applying a total volume of 20 µL of the sample to a pretreated (Gallego et al., 2020) 16-well glass-bottom ChamberSLIP slide (Grace, BioLabs). DIC imaging was performed as previously described (Gallego et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... and one round of rRNA depletion (NEBNext rRNA depletion kit, NEB). After ethanol precipitation ...
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bioRxiv - Neuroscience 2019Quote: Single nucleus RRBS were performed by first sorting PROX+/FOS+ and /FOS- neuronal nuclei in 96-well plates in 3 µL of 0.1× CutSmart buffer (New England Biolabs) per well as described in the Flow Cytometry section of the Materials and Methods ...
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bioRxiv - Biochemistry 2021Quote: ... Each well of the 96-well destination plate received the following Golden Gate reaction mixture: 0.5 µL of T7 DNA Ligase (NEB; M0318S), 0.5 µL of AarI (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... OFP positive bulk populations were single-cell cloned in 96-well plates and a total of 12 clones screened for mismatches by T7E1 assay (New England Biolabs). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... were added to each quadrant of the 96-well plate (n = 24 unique adapters) with a ligation mixture of 40 Weiss U T4 ligase (NEB), 1mM ATP (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Ninety-six 12 μl Gibson reactions were performed in 96-well PCR plates using Gibson Assembly Master Mix (NEB E2611L). The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells ...
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bioRxiv - Cancer Biology 2022Quote: ... reactions were performed in 96-well plates using 5ng of cDNA samples using the Luna Universal qPCR Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... of each sample was transferred to and digested in a 96-well plate with the restriction enzyme SbfI-HF (NEB). Custom P1 adapters (Hohenlohe et al ...
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bioRxiv - Biochemistry 2019Quote: ... 400ng of library was amplified for 2 rounds using standard Phusion (NEB) PCR conditions ...
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bioRxiv - Immunology 2022Quote: ... A second round of PCR (25 cycles with the Phusion from NEB) was performed to add the Illumina adapters containing the different indexes ...
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bioRxiv - Genetics 2021Quote: The first round of digestion was done with DpnII (New England Biolabs) at 37°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR round 1 and 2 were performed using Q5-High Fidelity polymerase (NEB) for 6 (FS231 and FS232 ...
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bioRxiv - Microbiology 2022Quote: ... Single-round PCR was performed using Q5 high-fidelity DNA polymerase (NEB; M0491) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... A second round of PCR cycle (25 cycles with the Phusion from NEB) was performed to add the Illumina adapters containing the different indexes ...
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bioRxiv - Genomics 2023Quote: ... Both PCR rounds were performed with Q5-High Fidelity polymerase (New England Biolabs) for 6 and 11 cycles ...
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bioRxiv - Immunology 2024Quote: ... Second round nested-PCR reactions were performed using Phusion proof reading polymerase (NEB). A third round of PCR was performed with HotStar Taq DNA polymerase master mix (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by two rounds of poly(A) selection using oligo(dT) magnetic beads (NEB). Small RNAs shorter than 200 nt were then removed from the poly(A ...
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bioRxiv - Neuroscience 2021Quote: ... Three rounds of nested PCR using Phusion HF 2x Master Mix (New England Biolabs) were used to obtain highly specific amplicons for the UNC13A cryptic ...
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bioRxiv - Molecular Biology 2022Quote: ... First round amplifications were performed using Q5 Hot Start High-Fidelity DNA polymerase (NEB) and primer pairs brxRBD_for1/brxRBD_rev1 (wild-type BREX ...
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bioRxiv - Immunology 2021Quote: ... Two rounds of PCR were performed using Q5 High-Fidelity 2X Master Mix (NEB). For the first round of PCR ...
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bioRxiv - Immunology 2021Quote: ... cDNA was amplified in two rounds of PCR using Q5 high-fidelity polymerase (NEB). Adapters necessary for sequencing on the Illumina platform were introduced with the KAPA HyperPrep kit (Roche) ...
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bioRxiv - Genomics 2022Quote: ... Sequencing adapters were then added with two rounds of PCR with Q5 (NEB #M0492) (GWLP P4-7) ...
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bioRxiv - Molecular Biology 2023Quote: ... the first round of PCR was performed using LongAmp Taq 2X master mix (NEB) and 2 – 3 uL of cDNA (1:5 diluted in water ...
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bioRxiv - Microbiology 2020Quote: ... RNA from interface and aqueous fraction was then subjected to two rounds DNase I (NEB) treatment ...
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bioRxiv - Neuroscience 2023Quote: ... two rounds of PCR were conducted using the high fidelity Q5 Polymerase (New England Biolabs). The first round of PCR amplified the ‘naked’ coding region of the gene (*_110 and *_111 primers) ...
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bioRxiv - Molecular Biology 2023Quote: ... A single round of PCR was then performed using Q5 high fidelity DNA polymerase (NEB) with exon-specific primers appended with Illumina forward adapter sequence ...
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Genomics 2021Quote: ... 96 μM S-adenosylmethionine (NEB) and 200 U of M.CviPI (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... polyadenylated RNA was purified by two rounds of binding to Oligo (dT)25 magnetic beads (NEB) and mRNA was fragmented with RNA fragmentation solution (Ambion ...
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bioRxiv - Genetics 2019Quote: ... Barcodes were added in a second round of PCR using Phusion DNA polymerase (NEB cat: M0531S). Samples were sequenced with 76 base-pair paired-end reads using an Illumina NextSeq (Illumina ...
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bioRxiv - Immunology 2023Quote: Phage DNA was subjected to two rounds of PCR using Q5 High-Fidelity 2X Mastermix (NEB), as previously described (Garrett et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... One round of m6A immunoprecipitation was performed using the EpiMark N6-Methyladenosine Enrichment Kit (NEB #E1610S) and protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We did 12 rounds of high-fidelity PCR amplification (Phusion High-Fidelity DNA Polymerase, NEB, Ipswich, MA) using PCR primers that included one of 12 unique Illumina multiplex read indices (Supplementary Table S2) ...
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bioRxiv - Biochemistry 2022Quote: ... Poly(A) RNA was isolated with two rounds of selection using oligo-(dT)25 beads (NEB, S1419S). 50 ng of poly(A ...
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bioRxiv - Immunology 2022Quote: ... A first round of PCR was performed using 1× Standard-Taq magnesium free reaction buffer pack (NEB) with 2 mM MgCl2 (NEB) ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA was amplified in two rounds using NEBNext Ultra II Q5 HiFi polymerase (New England Biolabs). In PCR 1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Products from the first round PCR were then purified using Monarch DNA Gel Extraction Kit (New England Biolabs) and used as the templates for the second round insert PCR ...
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bioRxiv - Genomics 2020Quote: ... After 2 rounds of SPRI cleanup the libraries were eluted in EB buffer and USER enzyme mix (NEB) was used to digest the second strand cDNA ...
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bioRxiv - Cell Biology 2020Quote: ... To start the first round of ligation 40 μl of ligation mix (9.54 U/μl T4 ligase (NEB) in 1x T4 ligase buffer ...
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bioRxiv - Genomics 2019Quote: ... After 2 rounds of SPRI cleanup the libraries were eluted in EB buffer and USER enzyme mix (NEB) was used to digest the second strand cDNA ...
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bioRxiv - Microbiology 2020Quote: First-round PCR product was subjected to an enzymatic cleanup by adding 1μL Antarctic phosphatase (New England Biolabs), 1μL Exonuclease I (New England Biolabs) ...