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Citations for New England Biolabs :
1 - 50 of 116 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... in a thermal cycler with a 2 × Q5 PCR master mix (New England Biolabs, Ipswich, MA, USA). The following program was used ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR amplification was performed in an Eppendorf 2720 Thermal Cycler with either Q5 High-Fidelity DNA Polymerase (New England Biolabs) or GoTaq DNA polymerases (Progema ...
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bioRxiv - Molecular Biology 2023Quote: ... multiplex qPCR was performed on a Bio-Rad C1000 Touch Thermal Cycler using Hot Start Taq 2× Master Mix (NEB) with HT_Forward and HT_Reverse primers (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... PCR was performed on an Applied Biosystems Thermal Cycler using Q5 High-Fidelity 2X Master Mix (New England Biolabs, M0492S). PCR conditions were as follows ...
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bioRxiv - Genetics 2022Quote: ... 2004) PCR reactions were performed in MiniAmp Plus Thermal Cycler with Q5® High-Fidelity DNA polymerase (2000 U/mL) (NEB Ipswich, Massachusetts) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR was measured using the SYBR green master mix (NEB). Cq was used to calculate the relative expression of the target gene by the 2-ΔΔ Cq method ...
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed using Luna Universal qPCR Master Mix (New England Biolabs) and quantified on a Bio-rad CFX96 Real-time Detection System (Bio-rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Real time reactions were performed using Luna® Universal qPCR Master Mix from NEB. Oligonucleotides were used to detect GFP-VAMP (56 and 57 ...
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bioRxiv - Cell Biology 2021Quote: ... The recovered DNA was analyzed by quantitative real-time PCR using LunaScript qPCR mastermix (NEB) and the ABI StepOnePlus qPCR machine and primers in Table 3 ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time qPCR was performed using the Luna Universal Qpcr Master Mix (New England Biolabs), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR reactions were prepared using the Luna® Universal qPCR Master Mix (NEB). Reactions were run and data analyzed using the QuantStudioTM 7 Flex Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed using Luna® Universal qPCR Master Mix (New England Biolabs, M3003S) on an Applied Biosystems StepOnePlus™ Real-Time PCR system with the following conditions ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative PCR was performed on cDNA with Luna Universal qPCR Mastermix (New England Biolabs) in a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Real time qPCR was performed using the Luna Universal Probe One-Step RT-qPCR Kit (NEB) run on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... real time PCR was performed in triplicate using Luna® Universal qPCR Master Mix (New England Biolabs) with variable amounts of cDNA and 0.5 μM of target-specific primers in a CFX connect instrument (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs, catalog no. M3003S) on a QuantStudio 6 instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... Real-time polymerase chain reaction (PCR) was performed using Luna® Universal qPCR Master Mix (NEB M3003L) and the Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1845097) ...
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bioRxiv - Immunology 2024Quote: ... Transcript expression was analyzed via reverse transcriptase quantitative real-time PCR using Lunaprobe reagent (New England Biolabs) on a Quantstudio3 thermocycler (ABI) ...
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bioRxiv - Immunology 2019Quote: ... Real-time qPCR was performed using the Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA) with amplification on the Biorad CFX96 Real Time PCR Detection system ...
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bioRxiv - Genomics 2020Quote: Quantification of several genes in sequence libraries was performed by real-time PCR using NEBnext PCR Master Mix (NEB) supplemented with SYBR Green (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real time PCR (qRT-PCR) was performed with the Luna Universal qPCR Master Mix (New England Biolabs #M3003) on a CFX Connect instrument (BioRad #1855200) ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real time PCR (qRT-PCR) was performed with the Luna Universal qPCR Master Mix (New England Biolabs #M3003) on a CFX Connect instrument (BioRad #1855200) ...
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bioRxiv - Microbiology 2022Quote: ... and real-time PCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) on an ABI 7500 Fast RT-PCR system (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
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bioRxiv - Genomics 2023Quote: ... We performed qPCR on a StepOneTM Real-Time PCR System with the Luna® Universal qPCR Master Mix (NEB). Gene specific primers are outlined in Extended Data Table S4.
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bioRxiv - Plant Biology 2022Quote: ... were analyzed by Quantitative real time PCR (qRT PCR) using Luna® Universal qPCR Master Mix (New England BioLabs) in a QuantStudio® 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... One-tenth of the RT reaction was used as a template for real-time PCR using Luna Universal qPCR Master Mix (New England Biolabs) on a QuantStudi 6 system ...
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bioRxiv - Plant Biology 2021Quote: ... For real-time qPCR experiments oligo(dT)-primed cDNAs were synthesized using 2ug of total RNA with MMLV (reverse transcriptase, NEB). qRT-PCR reactions were set up with 50-70ng of cDNA ...
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bioRxiv - Immunology 2022Quote: ... integrated HIV DNA and unspliced HIV RNA was performed by modified nested real-time PCR assay using Taq DNA polymerase (BioLabs) in the first PCR and TaqMax Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplifications were performed in an Applied Biosystems 7500 Fast Real-Time PCR System using the Luna Universal Probe Mix (NEB). We evaluated the performance of the qPCR primers and probes using a DNA concentration gradient spanning 0.1-100.0 ng/reaction in single gene reactions as well as in a multiplex reaction ...
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bioRxiv - Microbiology 2020Quote: The quantification of DWV genome copies was performed by SYBR-Green Real-Time Quantitative PCR (qPCR) using Luna Universal qPCR master mix (New England Biolabs), 0.25 μM forward and reverse DWV_qPCR primers and 2 μl of cDNA ...
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bioRxiv - Microbiology 2022Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (NEB) with the primers listed in (Table S3) ...
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bioRxiv - Microbiology 2023Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs) with the primers listed in Table S4 ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 96-well plate and qPCR was performed in a LightCycler 96 System (Roche) ...
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bioRxiv - Immunology 2024Quote: ... Two microliters were subsequently used in real-time quantitative PCR reactions containing SYBR-green based Luna universal dye qPCR mix (New England Biolabs) and gene specific PCR primers ...
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bioRxiv - Molecular Biology 2022Quote: The rest of RNAs were performed with real-time reverse transcription PCR (RT-qPCR) using a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s recommendations by a thermocycler (BIO-RAD CFX96™ Real-Time System) ...
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bioRxiv - Systems Biology 2019Quote: ... Both real-time and first-step PCRs were done similarly to the manufacturer’s protocol for Phusion polymerase (New England BioLabs, Ipswich, MA). For qPCR ...
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bioRxiv - Pathology 2021Quote: ... in an Applied Biosystems StepOnePlus Real-Time PCR System (USA) and tracked with a DNA-intercalant green fluorophore provided with the WarmStart LAMP Kit (NEB, USA). The RT-LAMP amplification was set to 45 minutes for experiments illustrated in Figures 2 ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed to quantify the expression level of mRNAs by Luna® Universal qPCR Master Mix (NEB, M3003E) and LightCycler ®480 Real-Time PCR System (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Quantification is based on real-time SYBR green amplification molecules with specific target primers using Luna® Universal qPCR Master Mix (Biolabs). Relative mRNA expression is calculated using the CFX Manager software (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and quantified by qPCR on a Bio-Rad CFX Connect Real-Time PCR detection system with the NEBNext Library Quant Kit for Illumina (New England Biolabs, catalog # E7630L). The libraries were normalized to 10 nM ...
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bioRxiv - Biochemistry 2021Quote: ... oligonucleotide annealing was carried out in a thermocycler in T4 polynucleotide kinase reaction buffer (NEB) / 50 mM potassium acetate by heating oligonucleotide mixtures to 95 °C for 5 minutes ...
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bioRxiv - Physiology 2023Quote: ... thermocycler with the Luna Universal one-step qPCR Master Mix (E3005; New England Biolabs, Ispwich, MA, USA). Amplification reactions for genes of interest were performed in 50 cycles of the following cycling protocol ...
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Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... PCR mix and thermocycler conditions were set according to the Phusion Master Mix protocol provided by NEB. PCR products were measured and visualized using a D5000 ScreenTape System (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... then quantified with the NEBNext® Library Quant Kit for Illumina® using qPCR on a StepOne Plus real-time PCR platform (New England Biolabs® Inc., MA, USA). Following qPCR ...
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bioRxiv - Microbiology 2023Quote: ... Purified PCR products were incubated together in a thermocycler with 1X Hi-Fi DNA Assembly master mix (NEB) at 50°C for one hour ...
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bioRxiv - Bioengineering 2021Quote: ... Resulting amplicons were denatured and reannealed in a thermocycler before nuclease reaction using T7 Endonuclease 1 (New England Biolabs) at 37C for 20min ...
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bioRxiv - Immunology 2020Quote: ... Amplicon DNA was denatured and reannealed in a thermocycler prior to cleaving by T7 Endonuclease 1 (New England Biolabs) at 37°C in a 30min reaction ...
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bioRxiv - Immunology 2024Quote: ... Template DNA digestion was carried out at 37 °C for 30 minutes in a thermocycler block prior to purification of the RNA using a Monarch RNA Cleanup Kit (New England Biolabs). Purified mRNA was eluted in DEPC-treated water and stored at -80 °C prior to use ...
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bioRxiv - Immunology 2023Quote: ... The PCR thermal conditions were conducted using Q5® High-Fidelity DNA Polymerase (New England Biolabs, USA) at two step-PCR 98 °C 30 sec ...