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Citations for New England Biolabs :
1 - 50 of 89 citations for Rat IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Genomics 2020Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Systems Biology 2019Quote: ... and shrimp alkaline phosphatase (SAP, NEB). The concentrations of the caps ...
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bioRxiv - Genomics 2024Quote: ... followed by dephosphorylation with Quick SAP (NEB). gRNAs were re-folded prior ABE8e:gRNA complexation ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were treated with Shrimp Alkaline Phosphatase (SAP; from NEB). In short ...
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bioRxiv - Genomics 2019Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 µl at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 μl at 37°C for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were processed according to the Exo-SAP protocol (NEB) and Sanger-sequenced by Azenta Life Services GeneWiz with hisC-ins-f as sequencing primer ...
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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bioRxiv - Biochemistry 2020Quote: ... the pTXB1 plasmid was double-digested with Nde-I and Sap-I (New England Biolabs, Ipswich, MA, USA) enzymes at the respective restriction sites to generate binding sites for insert ligation ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified products were cleaned using Exonuclease-Shrimp Alkaline Phosphatase (Exo-SAP) mixture (New England Biolabs, Ipswich, Massachusetts) and sequenced bidirectionally using BigDye v3.1 terminator kit in ABI 3500XL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were run by electrophoresis on a 2% agarose gel to ensure the absence of primer-dimers and PCR samples for each primer pair-opsin target were purified using Exonuclease (EXO) and Shrimp alkaline phosphatase (SAP) (NEB) prior to Sanger Sequencing to confirm gene-specific amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of DNA isolate was used for PCR (primers are in supplementary table 1.4) and PCR cleanup was performed using Exo-SAP (NEB, M0293, M0371). Sanger Sequencing reactions were prepared using (Thermo Fisher # 4337455) ...
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bioRxiv - Plant Biology 2021Quote: ... 500 ng of the SAP-treated RNA was treated with 12.5 units RNA 5’ Pyrophophohydrolase (RppH; New England BioLabs; Ipswitch, MA, USA) in 1X T4 RNA Ligase Buffer (New England BioLabs ...
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bioRxiv - Microbiology 2024Quote: PCR products of Asaia positive samples were cleaned up using Exonuclease I (Exo I) and Shrimp Alkaline Phosphate (Exo-SAP protocol) according to the protocol of New England Biolabs (NEB, MA, and USA) and sent for sequencing ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs), following the manufactures instructions.
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bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs, USA) that specifically depletes cytoplasmic (5S ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and on the Sprague Dawley (SD) strain (rats) (Hera Biolabs) were obtained from vendor and breed in the Division of Laboratory Animal Resources (DLAR ...
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bioRxiv - Microbiology 2023Quote: ... plasmid DNA encoding rat HEV was linearized using EcoRI (NEB). Five percent of the reaction was subjected to gel electrophoresis with ethidium bromide staining and visualized with ultraviolet light to verify that linearization had occurred.
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used were: Alexa-680 goat anti-mouse IgG and Alexa-800 goat anti-rabbit IgG (New England Biolabs). For DRGN cultures ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatants were immunoprecipitated overnight with 40 µL of precoated anti-IgG magnetic beads (goat anti-rabbit IgG magnetic beads, NEB) previously incubated with the antibody of interest for 4 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2021Quote: ... The entire rat Cx43 sequence was removed using EcoRI (Cat. No. R3101S; NEB) and BamHI (Cat ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Immunology 2021Quote: Purified recombinant CV3-25 IgG was mixed with LysC (NEB) at a ratio of 1μg LysC per 10mg of IgG and incubated at 37°C for 18h with nutation ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Cancer Biology 2023Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat; NEB, E6310X). rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... the cell extracts were incubated with IgG-coupled magnetic beads (NEB) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Magnetic beads conjugated with IgG Protein A/G (New England Biolabs) were pre-blocked with 0.5 mg/mL of sonicated salmon sperm DNA (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... or an appropriate isotype-matched control (polyclonal goat isotype IgG, NEB) was added at a final concentration of 10 μg/ml and incubated overnight ...
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bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) according to the manufacturer’s instructions with 6 µl total RNA used as input per sample ...
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bioRxiv - Neuroscience 2020Quote: ... Mutations were generated in the rat P2X2 receptor using the Q5 Site-Directed Mutagenesis Kit (NEB). Each mutation was verified by DNA sequencing and subcloned in pWPT-EF1α-P2X2-GCaMP6s-IRES-DsRed2 or pWPT-EF1α-P2X2-GCaMP6s-P2A-mScarlet lentiviral vectors.
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) or anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... Purified IgG was denatured and treated with PNGase enzyme (New England Biolabs) for 12hours at 37°C to release glycans.
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Microbiology 2020Quote: ... or anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) were used as the secondary antibodies.
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...