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Citations for New England Biolabs :
1 - 50 of 614 citations for Rabbit Anti Human IgG gamma chain Alexa Fluor 350 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used were: Alexa-680 goat anti-mouse IgG and Alexa-800 goat anti-rabbit IgG (New England Biolabs). For DRGN cultures ...
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bioRxiv - Microbiology 2019Quote: ... followed by Alexa Fluor 488 goat anti-rabbit secondary antibody (New England Biolabs, UK) for 1h ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C followed by Alexa Fluor 488 goat anti-rabbit secondary antibody (New England Biolabs, UK) for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatants were immunoprecipitated overnight with 40 µL of precoated anti-IgG magnetic beads (goat anti-rabbit IgG magnetic beads, NEB) previously incubated with the antibody of interest for 4 h at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... or without 350 units of recombinant GSK3β (rabbit skeletal muscle) (New England BioLabs) and 1X hot kinase buffer (50mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... cells were incubated with 2 nM benzylguanine-Alexa Fluor 647 (New England Biolabs, SNAP-Surface Alexa Fluor 647) or custom synthesized SNAP substrate benzylguanine-DY549P1 for 30 min before an experiment ...
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) or anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: Human ARHGAP18 constructs were created using Polymerase Chain Reaction (PCR) using New England Biolabs (NEB) Phusion High-Fidelity PCR Kit (catalog no ...
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bioRxiv - Neuroscience 2023Quote: SNAP-Surface® Alexa Fluor® 488 (NEB) were dissolve in DMSO to yield a labeling stock solution of 1 mM tags’ substrate ...
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bioRxiv - Immunology 2019Quote: ... and SNAP-Surface Alexa Fluor 647 (NEB, catalog S9136S) at 37 °C/ 5% CO2 for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alexa Fluor 647-Phalloidin (8940) was obtained from NEB. Images were taken on an inverted confocal microscope (Leica TCS SP5 ...
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bioRxiv - Biophysics 2022Quote: ... SNAP-Surface Alexa Fluor 647 (S9136S, New England Biolabs), SNAP-Surface ATTO488 (S9124S ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μM of SNAP-Surface Alexa Fluor 647 (NEB) was added and incubated for another 30 min on ice to saturate the remaining SNAP-tag binding sites ...
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bioRxiv - Immunology 2020Quote: ... before being incubated with alkaline phosphatase-labeled anti-rabbit IgG (New England Biolabs, Beverly, MA).
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bioRxiv - Biophysics 2022Quote: ... and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB) in extracellular buffer for 30 minutes at 37 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Tetravalent antibody constructs were generated by fusing these fragments with their respective IgG heavy chain in the pSCSTa mammalian expression vector using Gibson assembly (NEB). Fab-IgG constructs were arranged by fusing a heavy chain Fab domain to the N-terminus of the IgG via a S(G4S)3 linker ...
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bioRxiv - Immunology 2022Quote: ... 5 mol% labelled with SNAP-Surface Alexa Fluor 488 (NEB, S9128S), to 25% v/v HeLa lysate in a final volume of 20 µl (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP-Surface® Alexa Fluor® 647 (New England Biolabs). Protein (5 µM ...
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bioRxiv - Immunology 2022Quote: ... and Illumina linker addition to B cell heavy chain transcripts were performed using the human NEBNext Immune Sequencing Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The fluorescent probe SNAP-Surface Alexa Fluor 546 (#S9132S) was from NEB Biotech ...
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bioRxiv - Biophysics 2024Quote: ... SNAP-Surface Alexa Fluor 647 (catalog no. S9136S) was obtained from NEB. 40 nm and 90 nm diameter gold nanoparticles (catalog no ...
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bioRxiv - Microbiology 2024Quote: ... beads were incubated with 10 μM SNAP-Surface Alexa Fluor 488 (NEB) for 1 hour at 4°C prior to elution ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was labeled with gamma-32P-ATP using T4 PNK (NEB). After washing with PNK buffer ...
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bioRxiv - Genomics 2023Quote: ... 350 U of DpnII 50U/µl (NEB cat. R0543M) in 300 µl 1x NEBuffer DpnII (NEB cat ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (anti-rabbit IgG HRP, 1:5000 dilution, 7074S, NEB) in TBS-T with 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... dCas9-SNAP protein samples were incubated with SNAP-Surface Alexa Fluor 647 (NEB S9136) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: The full-length PLCγ1 was labeled with SNAP-Surface Alexa Fluor 647 (NEB, S9136S). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... purified proteins were labelled with SNAP-Surface Alexa Fluor 647 tag (New England Biolabs) following manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... Anti-rabbit (#7074, NEB) and anti-mouse (#7076 ...
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bioRxiv - Immunology 2021Quote: VH and VL sequences of candidate sequences were cloned into a pcDNA.3 based vector with dual CMV promotor harboring IgG1 heavy chain and light chain backbone using NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Immunology 2023Quote: ... and the V regions were inserted into cut backbone vectors (heavy chain: FJ475055; kappa chain: FJ75056) via Gibson reaction (New England Biolabs). Successful clones were prepared ...
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bioRxiv - Cell Biology 2019Quote: ... The bead-bound protein was incubated with 3 µM SNAP-Surface Alexa Fluor 647 (NEB) for 40-60 min at 4°C (to fluorescently label the protein) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was labeled with SNAP-Surface Alexa Fluor 647 labeling kit (New England Biolabs) according to the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... rabbit anti-DYKDDDDK (#D6W5B; NEB) and rabbit anti-myc (#71D10 ...
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bioRxiv - Synthetic Biology 2022Quote: ... rabbit anti-SNAP-tag (NEB; P9310 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with 5 μM cell-impermeable SNAP-Surface Alexa Fluor 488 (New England Biolabs) in complete media (DMEM + 10% FBS ...
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bioRxiv - Biophysics 2022Quote: Cells used for calcium imaging experiments were labeled using 1 µM SNAP-Surface Alexa Fluor 647 (NEB) and 4 µM Oregon Green 488 BAPTA-1 (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: FLAG-TEV-SNAP-Ago2 expressed in HEK293T cells was labeled with SNAP-Surface Alexa Fluor 647 (NEB) and immunopurified by an anti-FLAG antibody conjugated onto Dynabeads Protein G (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... 5% CO2 -balanced complete cell culture medium with 5 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), for 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Cell Biology 2021Quote: ... FHIP1B or FHIP2A-coated IgG beads were labeled with 5 µM HaloTag-Alexa-660 (New England Biolabs) in the column for 10 minutes at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and rabbit anti-myc (#71D10; NEB) primary antibodies were used ...
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bioRxiv - Immunology 2021Quote: The cDNA sequences of the paired variable heavy and light chain region of anti-RBD antibody clones were synthesized as gBlocks (IDT) and cloned by the Gibson assembly (NEB) into human IgG1 heavy chain and light chain expression plasmids ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence labelling was done by incubating with 5 nmol SNAP-Surface Alexa Fluor 488 (#S9129S, New England Biolabs) for 1 hour at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... The coverslips were incubated in staining solution (1 µM benzylguanine Alexa Fluor 647 (S9136S, NEB, Ipswich, MA, USA); 1 mM DTT ...
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bioRxiv - Immunology 2020Quote: SNAP-ASC-CARD and CASP1-CARD-SNAP monomers that were labeled with Alexa Fluor 647 (New England Biolabs) were co-incubated with CARD domain only filament seeds (CARD8-CARD without SNAP ...
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bioRxiv - Microbiology 2022Quote: ... the template chain was digested using DpnI restriction endonuclease (NEB, USA). Afterward ...
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bioRxiv - Molecular Biology 2020Quote: ... Polymerase chain reactions (PCRs) were performed using Phusion DNA polymerase (NEB) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: Polymerase chain reaction (PCR) was performed with Phusion DNA polymerase (NEB) in a total volume of 50 µl in GC buffer containing 10% (v/v ...