Labshake search
Citations for New England Biolabs :
1 - 50 of 223 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The RT-RPA was performed at 42°C for 30 min by combining M-MuLV-RT (NEB #M0253L) with TwistAmp Basic (TwistDx #TABAS03KIT) ...
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bioRxiv - Biochemistry 2022Quote: ... EvaGreen DNA dye was replaced with RPA fluorescent probes (DENV2-CAP-probe at 0.12 μM) with 100 U Exonuclease III (NEB), keeping all other reagents same ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W. Cai) and 0.03 U/μl T4 DNA polymerase (NEB, #M0203) at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: All reagents from NEB except where noted otherwise ...
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bioRxiv - Synthetic Biology 2022Quote: ... molecular biology reagents from NEB and Life Technologies and general laboratory reagents from Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... using Gibson Assembly reagents (NEB). Select elements were removed from the backbone (such as rtTA3 ...
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bioRxiv - Genetics 2021Quote: ... PCR reagents were purchased from NEB, and all primers were synthesized by Integrated DNA Technologies (IDT).
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bioRxiv - Microbiology 2024Quote: Molecular biology reagents were from NEB and chemical reagents were from Sigma unless otherwise noted ...
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bioRxiv - Molecular Biology 2019Quote: ... and inserted with NEBuilder reagents (New England Biolabs) into pBACc3 opened with BamHI and PstI ...
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bioRxiv - Biochemistry 2020Quote: ... Reagents for the KLD reactions came from NEB: T4 PNK (M0236S) ...
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bioRxiv - Genomics 2020Quote: ... NEBNext DNA Ultra II Reagents (NEB, Ipswich, MA) were used according to the manufacturer’s instructions for end repair ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted using TRI reagent (BioLabs) according to the manufacturer specifications ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext DNA Ultra II Reagents (NEB, Ipswich, MA) were used according to the manufacturer’s instructions for end repair ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reagents for the KLD reactions came from NEB: T4 PNK (Cat #M0236S) ...
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bioRxiv - Molecular Biology 2023Quote: ... NEBNext DNA Ultra II Reagents (NEB, Ipswich, MA) were used according to the manufacturer’s instructions for end repair ...
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bioRxiv - Cell Biology 2021Quote: qRT-PCRs were performed using Luna OneStep reagent (NEB) on biological triplicates ...
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bioRxiv - Biochemistry 2022Quote: ... Cloning reagents were purchased from New England BioLabs (NEB). Codon-optimized gene fragments were purchased from Genewiz ...
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bioRxiv - Synthetic Biology 2020Quote: ... and reagents were purchased from New England Biolabs (NEB) and used according to provided protocols ...
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bioRxiv - Immunology 2020Quote: ... PCR amplification was conducted using Phusion HF reagents (NEB) and employed the following cycling conditions ...
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bioRxiv - Microbiology 2019Quote: ... and Gibson assembly reagents (NEB HiFi Assembly Master Mix) for generation of recombinant DNAs and for routine molecular cloning and subcloning procedures were obtained from New England Biolabs (Ipswitch ...
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bioRxiv - Bioengineering 2022Quote: ... and reagents were sourced from New England Biolabs (NEB) unless otherwise noted ...
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bioRxiv - Microbiology 2022Quote: ... Other standard cloning reagents include T4 DNA ligase (NEB), restriction enzymes (NEB) ...
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bioRxiv - Biochemistry 2023Quote: All reagents were purchased from NEB (New England Biolabs) and DNA oligonucleotides were obtained from IDT (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... All the reagents were from New England Biolabs (NEB) and custom primers were from Integrated DNA Technologies (IDT) ...
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bioRxiv - Biochemistry 2024Quote: ... Cloning reagents were purchased from New England BioLabs (NEB). Codon-optimized gene fragments were purchased from Twist Biosciences ...
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bioRxiv - Developmental Biology 2020Quote: ... and the Uracil-Specific Excision Reagent enzyme (NEB, cat.: M5505L) was used to digest the products.
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bioRxiv - Molecular Biology 2020Quote: ... Gibson Assembly reagent was purchased from New England Biolabs (NEB). Genemorph II Random Mutagenesis kits and QuikChange mutagenesis kits were purchased from Agilent Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... using Gibson Assembly reagent mix (New England Biolabs; Beverley, MA) [69] ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared using NEBNext Ultra library prep reagents (NEB) according to manufacturer”s instructions and sequenced using a 2 × 250-cycle MiSeq Reagent kit v3.0 (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR was performed with Luna® reagent (New England Biolabs) in Roche LightCycler® 480 ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed with Luna Universal qPCR reagent (NEB). eMLV env was detected with 5’-AGGCTGTTCCAGAGATTGTG-3’ and 5’-TTCTGGACCACCACATGAC-3’ and 18S rRNA was detected with 5’-GTAACCCGTTGAACCCCATT-3’ and 5’-CCATCCAATCGGTAGTAGCG-3’ on Roche LightCycler 480 II (104,105).
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bioRxiv - Plant Biology 2021Quote: ... qPCR was performed with Luna® reagent (New England Biolabs). 2 primer pairs detecting both CHC1 and CHC2 transcripts were employed ...
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bioRxiv - Genomics 2022Quote: ... NEBNext® Ultra II DNA Library Prep reagents (NEB #E7645) were used to ligate 5mC-protected adapters ...
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bioRxiv - Bioengineering 2022Quote: ... via Gibson Assembly using reagents from New England Biolabs (NEB). Sub-cloning and other modifications to plasmids were also performed with Gibson Assembly using reagents from NEB ...
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bioRxiv - Microbiology 2023Quote: ... Reagents and enzymes were supplied by New England Biolabs (NEB), Merck and Thermo-Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... reagent following the manufacturer instructions and then treated with DnaseI (NEB). For the RT-qPCRs showed in Figure 2F ...
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bioRxiv - Molecular Biology 2019Quote: ... with Luna Universal One-Step RT-qPCR reagents (New England Biolabs). 50 ng total RNA and 250 nM primers were added in each reaction ...
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bioRxiv - Microbiology 2019Quote: ... The adapter loop was opened by Uracil-Specific Excision Reagent (NEB) to excise the uracil ...
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bioRxiv - Bioengineering 2021Quote: All chemicals were sourced from Sigma-Aldrich and enzymes and reagents for DNA manipulation were purchased from NEB unless otherwise noted ...
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bioRxiv - Bioengineering 2020Quote: ... such that a Uracil-Specific Excision Reagent (USER, New England Biolabs) will cut the cDNA at these locations thereby releasing the desired product and permitting amplification of this product via PCR ...
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bioRxiv - Biochemistry 2022Quote: ... The USER™ (uracil-specific excision reagent) Friendly Cloning Kit (NEB) was used for all the cloning procedures ...
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bioRxiv - Molecular Biology 2019Quote: ... and Illumina libraries were prepared using NEBNext reagents (New England Biolabs). The quality ...
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bioRxiv - Microbiology 2019Quote: ... and Gibson Assembly Reagent were obtained from New England Biolabs (NEB). A comprehensive list of primers ...
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bioRxiv - Synthetic Biology 2020Quote: ... by USER™ (uracil-specific excision reagent) cloning (New England Biolabs), as previously described (D’Ambrosio et al. ...
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bioRxiv - Microbiology 2020Quote: ... or NEBNext reagents (New England Biolabs, Ipswich, MA USA, catalog# E6040) and the resulting libraries were then sequenced on an Illumina HiSeq2000/2500 which generated paired-end reads of 100 nucleotides (nt) ...
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bioRxiv - Immunology 2021Quote: We used NEBNext Ultra II RNA Prep reagents (New England Biolabs) to prepare Illumina-ready libraries ...
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bioRxiv - Microbiology 2020Quote: ... and Gibson Assembly Reagent were acquired from New England Biolabs (NEB). GoTaq Green Master Mix was obtained from Promega ...
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bioRxiv - Microbiology 2023Quote: ... were suspended in 1 × DNA/RNA Protection Reagent (New England Biolabs), and RNA Lysis Buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using LUNA SYBR green reagent (New England Biolabs) on a Qiagen Rotorgene instrument ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... the two inner solutions were comprised of 1) PURExpress reagents (NEB) or 2 ...