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Citations for New England Biolabs :
1 - 50 of 10000+ citations for RNA Amplification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cDNA synthesis and amplification were performed with Next Ultra RNA Library Preparation Kit (NEB). Libraries were sequenced on a HiSeq3000 (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... and PCR amplification were performed using the Truseq Small RNA Sample Prep Kit for Illumina (NewEngland Biolabs, E7335S) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Library amplification: Amplification was performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g ...
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bioRxiv - Genomics 2022Quote: Real-time PCR (RT-PCR) amplification of RNA was performed following the Luna Universal One-Step RT-qPCR kit (New England Biolabs). A CFX 96 Touch Real-Time PCR Thermocycler/ Detection System (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... eluted in 8 μl and in vitro transcribed (with the beads) at 37 °C overnight for linear amplification using the T7 High Yield RNA polymerase IVT kit (NEB). Following IVT ...
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bioRxiv - Cancer Biology 2023Quote: ... adapter ligation and PCR amplification (9 cycles) was performed using NEBNext Ultra II directional RNA library prep kit for Illumina protocol (NEB, E7760) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... and PCR amplification to prepare cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, USA). Library preparation and next-generation sequencing were outsourced to Rhelixa Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... several hundred cells exhibiting highest fluorescent intensity in the corresponding channel were collected and subjected to whole genome amplification using a commercially available whole genome amplification kit (WGA, New England BioLabs) followed by PCR amplification ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was treated with amplification-grade DNase I (NEB) and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Amplification and Library Generation kit from NEB (New England Biolabs Inc.) to isolate ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB), and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... and PCR library amplification (KAPA library amplification primers; NEB Ultra 2× master mix), followed by double-sided SPRI size selection and a final 0.9× SPRI purification ...
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bioRxiv - Microbiology 2020Quote: DNA amplification was performed using the Q5 DNA polymerase kit (New England Biolabs, USA) and the corresponding primers (Table S3 ...
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bioRxiv - Microbiology 2020Quote: A template-switching 5’ rapid amplification of cDNA ends (5’-RACE) kit (New England BioLabs) was used to map the transcription start site of rflP ...
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bioRxiv - Molecular Biology 2019Quote: ... adaptor ligation and PCR amplification with NebNext Ultra library preparation kit for Illumina (NEB #E7370S) following the manufacture protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs) was used to generate cDNA from the extraction ...
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bioRxiv - Microbiology 2023Quote: ... A RNA cleanup kit (Monarch RNA Cleanup Kit T2050, New England Biolabs, France) was used to further clean the RNA samples ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and subsequent PCR amplification was done with OneTaq RT-PCR Kit (New England Biolabs). Primers ...
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bioRxiv - Synthetic Biology 2019Quote: ... transcription templates were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of the plasmids to include a 5’ region upstream of the T7 promoter and a 3’ region ending with either 3WJdB or the T7 terminator with the primers listed in Supporting Data File 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... va mtSSB ΔMTS by PCR amplification and ligation (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554). The plasmid insert was sequence-verified and then transformed into E ...
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bioRxiv - Biochemistry 2019Quote: ... The PCR amplification (Q5 polymerase (NEB)) consisted of 30 cycles (95°C ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplification by Taq polymerase (NEB) using primers for internal regions of KL ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification using Phusion polymerase (NEB) with selective primes (fwd ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries underwent PCR amplification (NEB-Next High-Fidelity 2x PCR Master Mix ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified with Monarch RNA Cleanup kit (NEB). PNK-treated and non-treated RNAs were annealed to splints (1:1 ratio ...
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Genomics 2022Quote: Monarch RNA Cleanup kit (NEB)
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bioRxiv - Genomics 2023Quote: ... or RNA cleanup kit (NEB) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... following the manufacturer’s protocol A160141 v01 for library amplification using the NEBNext Ultra II kit (NEB E7645S).
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bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Genetics 2020Quote: ... DNA libraries were then prepared by PCR amplification using NEBNext High-Fidelity PCR kit (New England Biolabs) in the presence of barcoded PCR primers (sequences provided in Ref ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed using the Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, UK) and the final reaction contained 250 ng of template DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplification of the library was performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g. ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL from both ssDNA and reverse-transcribed RNA samples were used to template PCR amplification using Taq polymerase (NEB). The resulting amplicons were treated with either ClaI or DraI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... from RNA-seq samples was used as input for PCR amplification with Phusion High-Fidelity DNA polymerase (New England Biolabs) and UPF1 specific primers that flank the regulatory loop sequence (Forward ...
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bioRxiv - Biochemistry 2022Quote: The DNA templates for the different RNA designs were produced by PCR amplification using Phusion High-Fidelity DNA polymerase (NEB) of double stranded gene fragments (gBlocks ...
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bioRxiv - Genomics 2020Quote: ... RNA was purified using a Monarch RNA purification kit (NEB). RNA quality was evaluated with an Agilent Tape station ...
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bioRxiv - Bioengineering 2021Quote: ... RNA was purified using a Monarch RNA Cleanup Kit (NEB) and quantified on a Nanodrop spectrophotometer (Thermo).
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted using the Monarch RNA extraction kit (BioLabs) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated using Monarch RNA prep kit (NEB). Reverse transcription was performed on 0.5 µg DNase-treated total RNA using Lunascript RT (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was purified (Monarch® RNA Cleanup Kit, T2040L, NEB) from fractions and samples were stored as above ...
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bioRxiv - Microbiology 2023Quote: ... RNA was processed using Monarch RNA Cleanup Kit (NEB T2030L) and quantified on a Nanodrop Spectrophotometer 2000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was purified using the MonarchR RNA Cleanup Kit (NEB). The integrity of the purified RNA was determined by running RNA in a 1% agarose gel ...
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bioRxiv - Evolutionary Biology 2021Quote: Amplification used Phusion High Fidelity polymerase (NEB) and the following cycling protocol ...
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bioRxiv - Microbiology 2019Quote: ... Subsequent amplification was digested with DpnI (NEB) for 1 hr at 37°C followed by treatment with ClonExpress (Vazyme ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR amplification followed the recommended protocol (NEB) with 12 PCR cycles and a 50 sec ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification with Phusion Polymerase (New England Biolabs) included a 30 s denaturation at 98°C ...