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Citations for New England Biolabs :
1 - 50 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Protein digestion was achieved by sequential addition of endopeptidase Lys-C (BioLabs) and trypsin (Pierce™ ...
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bioRxiv - Biochemistry 2024Quote: ... Bound proteins were eluted by digestion with 500 units micrococcal nuclease (New England Biolabs) for 10 minutes at 37°C in 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Biochemistry 2023Quote: ... EcoRV digestion (NEB), and ion exchange of the excised fragment by HiTrap DEAE (147-bp ‘601’ ...
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bioRxiv - Molecular Biology 2021Quote: ... digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB). The RNA transcripts were extracted using Trizol (Thermo ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... After cleaning the plasmid digestion using the Monarch DNA Cleanup Kit (NEB, #T1030), the DNA concentration of the linearized plasmid was measured to determine the number of DNA molecules in the stock solution ...
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bioRxiv - Genetics 2022Quote: ... NruI enzymatic digestion (NEB) and DNA sequencing (Eurofins Genomics ...
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bioRxiv - Cell Biology 2022Quote: ... DpnI digestion (NEB #R0176S), PCR cleanup (NEB #T1030S) ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... DpnI digestion (NEB #R0176S), PCR cleanup (NEB #T1030S) ...
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bioRxiv - Cell Biology 2022Quote: ... 1X digestion buffer and 50 U digestion enzyme (all New England Biolabs®) were used to digest the plasmid DNA ...
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bioRxiv - Neuroscience 2021Quote: ... and the digestion product was purified using Monarch PCR & DNA Cleanup Kit (NEB, T1030S). Following the preparation of both the backbone vector and insert ...
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid was blunted by digestion with Quick Blunting™ Kit (NEB, USA) and ligated to re-circularize the plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... followed digestion with XmaI (NEB) at 37°C overnight leaving a 5’CCGGG methylation signature ...
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bioRxiv - Synthetic Biology 2021Quote: ... followed by DpnI digestion (NEB) to cut methylated template DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... digestion and Gibson assembly (NEB). To generate mRNA ...
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bioRxiv - Neuroscience 2021Quote: ... in the digestion buffer (NEB 3.1 or 2.1 ...
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bioRxiv - Developmental Biology 2021Quote: ... after digestion with BbsI (NEB) using Rapid Ligation Kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... endonuclease digestion (New England Biolabs), ligation (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... re-digestion with BamHI (NEB) and purification with a PCR clean-up kit (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified digestion products were ligated to Esp3I-treated LentiCRISPR-v2 using Gibson assembly kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified the rest of the digestion product with Monarch Nucleic Acid Purification Kit (NEB). We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB, M0649) and LC-MS analysis.
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bioRxiv - Synthetic Biology 2021Quote: ... Backbone plasmids were purified from gel after digestion using the Monarch DNA Gel Extraction Kit (NEB). Ligations were carried out in 96-well PCR plates by mixing 0.2 μL of digested plasmid (3-9 ng μL−1 ...
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bioRxiv - Biochemistry 2024Quote: ... a pET-derived vector that is normally used for T7 RNA polymerase-dependent protein expression (AVA421) was linearized by digestion with XbaI (NEB) in a reaction containing 1X CutSmart buffer and 0.5 U/μl XbaI to be used for run-off transcription using T7 RNA polymerase to transcribe the first 26 nucleotides following the T7 promoter sequence to generate the standard model RNA substrate ...
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bioRxiv - Microbiology 2021Quote: ... Double digestion with EcoRI (NEB, R0101S) and EcoRV (NEB ...
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bioRxiv - Genetics 2021Quote: ... Following DpnI digestion (New England Biolabs, the PCR product was purified (BioBasic Inc.) ...
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bioRxiv - Genetics 2020Quote: ... Following digestion with BanI (R0118S, NEB), the products were run on a 2% TBE gel (100V for 40 minutes) ...
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bioRxiv - Developmental Biology 2022Quote: ... linearized by EcoRI/BamHI digestion (NEB) to generate pTetOne-Fgfr2-IRES-sfGFP (Fig ...
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bioRxiv - Genomics 2021Quote: ... Secondary enzyme digestion with DpnII (NEB) was done on the 3C library at 37°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion using DpnII (New England Biolabs) was then performed for 1 h at 37°C ...
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bioRxiv - Genomics 2022Quote: ... After overnight digestion with HindIII (NEB), DNA ends were labeled with Biotin-14-DCTP (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... After USER® enzyme digestion (NEB), DNA fragments were amplified (oligonucleotides 10829 and 10830 in Supplementary Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... and digestion with BseYI enzyme (NEB), the cross-linked chromatin was ligated using T4 DNA ligase (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... digestion and T4 ligase (NEB #M0202S) reaction ...
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bioRxiv - Genomics 2023Quote: ... Addgene# 60517),56 using SfiI restriction digestion (NEB) to create pSBbi-GN::KCNQ1 ...
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bioRxiv - Genomics 2023Quote: ... After overnight-digestion with HindIII (NEB), DNA-ends were labeled with Biotin-14-DCTP (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The washed beads were resuspended in 100ul DNaseI digestion mix (1X DNase1 digestion buffer with 5ul DNase1 (NEB)) and incubated at 37°C for 20min ...
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bioRxiv - Molecular Biology 2021Quote: ... pARDRsP was digested with Xcm1 digestion (NEB) to release the RFPscePAC and ligated into the linearized pMA-RQ-70int ...
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bioRxiv - Cancer Biology 2019Quote: ... using the digestion sites of BbsI (NEB) to generate pTsgRNA-Cas9 ...
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bioRxiv - Molecular Biology 2019Quote: Restriction digestions — using EcoRI and XhoI (NEB) — and ligations — using T4 DNA ligase (ThermoFisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... After digestion with DpnI (Cat. R0176S, NEB), the PCR products were purified using Ampure beads.
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bioRxiv - Genomics 2021Quote: ... Primary enzyme digestion with HindIII-HF (NEB) was done at 37°C for 18h ...
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bioRxiv - Microbiology 2021Quote: ... After digestion by DpnI (New England Biolabs) and purification of PCR products (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Following digestion with BspHI and XhoI (NEB) and clean up ...
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bioRxiv - Cancer Biology 2021Quote: ... digestion and Gibson Assembly (New England BioLabs); control sgRNA ...
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bioRxiv - Microbiology 2020Quote: ... Following digestion with NdeI and BamHI (NEB) and clean up ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by a double digestion system (NEB) and ligation (Promega ...
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bioRxiv - Microbiology 2022Quote: Plasmids were linearized by AflII digestion (NEB) and then transformed into PichiaPink™ Strain one (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Plasmids were linearized by AflII digestion (NEB) and then transformed into PichiaPink™ Strain one (Invitrogen ...