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Citations for New England Biolabs :
1 - 50 of 9696 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... MnCl2 to a concentration of 1 mM and NEBuffer for Protein MetalloPhosphatases (NEB) diluted to a 1X concentration were added to both tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (NewEngland Biolabs) as a standard ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Input and pulldown libraries were sequenced with an Illumina NextSeq550 with 42bp per read using the NextSeq 500/550 High Output 75-cycle v2.5 kit.
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bioRxiv - Cancer Biology 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentrations determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced on an Illumina NextSeq 550 ...
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bioRxiv - Genetics 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs, #M0251S) by 10% SDS-PAGE gel electrophoresis stained with Coomassie blue (Bio-rad).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (New England Biolabs) as a standard ...
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bioRxiv - Synthetic Biology 2024Quote: ... The DNA concentration was determined using NEBnext library quant kit (NEB) and capillary electrophoresis (Bioanalyzer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and preloaded with guide DNA or guide RNA in a 1:1 ratio at 37°C for 15 min ...
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bioRxiv - Synthetic Biology 2023Quote: Protein expression was performed with the PURExpress In Vitro Protein Synthesis kit (E6800, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Lactis Protein Expression kit (New England Biolabs, UK) was used with the pKLAC2 vector ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a final concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and incubated with the respective guide at 37°C for 15 min in a 1:1 ratio ...
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bioRxiv - Genomics 2023Quote: ... Library concentration was quantified using NEBNext Library Quant Kit for Illumina (NEB E7630). Pooled libraries were sequenced using NextSeq500 in 75 bp paired-end mode ...
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bioRxiv - Genomics 2023Quote: ... Library concentration was quantified using NEBNext Library Quant Kit for Illumina (NEB E7630). Pooled libraries were sequenced using NextSeq500 in 75 bp paired-end mode ...
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bioRxiv - Genetics 2023Quote: ... and their concentration was determined using the NEBNext Lib Quant Kit (E7630, NEB). The libraries were sequenced on an Illumina NextSeq 550 ...
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bioRxiv - Cancer Biology 2020Quote: ... on ice for 30 min and protein concentration measured by BCA assay (7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple) ...
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bioRxiv - Molecular Biology 2021Quote: ... EcoRI sites underlined) and different concentration of recombinant His-SET8 protein (0 to 1.4 μM, New England Biolabs) were incubated for 10 min on ice in 1× GRB binding buffer [20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... Library concentration was determined by NEBNext Library Quant Kit for Illumina (New England Biolabs). Paired end sequencing (76 cycles ...
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bioRxiv - Cancer Biology 2022Quote: ... the library concentration was qPCR measured by NEBNext Library Quant Kit (New England BioLabs) using QuantStudio 5 Real-Time PCR Systems (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2019Quote: The PURExpress in vitro protein synthesis kit (New England BioLabs) was used to transcribe and translate Were-1-Fluc ...
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bioRxiv - Microbiology 2022Quote: Experiments with PURExpress in vitro protein synthesis kit (NEB, E6800) were performed as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: We used PURExpress In Vitro Protein Synthesis Kit (NEB, E6800L) and the reaction was set up with the following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used to translate the synthetic transcriptome described above ...
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bioRxiv - Microbiology 2023Quote: ... proteins were removed using the Monarch RNA Cleanup Kit (NEB) and 5’-mono-phosphorylated v51_mut_S templates were specifically digested with a terminator 5’-phosphate-dependent exonuclease (Biosearch technologies ...
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bioRxiv - Cell Biology 2023Quote: ... kit and co-injected with Cas9 protein (New England Biolabs) into one-cell stage zebrafish eggs ...
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bioRxiv - Cell Biology 2020Quote: ... DNA concentration were measured using the NEBNext® Library Quant Kit for Illumina (E7630L, NEB). Each 50 μl PCR reaction was composed of 0.5 μM primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Final concentration was measured by qPCR using the NEBNext Library Quant Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Library concentration and quality control were determined using NEBNext Library Quant Kit (New England Biolabs) and Agilent High Sensitivity D1000 ScreenTape System (Agilent ...
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bioRxiv - Developmental Biology 2022Quote: ... The molar library concentration was assessed using NEBNext Library Quant Kit for Illumina (NEB, E7630). Libraries were diluted to 4nM ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA concentrations were quantified by qPCR with the NEBNext Library Quant Kit for Illumina (NEB). Libraries were pooled and sequenced for 28-90 paired-end nucleotide reads to an average depth of ∼70,000 mapped fragments per cell.
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bioRxiv - Developmental Biology 2024Quote: ... Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs) or by Qubit (Thermo) ...
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bioRxiv - Biochemistry 2022Quote: 30 uL of tagged Fluc-WT proteins were incubated with Factor Xa (NEW ENGLAND BioLabs, final concentration 67 ug/mL) for 2 hours or overnight on ice.
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bioRxiv - Bioengineering 2020Quote: IVPS was performed using PURExpress In Vitro Protein Synthesis Kit (NEB) with supplements (16 U RNase inhibitor (NEB) ...
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bioRxiv - Microbiology 2023Quote: The PURExpress ΔRibosome In Vitro Protein Synthesis Kit (New England Biolabs) was used for in vitro translation assays ...
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bioRxiv - Molecular Biology 2024Quote: ... high concentration (NEB). The ligated cDNA was amplified with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Cancer Biology 2019Quote: ... After determining accurate concentrations by quantitative PCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 15-20% PhiX spike-in ...
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bioRxiv - Bioengineering 2022Quote: ... and library concentrations were measured with the NEBNext Library Quant Kit (New England Biolabs, Ipswich, MA). The libraries were pooled to a final concentration of 2 nM and run on a NextSeq sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of libraries was determined using NEBNext library Quant kit for Illumina (NEB, Cat# E7630). Libraries were then pooled and sequenced by Macrogen on Ilumina Hiseq X-ten platform ...
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bioRxiv - Synthetic Biology 2024Quote: ... library concentrations were quantified with qPCR using the NEBNext Library Quant Kit for Illumina (NEB, E7630S), and pre- and post-selection libraries were combined as two pools ...
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bioRxiv - Molecular Biology 2024Quote: ... while library concentration was determined using the NEBNext Library Quant Kit for Illumina (NEB, Cat# E7630). All input RNA had RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2019Quote: ... were performed with 5-10 μM final protein concentration and 10 μM dye (SNAP-Surface Alexa Fluor488 (New England Biolabs S9129S) or SNAP-Surface Alexa Fluor647 (New England Biolabs S9136S) ...
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described in the instructions with minor modifications ...
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bioRxiv - Microbiology 2023Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described previously (31) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μl of the gRNA mixture was mixed with Cas9-NLS protein (final concentration of 5 μM. New England Biolabs, Ipswich, USA), 2M KCl (final concentration of 300mM) ...
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bioRxiv - Genomics 2023Quote: ... we did not measure the DNA concentrations) with a NEBNext Ultra II DNA kit (New England Biolabs) according to the manufacturer’s protocol and sequenced as 100-bp single-end reads on an Illumina NovaSeq 6000 instrument.
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bioRxiv - Genetics 2023Quote: ... Molar concentration of libraries was determined using NEBNext® Library Quant Kit for Illumina® (NEB, #E7630) following manufacturer’s protocol for 6 Standards ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified amplicons was quantified by qPCR using the NEBNext Illumina Quant kit (NEB). The forward and reverse reads were demultiplexed and fused barcodes were quantified through alignment against custom barcode and primer sequences using Bowtie2 (V2.3.4.1 ...