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Citations for New England Biolabs :
1 - 50 of 802 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 1 x protease inhibitor cocktail (NEB), 2 mM N-ethylmaleimide (NEM) ...
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bioRxiv - Biochemistry 2019Quote: ... 1X protease inhibitor cocktail (New England Biolabs)] ...
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bioRxiv - Biochemistry 2019Quote: ... and 3 protease inhibitor cocktail tablets (New England Biolabs) were added to the cell suspension ...
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bioRxiv - Neuroscience 2022Quote: ... 1X protease inhibitor cocktail ± lambda protein phosphatase (New England Biolabs) as per the manufacturer’s instructions and incubated at 30°C for one hour.
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bioRxiv - Plant Biology 2022Quote: ... 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 x Protease Inhibitor Cocktail (NEB) as described before (Sun et al. ...
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bioRxiv - Plant Biology 2021Quote: ... 1 x Protease Inhibitor Cocktail) with appropriate amount of MNase (New England Biolabs) and incubated at 37 °C for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... 1× protease inhibitor cocktail) and incubated with 12,000 gel units/ml micrococcal nuclease (NEB, M0247S) at 37 °C with frequently mixing to digest chromatin to lengths of approximately 100-to-1,000 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% sodium deoxycholate) with 1X protease inhibitor cocktail and 920 U of murine RNase inhibitor (M0314L, New England Biolabs, UK). Resuspended cell pellets were further lysed on ice by passing through an 18G blunt needle 10 times ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µl 100× Protease inhibitor cocktail and 1 µl of CviKI-1 (5 U/100,000 nuclei, NEB R0710S) were added ...
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bioRxiv - Genomics 2020Quote: ... 0.5% NP40 and 1x protease inhibitors (P8107S, NEB) on ice for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM β-ME) supplied with protease inhibitors and an RNase inhibitor (NEB, M0314L) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM EDTA and 40 U/ml RNAse inhibitor (New England Biolabs) at 4 °C for 5 min to remove non-specifically associated proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 4% DMSO (Biolabs), 200 nM of each primer kdpAB(37) ...
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bioRxiv - Genomics 2022Quote: ... 1.5 µL 100x BSA (NEB, #B9000S), 5 µL 10x T4 DNA ligase reaction buffer (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.75 μL DMSO (NEB), 0.25 μL Pfx Polymerase (Thermo) ...
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bioRxiv - Cell Biology 2023Quote: ... or for 1 h in serum-free DMEM with a cocktail of heparinase I (NEB, P0735S), heparinase II (NEB ...
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bioRxiv - Genomics 2019Quote: ... 20 U RNasin Ribonuclease inhibitor and 1× protease inhibitor mix) and further treated with 100 U of DNase I (NEB) for 20 min on ice ...
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bioRxiv - Biochemistry 2022Quote: ... 1 µL 100x BSA (New England Biolabs), 1 µL of MNase (stock at 25 U/µL ...
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bioRxiv - Genetics 2021Quote: ... with or without DMSO (NEB), and the following protocol ...
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bioRxiv - Biophysics 2023Quote: ... 7.5% DMSO (New England Biolabs), 200 ng forward megaprimer ...
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bioRxiv - Molecular Biology 2023Quote: ... and S2) or EDTA (25 mM final concentration) plus 1.8-units Protease K (all other Figures, New England Biolabs), and incubating at 25°C for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM NaCl, 2 mM EDTA, 1% NP40, 0.1%SDS, 0.5 mM DTT, 40 U RNase inhibitor [New England Biolabs, cat#M0314L] ...
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bioRxiv - Genetics 2023Quote: ... 0.2μl 100X Bovine Serum Albumin (New England Biolabs, B9000S), 200μl 10X NEBuffer 2.1 (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer IIIb (3% Triton X-100, 1x T4 ligase buffer, 1x protease inhibitors; NEB, Sigma-Aldrich) was added to a final volume of 1 ml and sample was incubated for 15 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... pellets dried and resuspended in 100 µL RNase-free water containing 40 U murine RNase inhibitor (NEB). Following quantification by Nanodrop ...
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bioRxiv - Microbiology 2022Quote: ... DMSO (0.75 μl /sample) (New England Biolabs), molecular grade water (7.25 μl /sample) ...
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bioRxiv - Genetics 2020Quote: ... and 1.5 μl DMSO (New England Biolabs) per 50 μl reaction ...
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bioRxiv - Biochemistry 2022Quote: ... 5% dimethyl sulfoxide (DMSO, New England BioLabs), 0.12% triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... or 0.2% DMSO (New England BioLabs B0515A) as control ...
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bioRxiv - Neuroscience 2021Quote: ... Cell pellets from three 10 cm plates were pooled together and lysed in 1 mL of NP40 buffer (supplemented with cOmplete™ Mini protease inhibitor, 1mM PMSF, NEB Murine RNase inhibitor and RNaseIN) by incubating at 4 °C for 30 min with occasional pipetting to mix ...
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bioRxiv - Systems Biology 2020Quote: Single hematopoietic stem and progenitor cells were index-sorted into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Immunology 2021Quote: ... into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µM Verapamil in DMSO (Spirochrome) and of 0.6 µM SNAP-Cell TMR-Star in DMSO (New England Biolabs (S9105S) for 120 mins as per manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.6 µL of 100% DMSO (NEB #12611P), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.6 µL of 100% DMSO (NEB #12611P), with the following thermal cycle condition ...
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bioRxiv - Cell Biology 2019Quote: ... 50mM Tris (pH 8.5-8.8)) containing protease and phosphatase inhibitors (cOmplete Mini, PhosSTOP, Roche; 2mM Sodium Orthovanadate, NEB), sonicated to shear chromatin and centrifuged to remove cellular debris ...
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bioRxiv - Molecular Biology 2023Quote: ... The nuclei pellet was resuspended in buffer IV (160 µl annealed oligos, 1.2x T4 ligase buffer, 6,000 U T4 ligase, 1x protease inhibitors; NEB) to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB ...
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bioRxiv - Immunology 2019Quote: ... Single cells were flow-sorted into 96-well plates containing 2 µL of nuclease-free water with 0.2% Triton X-100 and 4 U murine RNase inhibitor (NEB), centrifuged ...
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bioRxiv - Genetics 2020Quote: 300 intestinal stem and Paneth cells were sorted into 2 μl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB). RNA was reverse transcribed (Invitrogen ...
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Enhancer heterogeneity of lung neuroendocrine tumors reveals sensitivity to FGF signaling inhibitionbioRxiv - Cancer Biology 2023Quote: ... After precipitation RNA was resuspended in appropriate volume of Rnase free water supplemented with RNA inhibitors (New England Biolabs). RNA samples were stored at -80°C until further use ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were then resuspended in Wash Buffer supplemented with TEV protease and Murine RNase Inhibitor (New England Biolabs), and the bait-associated complexes were eluted for 1-1.5 hours ...
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bioRxiv - Biochemistry 2023Quote: ... SNAP-Cell TMR Star (2 mM in DMSO, NEB), and SNAP-Cell Block (2 mM in DMSO ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP-Cell Block (2 mM in DMSO, NEB) were prepared and stored as aliquots in the dark at –20 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were resuspended in Elution Buffer (Wash Buffer supplemented with TEV protease and Murine RNase Inhibitor (New England Biolabs)) and the bound Enp1-TAP containing complexes were eluted for 1.5 – 2 hours ...
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bioRxiv - Microbiology 2019Quote: ... followed by size exclusion chromatography (HiLoad 16/60 Superdex 200) using the following buffers: lysis buffer (wash buffer supplemented with protease inhibitor, DNase I [NEB]), wash buffer (20mM HEPES-KOH pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... by removing media and washing cells with cold PBS and subsequently scraped into 50 – 200 μl (depending on dish size) lysis buffer containing protease inhibitor (#5872, NEB). The sample was then left on ice for at least 20 mins and subsequently spun down at 20,000 x g for 10 mins at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% DMSO and 1 U Phusion high fidelty polymerase (NEB) in 1x buffer provided by the manufacturer ...