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Citations for New England Biolabs :
1 - 50 of 2230 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Microbiology 2020Quote: ... anti-maltose binding protein (MPB) (New England BioLabs #E8032L) and anti-outer membrane protein (OmpA ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μL 10× Poly(A) Polymerase Reaction Buffer and 1 μL Poly(A) Polymerase (NEB) for poly(A ...
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bioRxiv - Biophysics 2021Quote: ... 100μg/ml single stranded DNA binding protein (gp32) from T4 (NEB), 277μg/ml T4 DNA clamp ...
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bioRxiv - Biochemistry 2019Quote: ... Immunoblotting was performed using HRP-conjugated anti-maltose binding protein (MBP) monoclonal antibody at 1:10000 dilution (New England Biolabs, #E8038) to verify equal expression levels of each construct.
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bioRxiv - Plant Biology 2020Quote: ... The NRPD1-Maltose Binding Protein (MBP) fusion protein was affinity purified using Amylose resin (New England Biolabs) and eluted with 20 mM maltose ...
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bioRxiv - Genomics 2021Quote: - Extreme Thermostable Single-Stranded DNA Binding Protein (ET SSB, 500 ng/μL, NEB) was tested in 4 different amounts ...
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bioRxiv - Molecular Biology 2022Quote: ... 4.2 ng/μl extreme thermostable single-stranded DNA binding protein (New England Biolabs) and 0.02 U/μl SuperFi DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... samples were enriched for mRNA using oligo-dT binding and magnetic separation using the NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs). Samples were reverse transcribed using the NEBNext RNA First and Second Strand Synthesis Modules (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated antibody binding was detected using TMB substrate (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... was expressed as maltose-binding protein-tagged fusion protein using the pMAL(tm)c5X-vector (New England Biolabs, Ipswich, USA). The coding DNA sequence was amplified by PCR using gene-specific primers (for primer sequences ...
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bioRxiv - Cell Biology 2019Quote: ... were expressed as fusion proteins with an N-terminal maltose binding protein (MBP)-tag using the pMALTMc5x-vector (New England Biolabs). Cloning was mediated by the addition of the restriction sites XmnI/PstI to the ends of gene fragments PCR-amplified from P ...
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bioRxiv - Biochemistry 2022Quote: ... CBP (Chitin Binding Protein, produced by N. Martín in Dr. Casanova’s lab, New England Biolabs Protocol) was used as a secondary antibody at 1:300 to detect chitin and visualize the tracheal branches ...
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bioRxiv - Cell Biology 2020Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75-thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Cancer Biology 2021Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75- thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Molecular Biology 2023Quote: Poly-A tailing was performed using poly-A polymerase (NEB) for 30 minutes at 37 degrees ...
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bioRxiv - Microbiology 2021Quote: ... 9 Neuraminidase A (P0722, NEB, 4 units/µg protein). 10 µg glycoprotein ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAs that were amplified without a poly-A tail were poly-adenylated using poly-A polymerase (NEB M0276) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2019Quote: ... 115 amino acids) fused to the C-terminus of maltose binding protein (MBP) in pMALX vector (New England Biolabs), MBP-5-HT3A-ICD-pMALX ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Poly-adenylation followed using Escherichia coli Poly(A) Polymerase (New England Biolabs) for 30 minutes at 37 °C and products were further cleaned with RNA clean & concentrator 5 (Zymo Research ...
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bioRxiv - Biochemistry 2021Quote: pMAL-c4E vectors carrying in-frame fusions of the EFR cytoplasmic domain with the N-terminal maltose-binding protein (MBP) tag were transformed into Rosetta 2 cells (NEB) for recombinant protein expression ...
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bioRxiv - Biophysics 2019Quote: ... was generated as a fusion construct with N-terminal maltose binding protein (MBP) as we described previously (45) using the pMAL-c2x (New England Biolabs) variant pMALX (46) ...
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bioRxiv - Microbiology 2021Quote: ... and cloned to the C terminus of a 11 His-tagged maltose-binding protein in a pMAL-C5X vector (New England Biolabs) separated by a cleavage site for tobacco etch virus protease ...
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bioRxiv - Immunology 2020Quote: ... The HDR templates were mixed at fourfold molar excess with a single-strand DNA binding protein (ET SSB, NEB, M2401S) and heated at 95°C for 10 minutes to achieve singlestranded donor oligonucleotides (ssODNs ...
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Biochemistry 2022Quote: ... and LaTR were fused to the C-terminus of the maltose binding protein (MBP) in the pMAL-c2x vector (New England Biolabs). Precisely ...
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bioRxiv - Genomics 2022Quote: ... for 2 hours and then crosslinked with p19 siRNA binding protein (1 μg/ml in depc-PBS; New England Biolabs) or anti-N6-methyladenosine (m6A ...
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bioRxiv - Biophysics 2021Quote: ... and poly(A)-tailed at their 3’ ends with poly(A) polymerase (NEB), following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... and a maltose binding protein (MBP) (65) and Factor Xa sequence derived from pMAL-c5X vector (New England Biolabs, Ipswich, MA) with a V313A mutation to be consistent with the native E ...
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bioRxiv - Molecular Biology 2022Quote: ... and α1-3,4 fucosidase (New England Biolabs, 4 U/μg protein). All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... 3-4-points were induced in the miR-29a seed binding site using the Q5 Site Directed Mutagenesis Kit (NEB, Cat#E0554S). 20 ng of recombinant dual-luciferase plasmid and 6 pmol of either miR-29a mimic or scrambled control were mixed with 100 μL Opti-MEM containing 1 μL lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Poly-A RNA enrichment was performed using NEBNext Poly(A) kit (New England BioLabs). Fragments were end-modified with adaptors using NEBNext Multiplex Oligos for Illumina Set 2 (New England BioLabs) ...
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bioRxiv - Microbiology 2024Quote: ... Poly(A) mRNA was isolated via NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) or the Ribo-Zero rRNA removal Kit (Illumina) ...
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bioRxiv - Biochemistry 2020Quote: 20 μg of protein from SK-RC-39 cells were treated with N-glycosidase F (PNGase F) and Endoglycosidase H (Endo H) (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... coli Poly(A) polymerase (NEB) and incubated for 10 minutes at 37°C with interval mixing (2000 RPM for 15 seconds every 3 minutes) ...
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bioRxiv - Neuroscience 2021Quote: ... coli Poly-A Polymerase (NEB) as previously described (23 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli Poly(A) polymerase (NEB), and incubating for 10 minutes at 37°C with interval mixing (2000 RPM for 15 seconds every 3 minutes) ...
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bioRxiv - Molecular Biology 2019Quote: ... coli Poly(A) Polymerase (NEB). The reaction was incubated for 5 min and stopped with 0.1 M EDTA ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... coli Poly(A) Polymerase (NEB) to improve translation efficiency ...
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bioRxiv - Microbiology 2020Quote: ... coli Poly(A) Polymerase (NEB), according to manufacturer instructions ...
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bioRxiv - Zoology 2022Quote: ... coli Poly(A) Polymerase (NEB). The fibroblasts were transfected with srRNA using Lipofectamine™ MessengerMax™ (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... coli poly(A) polymerase (NEB) and reverse transcribed with SuperScript III (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... coli Poly(A) Polymerase (NEB) before further clean-up (MEGAclear) ...
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bioRxiv - Biophysics 2023Quote: ... coli poly(A) polymerase (NEB), following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... coli Poly-A Polymerase (NEB) for 1 hour at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... coli Poly(A) polymerase (NEB), and reserve transcribed into DNA using Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... coli Poly(A) Polymerase (NEB) to make the spike-ins compatible with the NGS library preparation ...