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Citations for New England Biolabs :
1 - 50 of 1661 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... was isolated from cDNA of devil peripheral blood mononuclear cells (PBMCs) by PCR using Q5® Hotstart High-Fidelity 2X Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Immunology 2021Quote: ... was isolated from cDNA of devil peripheral blood mononuclear cells (PBMCs) by PCR using Q5® Hotstart High-Fidelity 2X Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genetics 2020Quote: ... 100 ng of DNA isolated from peripheral blood was digested with the methylation-sensitive HpaII enzyme (New England Biolabs, Ipswich, MA, USA), as originally described (39) ...
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bioRxiv - Genomics 2023Quote: ... DNA extraction from the blood samples was performed using the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB, T3050) following the manufacturer’s protocol (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted with Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB), digested with DpnI overnight at 37°C ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from blood (QiaAmp DNA Mini Blood Kit, Qiagen; or Monarch Genomic DNA purification kit, NEB) or from perfused organs homogenized (Fisher Bead Mill 4 ...
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bioRxiv - Genomics 2023Quote: ... cahirinus animal using a Monarch HMW DNA Extraction Kit for Cells & Blood (T3050, New England Biolabs, Ipswich MA) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2023Quote: ... and PacBio sequencing were prepared using the NEB Monarch HMW DNA Extraction Kit for Cells and Blood (NEB #T3050) using the manufacturer’s protocol for fresh blood with slight modifications as we have previously described (28) ...
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bioRxiv - Genetics 2024Quote: ... was extracted via the UHMW DNA extraction protocol of the Nanobind CBB Big DNA kit (Circulomics) or via the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs) and quantified using Qubit dsDNA Broad Range kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: DNA was isolated from 100 µL of whole blood using the blood protocol from the Monarch® Genomic DNA Purification Kit (New England Biolabs, Australia). In addition ...
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bioRxiv - Molecular Biology 2024Quote: ... an optional PBS wash followed by Red Blood Cell lysis step was included (10 mins at RT) prior to hypotonic lysis with Nuclei Prep Buffer (NEB, Cat.T3052) and Rnase digestion ...
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bioRxiv - Genomics 2024Quote: ... high-molecular-weight (HMW) DNA was extracted from cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps ...
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bioRxiv - Genomics 2023Quote: ... Samples were then processed following the NEB Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, Ipswich, MA, Cat#T3050L).
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bioRxiv - Molecular Biology 2020Quote: ... Total blood RNA from DBS was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, USA) with a modified protocol ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Genomics 2021Quote: ... High-molecular weight DNA was extracted from blood with a Bionano Plant Tissue DNA isolation kit (Bionano Genomics) and digested with Nt.BspQI nickase (New England Biolabs). After labeling and staining ...
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bioRxiv - Microbiology 2024Quote: ... Colony PCR was performed on Salmonella-positive colonies on blood agar using Taq DNA polymerase (New England Biolabs®) with an annealing temperature of 55°C and an extension time of 2.5 min ...
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bioRxiv - Microbiology 2021Quote: ... cholerae and phages was extracted using commercially available kits (Qiagen DNeasy Blood and Tissue or Monarch genomic DNA purification kit (New England BioLabs). For preparation of phage DNA ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA of 1×108 blood stage was extracted for conventional PCR amplification with a Q5® High-Fidelity DNA polymerase (New England Biolabs) from the interference inserts corresponding to the sense and anti-sense fragments of a portion of 429 bp AKT-like genomic sequence of T ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we isolated DNA from tissue using the Qiagen DNeasy Blood and Tissue kit (Valencia, CA, USA) and created genomic libraries using the NEBNext Ultra II kit (New England BioLabs; Ipswich, MA, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Platinum-GP cells (cell Biolabs, Inc) were transfected with a plasmid encoding the codon optimized SARS-COV-2 Spike gene from strain Wuhan-Hu-1 (GenBank ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent cell permeable Snap-Cell substrates (NEB) were used in the following concentrations ...
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bioRxiv - Molecular Biology 2020Quote: Transfected cells were stained with SNAP-Cell® TMR-Star or SNAP-Cell 647-SiR (NEB), both diluted 1:500 with complete DMEM ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were first labeled with cell-permeable substrate SNAP-Cell Fluorescein (New England Biolabs, Cat. # S9107S) overnight at 240 nM concentrations ...
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bioRxiv - Biophysics 2021Quote: ... coli cells (NEB) and was purified by maxiprep ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (NEB) and validated by sequencing using the universal primer U6-Forward.
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bioRxiv - Bioengineering 2019Quote: ... coli cells (NEB)
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bioRxiv - Immunology 2021Quote: ... coli cells (NEB), and the isolated plasmids were verified through sanger sequencing.
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bioRxiv - Microbiology 2021Quote: ... coli cells (NEB). All constructs were N-terminally truncated to remove the transmembrane helix (α1N ...
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bioRxiv - Bioengineering 2021Quote: ... coli cells (NEB). Constructs were validated by Sanger sequencing.
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bioRxiv - Bioengineering 2021Quote: ... coli cells (NEB).
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bioRxiv - Bioengineering 2021Quote: ... coli cell (NEB). Cells were grown at 37°C to log phase to achieve OD 0.8 ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell (NEB) for expression ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell (NEB) for expression ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell (NEB) as follows ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli cells (NEB) to generate the final barcoded piggyBac pool (final identifier P58) ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (NEB) and expressed as GST-fusion proteins ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (NEB). Recombinant clones were sequenced using the universal primers M13-Forward and M13-Reverse.
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bioRxiv - Biochemistry 2022Quote: ... coli cells (NEB) and expressed as a His-tagged protein in LB media supplemented with 60 µg/mL of kanamycin ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells (NEB), from which demethylated pSG01 was obtained for all downstream applications to increase the efficiency of B ...
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bioRxiv - Immunology 2020Quote: ... coli cells (NEB). Cells were grown in LB medium at 37°C to an optical density at 600 nm (OD600 ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (NEB) transformed with TmrAB was incubated for 16 hours overnight with shaking at 37°C in 10 mL of Luria Broth (LB ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (NEB) and underwent antibiotic selection on Kanamycin plates for 16 hours at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... coli cells (NEB) containing the recombinant protein expression plasmid were grown at 30°C and induced with 1 mM isopropyl-1-thio-β-d-galactopyranoside at 19°C for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... coli cells (NEB) using 0.25 mM IPTG for 16 hours at 18 °C ...
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bioRxiv - Biochemistry 2020Quote: ... DH10B cells (NEB) were transformed with the pColE1 reporter plasmid and plated on LB-kanamycin agar (50μg/mL) ...