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Citations for New England Biolabs :
1 - 50 of 112 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-PDGF-Ra (rabbit, New England Biolabs, 1:400 dilution), anti-APC (clone CC1 ...
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bioRxiv - Systems Biology 2019Quote: ... immunoprecipitated receptors were deglycosylated with PNGase F (New England Biolabs), before resuspending in LDS sample buffer and running on NuPAGE 4-12% Bis Tris precast gels ...
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bioRxiv - Cell Biology 2020Quote: ... CLIP-Cell TMR star substrate or CLIP-Surface 488 substrate (NEB S9219 and S9232) was reacted with dCas9-CLIP proteins at 37°C for 60min ...
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bioRxiv - Neuroscience 2022Quote: ... Receptor cDNAs were amplified by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs) from cDNA of mixed-stage populations of wild-type C ...
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bioRxiv - Neuroscience 2023Quote: ... 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs). All DNA was sequence verified using Sanger nucleotide sequencing (Retrogen ...
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bioRxiv - Microbiology 2020Quote: ... The TMR-Star substrate (New England Biolabs) was added at 250 nM ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent cell permeable Snap-Cell substrates (NEB) were used in the following concentrations ...
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bioRxiv - Cell Biology 2021Quote: ... Receptors were surface labelled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... Mutations were generated in the rat P2X2 receptor using the Q5 Site-Directed Mutagenesis Kit (NEB). Each mutation was verified by DNA sequencing and subcloned in pWPT-EF1α-P2X2-GCaMP6s-IRES-DsRed2 or pWPT-EF1α-P2X2-GCaMP6s-P2A-mScarlet lentiviral vectors.
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Biophysics 2020Quote: Gaussia and Cypridina substrates were obtained from NEB or its original supplier ...
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (NEB) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Biochemistry 2023Quote: ... in vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer with 5 μg RNA substrate (1.8 knt. Same substrate of MTase-Glo experiment) and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 1 μM methyltransferase or no enzyme as a negative control ...
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bioRxiv - Molecular Biology 2019Quote: ... The substrates were amplified using Phusion Polymerase PCR (NEB). The linear double-stranded molecules were denatured and renatured to produce both the parental linear molecules and a circle with nicks on each strand nearly half-way around the circle which were then ligated by Taq Ligase (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.3 µM fluorescent SNAP substrate (TMR Star, NEB, S9105) was added along with the primary antibodies
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (S9105S, NEB), for 30 min anaerobically ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-Cell 647-SiR substrate (S9102S New England Biolabs) was used to reveal SNAP-tag following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB) based on the domain boundaries reported in (28) ...
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bioRxiv - Microbiology 2019Quote: ... Signal was detected with Clarity™ ECL Blotting Substrate (NEB) and on a GE Imagequant LAS 4000 mini ...
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bioRxiv - Biophysics 2020Quote: ... for the substrate-binding domain mutants and βamHI-AlwnI (NEB) for the transmembrane domain mutants (Appendix) ...
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bioRxiv - Molecular Biology 2023Quote: ... or ΦX174 RF I DNA (dsDNA substrate, NEB, Ipswich MA) into buffer containing 50 mM Bis-Tris pH 6.0 ...
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bioRxiv - Microbiology 2023Quote: ... MARCH2 chimeras containing the TM domains from either human MARCH4 or human transferrin receptor (TR) were generated using the NEBuilder HiFi DNA assembly kit (New England Biolabs) and the primers listed in Supplemental Table S2 ...
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bioRxiv - Biophysics 2021Quote: ... After 24 hours SNAP-Cell 647-SiR substrate (New England Biolabs) was added to the medium at a concentration of 3 μM ...
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bioRxiv - Biochemistry 2020Quote: Translocation substrates were synthesized in vitro using the PURExpress system (NEB) in the presence of [35S]-methionine for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... the substrates used are 5’-end-labeled with T4 PNK (NEB) in the presence of gamma 32P-ATP ...
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bioRxiv - Microbiology 2019Quote: ... The double-stranded DNA substrate pUC19 (New England Biolabs, MA, USA) was linearized by digesting with PstI (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Circular ssDNA (M13MP18) substrate (0.5 µg, New England Biolabs, MA, USA) was combined with 4 pmol of each cellular nuclease alone and in combination ...
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bioRxiv - Cell Biology 2019Quote: ... cells were labeled with Oregon-Green SNAP substrate (New England Biolabs) at 4 µM final concentration for labeling of the newly synthesized CENP-A molecules ...
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bioRxiv - Biochemistry 2021Quote: ... the pre-crRNA substrates were 5′-radiolabeled with T4 PNK (NEB) in the presence of gamma 32P-ATP ...
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bioRxiv - Biochemistry 2023Quote: ... The dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 2.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 5 ng/µL (~0.83 nM) ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated antibody binding was detected using TMB substrate (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: 5’-end 32P-labelled DNA substrates were generated using T4 PNK (NEB) and [ɣ-32P]ATP (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... DNA substrates were 5’ terminally labelled with T4 Polynucleotide Kinase (NEB, M0201S) and ATP-γ-32P (PerkinElmer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... The substrate was then treated with 50 U of uracil DNA glycosylase (NEB) for 2 h at 37°C to form the abasic site ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 3 times with PBS-T and TMB substrate (Denovo Biolabs) was added for development of color ...
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bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
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bioRxiv - Molecular Biology 2022Quote: DNA substrates for gel shift assays were amplified by PCR using Q5 Polymerase (NEB) and purified using a DNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of the DNA substrate were digested with 30U of Nb.BbvCI (from NEB) for 2 h at 37 °C ...
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bioRxiv - Genetics 2020Quote: DNA substrates were generated using Q5 High-Fidelity DNA Polymerase (M0491S; New England BioLabs) to PCR-amplify pDEST-BLRR with TIDE-1-fwd and TIDE-1-rev (Supplementary Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... followed by detection of ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs), anti-GST and anti-ubiquitin (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... The chemical substrates were SNAP- tag ligands (SNAP surface 549 - BG 549 [NEB, S9112S]) and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB ...
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bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
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bioRxiv - Biochemistry 2021Quote: ... or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates; New England Biolabs, P0702S). All samples were solubilised for 12 h at 37°C and then sonicated prior to resolution by SDS-PAGE (10% or 16% PAGE ...
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bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
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bioRxiv - Neuroscience 2020Quote: ... PBT was removed and SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) added ...
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bioRxiv - Biochemistry 2021Quote: ... The deaminations were detected by treating the substrates with Uracil DNA Glycosylase (New England Biolabs) and heating under alkaline conditions ...
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bioRxiv - Microbiology 2021Quote: ... 5μM diphosphorylated 20mer RNA substrate was pre-treated with 0.5U/μl RNA 5’ Pyrophosphohydrolase (NEB) in 1× NEBuffer 2 (NEB ...