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Citations for New England Biolabs :
1 - 50 of 5204 citations for PCR Strips since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... nuclei were sorted into a tube of a PCR tube strip containing 5 μL 1X CutSmart buffer (NEB, B7204S) per well ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.1% Tween) with 3% BSA and then incubating the strips in 1:2000 dilution of monoclonal anti-MPB-HRP antibody (NEB, Inc, USA) in a blocking buffer for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were PCR purified using Monarch® PCR DNA Cleanup Kit (New England BioLabs) and eluted with water ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by genotyping PCR using Phusion PCR protocol (NEB) with primer pair of forward ...
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bioRxiv - Genomics 2021Quote: ... Post-PCR each PCR aliquot was exonuclease I (NEB) treated ...
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bioRxiv - Biochemistry 2023Quote: ... and amplified by PCR using Pfusion PCR polymerase (NEB) with specific primers or random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: PCR with the teltail and P1 PCR primers was performed using Q5 PCR enzyme mix (NEB, #M0494) and the following conditions using 0.1 μl DNA-bound beads ...
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bioRxiv - Molecular Biology 2021Quote: PCR was performed with Taq polymerase PCR (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... PCR clean-up (Monarch PCR and DNA Cleanup Kit, NEB) and gel extraction (Monarch Gel Extraction Kit ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were purified (Monarch PCR Cleanup Kit (NEB, #T1030S)) and restriction digested with unique 5’ SpeI and 3’ PspXI sites ...
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bioRxiv - Microbiology 2020Quote: ... PCR was done using LongAmp® Taq PCR Kit (NEB) from 40 ng of QMA0248 S ...
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bioRxiv - Genetics 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... before PCR using NEB 2xphusion PCR mastermix (NEB, cat #M0531S).
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bioRxiv - Molecular Biology 2019Quote: PCR products were amplified by PCR using the Q5 (NEB®) high-fidelity polymerase following standard conditions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (NEB) for all fragments ...
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bioRxiv - Molecular Biology 2023Quote: All restriction enzymes were purchased from NEB and PCR amplification was performed with Q5 High-Fidelity PCR (NEB) following the manufacturer’s recommendations ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... were PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB) and cloned into pCFD4 (Addgene 49411 ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Immunology 2023Quote: PCR was performed in a 40 μl PCR reaction containing 1x Q5 HotStart Hifi PCR master mix (New England Biolabs), 0.4 μM of Illumina adapter PCR primers (ILM_p5_PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... twelve-cycle PCR reactions (Phusion High Fidelity PCR kit, New England Biolabs), using primers that added Flow Cell binding sequences ...
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bioRxiv - Biochemistry 2022Quote: ... Following which the PCR products were purified by PCR cleanup kit (NEB) and a second PCR was done with primers containing the READ1 ...
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bioRxiv - Immunology 2022Quote: ... a quantitative PCR reaction (10 µl 2 x PCR master mix (NEB), 1 x SYBR green (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR reactions were purified with Monarch PCR & DNA Cleanup Kit (NEB, T1030L). For the hHBB-Fluc control mRNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were generated using the 2x Q5 PCR Master Mix (NEB) in accordance with manufacturer instructions ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Resulting PCR product was purified using Monarch PCR purification kit (NEB, USA) and were cloned into pCS2+ vector using restriction enzymes BamH1 and SnaB1 based cloning to generate pCS2-Satb2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR product was purified using Monarch PCR & Cleanup Kit (NEB T1030S) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were cleaned (Monarch PCR & DNA Cleanup Kit, NEB cat. T1030S) and Sanger-sequenced ...
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bioRxiv - Microbiology 2022Quote: ... Products were PCR cleaned up with Monarch PCR & DNA cleanup kit (NEB), ligated into pCR2.1-TOPO vector (Thermo fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were subsequently PCR amplified using Phusion HF PCR Master Mix (NEB) with the following PCR program ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were then purified by PCR & DNA Cleanup Kit (NEB). STR PCR products were ligated with the pClone007 simple vector (Tsingke) ...
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bioRxiv - Biochemistry 2023Quote: RT-PCR was completed using Taq DNA polymerase (Taq PCR kit, NEB), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were purified using the Monarch PCR Purification Kit (NEB # T1030L) and eluted in 10µl elution buffer.
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Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix (NEB, M0531) and the enhancer deletion validation primer set (Table S5) ...
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bioRxiv - Cell Biology 2019Quote: ... and PCR (NEB #M0491S) at the region of indel (forward primer ...
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bioRxiv - Immunology 2019Quote: ... PCR nucleotides (NEB, MA) and Gag-specific reverse primer (Table S3) ...
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bioRxiv - Microbiology 2021Quote: ... using Phusion PCR (NEB) and primers BAC16_downstream_of_GFP_STOP_overhang_plus_Zeo_3ʹ and BAC16_upstream_of_GFP_ATG_antisense_strand_overhang_plus_EM7_P_start and inserting it into BAC16 via recombination ...
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bioRxiv - Cell Biology 2022Quote: ... PCR cleanup (NEB #T1030S), NEBuilder HiFi assembly (NEB #E2621S) ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... PCR cleanup (NEB #T1030S), assembly with the NEBuilder HiFi kit (NEB #E2621S) ...
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bioRxiv - Microbiology 2024Quote: ... colony PCRs (GOTAQ, NEB) were performed on positive E ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmented DNA was PCR amplified using custom i5 and i7 PCR primers and Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB). PCR conditions were ...
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bioRxiv - Genetics 2022Quote: ... Two independent lines were successfully established and PCR validated through single fly PCR (Gloor et al., 1993) using OneTaq PCR master mix (NEB #M0271L). Primers for PCR were designed to flank the integration site (primer sequences ...
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bioRxiv - Microbiology 2019Quote: ... PCR was done with the Phusion High-Fidelity PCR kit (New England BioLabs) with 40ng of gDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cleaned up using the Monarch PCR Cleanup kit (T1030L, NEB). A double digest using PacI and AscI was done on the PCR products and the pcs2+MCS-P2A-sfGFP plasmid ...