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Citations for New England Biolabs :
1 - 50 of 7713 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Genetics 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 25 µL PCR were set up with 1X PCR Buffer (NEB, Ipswich, MA, USA) containing 1.5 mM MgCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... For PCRs we used Phusion High-Fiedlity PCR Master Mix with HF Buffer (NEB) and reactions were run as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and contained 5 μL of Q5 PCR buffer (NEB), 0.0625 μL of 100 μM universal forward primer ...
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bioRxiv - Genomics 2019Quote: ... and contained 5 μL of Q5 PCR buffer (NEB), 0.0625 μL of 100 μM universal forward primer ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR reaction contained 1x Q5 Reaction Buffer (NEB), a detergent-free buffer containing 2.0 mM Mg++ at final 1x concentration ...
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bioRxiv - Genetics 2020Quote: PCR mixture per reaction: 10 µl HF Buffer (NEB), 1.25 µl 10-µM forward primer ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR reaction consisted of 1x ThermoPol Buffer (NEB), 200 µM dNTPs ...
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bioRxiv - Microbiology 2023Quote: ... The PCR was completed using 1x reaction buffer (NEB), 200 µM dNTPs ...
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bioRxiv - Cancer Biology 2019Quote: ... All PCRs were performed with Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB).
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bioRxiv - Genetics 2019Quote: ... and amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) to produce sub-pools for Gibson assembly (NEB) ...
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were performed using Phusion high HF buffer (NEB) and standard PCR conditions (98°C for 30s ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were performed using Phusion high HF buffer (NEB) and standard PCR conditions (98°C for 30s ...
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bioRxiv - Molecular Biology 2020Quote: Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) was used to amplify the novel and annotated transcript using custom primers (IDT ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions (50 µl) containing 1 × Q5 reaction buffer (NEB), 200 µM dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μl PCRs contained 1X ThermoPol Buffer (New England Biolabs), 250 nM PRA1_NoMod.F or PRA1_shrt.F ...
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bioRxiv - Genetics 2022Quote: ... Each PCR consisted of: 20 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 2 µL 10 µM i5 indexing adapter ...
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bioRxiv - Biochemistry 2023Quote: ... Each PCR consisted of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB) in a total volume of 20ul ...
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bioRxiv - Synthetic Biology 2024Quote: All PCRs were carried out using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), gel extractions using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmented DNA was PCR amplified using custom i5 and i7 PCR primers and Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB). PCR conditions were ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was done with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Preamp PCR: Beads were resuspended in 200 µl PCR mix (20 µl 5x phusion HF buffer (NEB); 1 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... and PCRs were performed via Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB #M0531S). After PCR clean-up (Promega #A9281 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were generated using the OneTaq Quick-Load 2x PCR Master Mix with Standard Buffer (NEB) in accordance with manufacturer instructions with C ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) or RedTaq DNA Polymerase 2X Master Mix (VWR Life Science ...
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bioRxiv - Immunology 2023Quote: ... 17 PCR cycles were performed using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532) with primers and template oligo each at a concentration of 10uM ...
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bioRxiv - Developmental Biology 2019Quote: ... E7.5) into 96 well PCR plates containing 2.5μl of methylase reaction buffer (1× M.CviPI Reaction buffer (NEB), 2U M.CviPI (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... PCR amplification was performed in “GC” optimized Pfusion buffer (NEB # M0532S) using a gradient of annealing temperatures and oligonucleotides that anneal only within the unique open reading frame regions of each Lsm locus ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), and 400 nM forward and reverse Fluidigm PCR primers in a 20 μL reaction volume ...
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bioRxiv - Biophysics 2019Quote: ... 96 × 50 ul PCR reactions in 1 × ThermoPol reaction buffer (NEB) were prepared using template (0.01 ng µL-1) ...
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bioRxiv - Microbiology 2021Quote: ... The 25□µL final reaction volume contained 1X PCR Buffer (NEB), 3□mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions contained 5X Q5 reaction buffer (New England Biolabs, UK), Q5® High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions contained 5X Q5 reaction buffer (New England Biolabs, UK), Q5® High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were composed of 10 µL Q5 reaction buffer (NEB), 1 µL 10 mM dNTPs (NEB) ...
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bioRxiv - Systems Biology 2024Quote: ... We setup 50uL PCR reactions with 1x OneTaq GC Buffer (NEB), 400uM of additional MgSO_4 ...
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bioRxiv - Cancer Biology 2021Quote: ... The regular PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs). The PCR products that targeted exon18-10 and exon9-10 were then combined for each sample and run on 2% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB M0531) and PCR cycling at 98°C for 30s ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs). All inserts were verified by sequencing (GENEWIZ) ...
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bioRxiv - Genomics 2020Quote: ... The final PCR amplification was performed using 2X Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) and final 0.1 μM of PE1.0 and corresponding multiplex PE2.0_MTX (Table S3) ...
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bioRxiv - Molecular Biology 2022Quote: ... tagmented DNA was PCR amplified using 1x Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB) and 1.25 μM i5 and i7 PCR primers (Nextera® Index Kit (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... treating each well with 100 μl of lysis buffer (0.45% NP40, 0.45% Tween20, 0.5x NEB Q5 PCR buffer, 0.1 mg/mL NEB Proteinase K) were incubated for 10 min at 55°C in and subsequently heated to 95°C for 10 min to inactivate the proteinase K ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and/or Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) (Table S2) ...
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bioRxiv - Neuroscience 2021Quote: ... PCRs were performed using OneTaq 2X Master Mix with Standard Buffer (NEB) using the following primers ...
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bioRxiv - Genetics 2022Quote: ... 25 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 20 µL nuclease-free water ...