Labshake search
Citations for New England Biolabs :
1 - 50 of 3636 citations for Oligodendrocyte transcription factor 1 OLIG1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis of the binding sites for the predicted Caudal and DEAF-1 transcription factors was performed sequentially with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) taking as a template the pGreenRabbit vector containing the FBti0019386 sequence following manufacturer’s instructions (25) ...
-
bioRxiv - Biophysics 2022Quote: ... 1 µL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
-
bioRxiv - Biophysics 2023Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
-
bioRxiv - Bioengineering 2024Quote: ... This fragment was cloned into a lentiviral expression plasmid we have previously described (31) encoding the Notch1 core linked to the tTA transcription factor using NEBuilder HiFi DNA Assembly (New England Biolabs). The resultant plasmid encoded constitutive expression of CII-synNotch receptor from an EF1α promoter and production of the puromycin N-acetyl-transferase transgene via the constitutive PGK promoter for antibiotic selection ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
-
bioRxiv - Bioengineering 2022Quote: ... Then CaCl2 was added to 2mM and 1 µL (about 1 µg) of Factor Xa (NEB) was added per 50 µg of protein and the samples were left at RT for 20-24h ...
-
bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
-
bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
-
bioRxiv - Plant Biology 2021Quote: ... Factor Xa (P8010S, New England BioLabs) or TEV protease (P8112S ...
-
bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
-
bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
-
bioRxiv - Cell Biology 2021Quote: All dox-inducible factors were generated by cloning the open reading frame of each factor into the pMINI vector (NEB) and then restricted with EcoRI or MfeI and inserted into the FUW-TetO expression vector ...
-
bioRxiv - Biophysics 2021Quote: ... In vitro transcription (NEB E2040S) was carried out according to the manufacturer’s instructions using 100 ng of DNA template ...
-
bioRxiv - Genomics 2023Quote: ... in vitro transcription (NEB E2040S) at 42°C for 8 hours in the presence of RNasin Ribonuclease Inhibitor (Promega N2111 ...
-
bioRxiv - Genomics 2023Quote: ... performed reverse transcription (NEB, E3010L) and ran qPCR (NEB ...
-
bioRxiv - Immunology 2021Quote: ... In-vitro-transcription was carried out using the Hi-Scribe RNA transcription kit (New England BioLabs). Briefly ...
-
bioRxiv - Systems Biology 2020Quote: ... Then in vitro transcription (NEB E2040S) followed by reverse transcription (Thermo Fisher EP7051 ...
-
bioRxiv - Microbiology 2021Quote: ... For reverse transcription (RT) 1 µg RNA was digested with 2 U of DNase I (NEB). After heat inactivation of the DNase at 70 °C for 5 min ...
-
bioRxiv - Microbiology 2020Quote: ... the resin was treated with factor Xa protease (P8010L, NEB) overnight at 4°C ...
-
Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...
-
bioRxiv - Developmental Biology 2020Quote: ... the concentration of eluted MBP-mintbody was adjusted to 1 mg/ml and digested with 30 μg/ml Factor Xa (New England Biolabs) for 24 h ...
-
bioRxiv - Molecular Biology 2020Quote: ... Ribosome concentrations were calculated with a NanoDrop spectrophotometer assuming 1A260 = 60 μg ribosome mL−1 (conversion factor provided by New England Biolabs). This conversion factor was used to estimate the molecular mass of bacterial ribosomes ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of in vitro transcribed 300 nM sgRNA or trL (produced as described in in vitro transcription protocol above) and 1 μL of 1 μM Cas9 nuclease (NEB, M0386S) were mixed and incubated for 10 minutes at 25°C to allow for RNP formation ...
-
bioRxiv - Microbiology 2022Quote: ... cDNA were synthesised from 1 µg RNA by reverse transcription using LunaScript RT SuperMix Kit (NEB; E3010). qRT-PCR was performed using Luna Universal qPCR Master Mix (NEB ...
-
bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was with SuperScript III (NEB), and subsequent PCR amplification of the cDNA was with Phusion (NEB) ...
-
bioRxiv - Physiology 2020Quote: ... 5x transcription buffer (NEB, Hitchin, Hertfordshire, UK), 0.2 M dithiothreitol (DTT ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The transcriptions (1X template, 1X Homemade NEB Buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... MBP was cleaved from LH using Factor Xa (New England Biolabs) at a w/w ratio of 1% Factor Xa:LH ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by T7 in vitro transcription using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs, Ipswich, MA) and purification with Trizol (ThermoFisher Scientific ...
-
bioRxiv - Evolutionary Biology 2021Quote: Hybridization with the reverse transcription primers was performed by adding 1 uL of SR RT Primer (NEB E7333A) to the above reaction and incubating at 75 C for 5 minutes ...
-
bioRxiv - Biophysics 2020Quote: Each reverse transcription (RT) reaction was performed using 50 ng total RNA in 1 mM dNTPs (NEB N0447S), 10% DMSO (Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... In vitro transcription was performed with either HiScribe T7 ARCA mRNA kit or the HiScribe In Vitro Transcription Kit (NEB) according to the manufacturer’s recommendation ...
-
bioRxiv - Microbiology 2023Quote: ... Transcription and fluorescent labelling of RNA in vitro transcription reactions were carried out using a T7 RNA transcription kit (HiScribe T7 High Yield; New England Biolabs) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: In vitro transcription reactions were carried out using a T7 RNA transcription kit (HiScribe T7 High Yield; New England Biolabs) with the following modifications ...
-
bioRxiv - Genomics 2023Quote: ... A transcription reaction was then carried out on the purified and quantified dsDNA using the T7 HiScribe transcription kit (NEB). The T7 RNA Polymerase recognizes the T7 promoter region that seeds transcription of the adjacent 20-mer target sequence thus generating the targeting sgRNA for Cas9-mediated nicking of genomic DNA templates ...
-
bioRxiv - Genomics 2020Quote: ... Reverse transcription was performed using Protoscript II (NEB) [4 µl 5x NEB FirstStrand buffer (NEB ...
-
bioRxiv - Neuroscience 2020Quote: ... and a reverse transcription kit (NEB, Ipswich, MA) according to the manufacturer protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... Reverse transcription was performed using ProtoScript II (NEB). qPCR reactions were assembled with Luna Master Mix (NEB ...
-
bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... UK) were then synthesized through in vitro transcription of 1 mg template with T7 polymerases (M0251, New England Biolabs). The sections were fixated in 4% PFA ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 μg of total RNA was used in cDNA synthesis using ProtoScript II Reverse Transcription kit (NEB, Cat # e6560) as per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: The purified PCR products were used as templates for in vitro transcription of antisense and sense ssRNA by using the HiScribe™ T7 In Vitro Transcription Kit (NEB #E2030S ...
-
bioRxiv - Microbiology 2020Quote: ... Appropriate fractions were pooled and digested with factor Xa protease (New England Biolabs) in the presence of 2 mM CaCl2 at 4 °C overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... Transcription reactions were treated with RNase-free DNaseI (NEB) to digest the DNA template at 37°C for 1 hour ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transcription reactions were carried out with SP6 polymerase (NEB) for 1 hour at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... In vitro transcription reactions were treated with DNAse (NEB) for 1 hour at 37°C ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and +1 sites of the promoters controlling transcription of both RNAs were used to amplify the standard pUC19 plasmid (NEB). After PCR amplification samples were digested with DpnI (NEB ...
-
bioRxiv - Microbiology 2022Quote: ... Strand-specific CHIKV RNA primers were chosen from previous work [54] to amplify nucleotides 1-1560 of the CHIKV genome and used in reverse transcription reactions with the LunaScript8482 RT SuperMix Kit (NEB), followed by qPCR using the 2x qPCRBIO SyGreen Blue Mix (PCRBIOSYSTEMS) ...
-
bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...