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Citations for New England Biolabs :
1 - 50 of 1072 citations for Native Human CST3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... in its native reaction buffer (NEB) by incubating at 72°C for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the native barcodes were ligated with the Native Barcoding Expansion 96 (EXP-NBD196) and NEB Blunt/TA Ligase Master Mix (NEB, M0367) selecting a unique barcode per sample ...
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bioRxiv - Plant Biology 2023Quote: ... with the native ACO2 promoter using Gibson Assembly® (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 0.75 µl End-prepped DNA, 1 µl Native Barcode (Native Barcoding Expansion 96, EXP-NBD196) and 5 µl Blunt/TA Ligase Master Mix (NEB Blunt/TA Ligase Master Mix, M0367) were combined in a new reaction vessel and incubated for 20 minutes at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... The powder was dissolved in a cold native extraction buffer (NEB: Tris HCl 100 mM pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... repaired and end-prepped DNA was barcoded with Native Barcode by NEB Blunt/TA Ligase Master Mix (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... Chimeric NICDs were created from native NICDs using HiFi DNA Assembly (New England Biolabs). Boundaries for chimeric constructs are given in Table S3.
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bioRxiv - Genomics 2019Quote: ... Native barcode ligation was performed with NEB blunt/ TA ligase (New England Biolabs, MA, USA) and cleaned with 0.5x AMPure XP beads ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the native barcodes were attached using a NEBNext® Ultra™ II Ligation Module (NEB). Up to 23 samples were pooled for sequencing in the same flow cell (FLOMIN106 flow cell R9.4.1) ...
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bioRxiv - Microbiology 2023Quote: ... Native barcode adapters NBD04 were ligated in Blunt/TA Ligase Master Mix (M0367S, NEB, USA), and resulting product was AMPure XP beads purified before pooling to produce a 54 μl equimass pool ...
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bioRxiv - Microbiology 2023Quote: ... 2.5uL from a single native barcode and 25 µL Blunt/TA Ligase Mix (NEB, M0367S) were added ...
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bioRxiv - Genomics 2019Quote: ... Native barcodes were added by ligation using the Blunt/TA ligase master mix (New England Biolabs) and the ligation reaction was purified using 1 volume ratio of AMPure beads ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were quenched with native loading dye containing EDTA (New England Biolabs, Cat. no. #B7025S). Samples were loaded on a 0.7% agarose gel and run at 120 V in 45□mM (0.5× ...
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bioRxiv - Biochemistry 2021Quote: Native glycoproteins (10 μg) were digested with 1 U of IMPa (O-Glycoprotease, New England Biolabs) in 50 μL 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... and the fleQ gene and its native promoter were amplified using Phusion polymerase (New England BioLabs) with primers containing 20bp of homology to the mTn7/pMQ56 SmaI cut-site at their 5’ end ...
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bioRxiv - Microbiology 2024Quote: ... Barcodes (Native Barcoding Kit 96 (SQK-NBD109.96)) were ligated using Blunt/TA Ligase Master Mil (NEB). Barcoded libraries were purified using KAPA Pure Beads (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat ...
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bioRxiv - Biophysics 2024Quote: ... 10 µl of each sample was mixed with 4 µL native loading dye (1% Orange G (NEB) dissolved in 50% glycerol and 50% EMSA buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... native adapter ligation and cleaning steps were performed using NEBNext Quick Ligation Module (New England Biolabs, USA) kit and Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligation of the Native barcode was then performed by adding Blunt/TA Ligase Master Mix (NEB M0367) and a unique native barcode (Oxford Nanopore Technologies EXP-NBD196 ...
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bioRxiv - Genomics 2020Quote: ... instead of SuperScript IV (Thermo) and half volume native barcoding reactions using Blunt/TA Ligase Master Mix (NEB) instead of NEBNext Ultra II Ligation Module (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 3-5μg of native genomic DNA were dephosphorylated with 0.05U/μl of shrimp alkaline phosphatase (rSAP) (New England BioLabs) to reduce the amount of off-target molecules in the final SMRTbell® library ...
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bioRxiv - Plant Biology 2023Quote: ... The genomic DNA was barcoded, adding Native Barcoding Expansion (ONT, Oxford, UK) and Blunt/TA ligase mix (NEB) was added for 10 minutes at room temperature and purified with 1X AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2024Quote: ... 1.25 µL native ligation barcode (ONT SQK-NBD114-96) and 3 µL Blunt/TA Ligase Master Mix (NEB). The reaction was mixed by pipetting ...
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bioRxiv - Molecular Biology 2021Quote: ... 22.5 µl of cDNA was combined with 2.5 µl native barcode and 25 µl Blunt/TA Ligase Master Mix (New England Biolabs). Samples were incubated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... 22.5 µL of this was taken forward for native barcoding with the addition of 2.5 µL barcode and 25 µL Blunt/TA Ligase Master Mix (NEB) (final volume 50 µL) ...
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bioRxiv - Microbiology 2021Quote: ... End-prepared DNA was ligated with native barcode adapters NBD04 using Blunt/TA Ligase Master Mix (M0367S, NEB, USA). Following the barcode ligation reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... Native PCC 11901 genetic parts were amplified from genomic DNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Where necessary ...
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bioRxiv - Microbiology 2024Quote: ... 30 µL of the barcoded DNA was ligated onto 2.5 µL Native Ligation Adapter (NA, ONT SQK-NBD114-96) by addition of 12.5 µL NEBNext Quick Ligation Reaction Buffer (NEB) and 5 µL high conc ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... For ssg complementation we PCR amplified the ssg coding sequence and 100 bp upstream sequence (including the ssg native promoter) using Q5 DNA polymerase (New England Biolabs). We cloned this 1057 bp product into KpnI-BamHI digested pUC18T-miniTn7-Gent by Gibson assembly using NEBuilder HiFi Assembly master mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... 22.5 µl first strand cDNA was mixed with 2.5 µl Native Barcode (EXP-NBD104) and 25 µl Blunt/TA Ligase Mix (NEB, M0367S) and incubated in room temperature for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and barcoded using an Native Barcoding Expansion 96 kit (Oxford Nanopore Technologies) and a Blunt/TA Ligation Master Mix (New England Biolabs). Barcoded samples (96 ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 fmol of input cDNA were incubated for 20 minutes at room temperature (15–25°C) with the native barcodes and Blunt/TA Ligase Master Mix (New England Biolabs), followed by 10 minutes at 65°C.
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bioRxiv - Microbiology 2020Quote: ... the full length of MoMca1 and MoMca2 genes including their native promoter were amplified and inserted into pBGt (40) fungal expression vector by using the Gibson Assembly approach (NEB BioLabs). The pBGt-MoMca1Mca2 expression vector was transformed into ΔMomca1mca2 protoplasts and selected on gentamicin-containing plates ...
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bioRxiv - Microbiology 2020Quote: ... A 1.5kb product for STM2441 including the native promoter was generated from template genomic DNA using Q5 DNA polymerase (New England Biolabs) with an annealing temperature of 72°C and an extension time of 40s for 35 cycles ...
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bioRxiv - Genetics 2020Quote: ... plasmid containing native 3’-UTRs were generated by excision of the 2xMyc-ADH1 3’-UTR from each RRP4/40-Myc LEU2 CEN6 plasmid by restriction digestion and cloning of the native RRP4 or RRP40 3’-UTR into each plasmid using NEBuilder HiFi Assembly (New England BioLabs).
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bioRxiv - Microbiology 2023Quote: ... Barcodes from the Native Barcoding Expansion 1-12 & 13-24 from ONT were ligated using the NEBNext Ultra II Ligation Module (NEB). DNA was purified using Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2022Quote: ... the resulting products were firstly ligated with barcodes from the Native Barcoding Expansion kits by using NEB Blunt/TA Ligase Master Mix (New England Biolabs) and then with adapter from the Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Biochemistry 2023Quote: Both native and modified oligonucleotides were 5’-32P labeled by [γ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs) following manufactures recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... under its native promoter was cloned into the plasmid pACYC184 (p15A origin of replication) using Gibson assembly (New England Biolabs) generating plasmid JW371 ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat. R3575, EcoRI-HF NEB cat ...
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bioRxiv - Microbiology 2023Quote: ... individual barcodes were incorporated into the dA-tailed DNA using the EXP-NBD104 and EXP-NBD114 native barcoding expansion kit following the ONT protocol with NEB Blunt//TA Ligase Master Mix (New Englands Biolabs). Barcoded DNA samples were then equimolarly pooled ...
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bioRxiv - Plant Biology 2024Quote: ... One µL of the end- prepped DNA was amplicons were barcoded with 1 µL of Nanopore Native Barcode using 5 µL Blunt/TA ligase master mix (NEB) in total reaction volume of 10 µL for 20 min at 20 °C ...
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bioRxiv - Microbiology 2024Quote: ... Genes of interest were PCR amplified to include either their native or heterologous promoter and cloned using Gibson Assembly kit (NEB) into integration vectors pDG1662 (for insertion into the amyE locus) ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Molecular Biology 2021Quote: ... TIF3 alleles with the native promoter and terminator encoding C-terminal hexahistidine tagged-eIF4B or variants were Gibson-cloned (NEB, USA) into the BamHI site of single-copy vector pHLUM (21) ...