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Citations for New England Biolabs :
1 - 16 of 16 citations for Mycoplasma Pneumoniae Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... pneumoniae D39 chromosomal DNA by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs). The kanamycin cassette was amplified from pSt-K and the streptomycin cassette from pGMC5-SM-RFP-PFurA-GFP-streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae SGH10 genomic DNAas template and the Q5 high-fidelity DNA polymerase (New England Biolabs). Then ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Enzymatic antigen retrieval with proteinase K (Biolabs, 2μg/mL) was used prior to incubation with antibodies ...
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bioRxiv - Pathology 2020Quote: ... Heat mediated antigen retrieval pretreatment using 10mM Sodium Citrate buffer (Vectors Biolabs) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... and regularly tested for mycoplasma contamination (PCR-based assay from Minerva-Biolabs, Berlin, Germany).
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bioRxiv - Molecular Biology 2019Quote: ... Heat mediated antigen retrieval pretreatment using 10 mM Sodium Citrate buffer (Vectors Biolabs) were used ...
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bioRxiv - Genetics 2021Quote: MBP-Stwl antigen was purified from induced bacterial culture using Amylose Resin (NEB: E8021L). Briefly ...
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bioRxiv - Immunology 2021Quote: Antigen binding fragment (Fab) was generated by incubating IgG with LysC (New England Biolabs, Cat# P8109S) at a ratio of 1 μg LysC per 10mg IgG at 37°C for 18hrs ...
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bioRxiv - Immunology 2020Quote: ... Antigen binding fragment (Fab) was generated by incubating IgG with LysC (New England Biolabs, Cat# P8109S) at a ratio of 1μg LysC per 10mg IgG at 37°C for 18hrs ...
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bioRxiv - Bioengineering 2019Quote: ... mycoplasma genomes are released by digestion of the agarose matrix with three units per plug of β-Agarase I (NEB), and the DNA concentration is measured using an Epoch™ Microplate Spectrophotometer (BioTek™).
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bioRxiv - Immunology 2020Quote: ... Linker insertion and modifications in the antigen-bearing components were achieved using Q5 Site Directed Mutagenesis Kit (NEB). Custom DNA primers produced by Integrated DNA technologies (IDT) ...
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bioRxiv - Genomics 2020Quote: ... Cell lines were regularly tested for mycoplasma contamination and confirmed negative with PCR (NEB NebNext Q5 High Fidelity 2X Master Mix).
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bioRxiv - Biochemistry 2020Quote: ... pCSE2.6-hFc-XP or pCSE2.6-mFc-XP 68 where the respective single chain variable fragment of the antibodies or antigens were inserted by NcoI/NotI (NEB Biolabs) digestion ...
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bioRxiv - Biochemistry 2020Quote: ... pCSE2.6-hFc-XP or pCSE2.6-mFc-XP 68 where the respective single chain variable fragment of the antibodies or antigens were inserted by NcoI/NotI (NEB Biolabs) digestion ...
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bioRxiv - Immunology 2022Quote: ... the MPXVac-097 vaccine candidate construct encoding five monkeypox antigens in tandem was in vitro transcribed from linear DNA template using HiScribe T7 ARCA mRNA kit (NEB, Cat. No. E2060S) with 50% replacement of uridine by N1-methyl-pseudouridine (Tirlink ...