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Citations for New England Biolabs :
1 - 50 of 355 citations for Mouse IgG1 Isotype Control Antibody 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... or an appropriate isotype-matched control (polyclonal goat isotype IgG, NEB) was added at a final concentration of 10 μg/ml and incubated overnight ...
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bioRxiv - Immunology 2022Quote: ... As an isotype control an unspecific polyclonal rabbit antibody was used (NEB, Frankfurt, Germany). To block ORF8 during differentiation it was preincubated with the polyclonal rabbit anti-ORF8 antibody or isotype over night and added to the monocytes (t=0 ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse antibodies against MBP (NEB E8032L, 1:5000) and RAD51 (Novus Biologicals 14b4 ...
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bioRxiv - Biochemistry 2020Quote: ... with anti-Knbu/Kibu as the positive control using anti-butyryllysine antibody (PTM Biolabs, Cat# PTM-301) and histone H3 as the loading control using histone H3 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal antibodies against PAR (clone 10H, Tulip BioLabs, USA), anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Molecular Biology 2020Quote: ... antibody were incubated with 25 µL of magnetic anti-mouse beads (NEB) overnight ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with anti-mouse HRP antibody (1:5000, NEB 7076S) for 1h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Around 100ng of the RNA was kept as input control and stored at -80°C whereas the remaining amount was subjected to immunoprecipitation using m6A specific antibody (NEB #E1610S). Initially ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound antibodies were detected by incubation with anti-rabbit or anti-mouse DyLight 800-conjugated secondary antibodies (New England BioLabs). Slide images were acquired using an InnoScan 710-IR scanner (Innopsys ...
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bioRxiv - Molecular Biology 2023Quote: The linear ssDNA scaffold for our designs as the positive control and negative control was obtained by cutting circular M13mp18 DNA (New England Biolabs) at BamHI-HF and EcoRI-HF (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: mHSP70 or fetuin (New England Biolabs, positive control) were subjected to cleavage by PNGase F (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... or amylose resin (negative control; New England Biolabs) at 4°C for 3 h ...
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bioRxiv - Immunology 2020Quote: ... A positive control on the other hand was established by transforming DH5α bacteria with NEBuilder® Positive Control (New England Biolabs, USA). After transformation ...
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bioRxiv - Cell Biology 2021Quote: ... m6A-modified RNA spike-in controls (New England Biolabs) and a nontargeted region on the crRNA-targeted transcript ...
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bioRxiv - Cell Biology 2019Quote: ... Untreated controls were supplemented with GlycoBuffer3 (New England Biolabs). Reactions were stopped by treatment with SDS-PAGE sample buffer containing benzonase nuclease (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... As a control Escherichia coli ribosomes (P0763S, NEB, Australia) were used in 4 μL aliquots (approx ...
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bioRxiv - Molecular Biology 2023Quote: ... BamHI (Site 2) or HindIII (Control; New England Biolabs) overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: VH and VL sequences of candidate sequences were cloned into a pcDNA.3 based vector with dual CMV promotor harboring IgG1 heavy chain and light chain backbone using NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Bioengineering 2021Quote: ... were synthesized (Genewiz) based on the GenBank sequences with regions of overlap to restriction digested human IgG1 vectors for assembly cloning (NEB) to produce plasmids ...
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bioRxiv - Biochemistry 2021Quote: ... were synthesized (Genewiz) based on the GenBank sequences with regions of overlap to restriction digested human IgG1 vectors for assembly cloning (NEB) to produce plasmids ...
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bioRxiv - Immunology 2023Quote: ... including overhangs designed for subsequent cloning into expression vectors (IgG1, Igλ, Igκ).87 Sequence- and Ligation-Independent Cloning (SLIC) was performed using T4 DNA polymerase (New England Biolabs) and chemical competent Escherichia coli DH5α as previously described with a minor modification.36,88–90 In contrast to the published protocol a murine leader sequence encoded in the vector was used for secretion ...
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bioRxiv - Cell Biology 2020Quote: ... or positive controls doxorubicin (50 μM; New England Biolabs 5927) and antimycin A (10 μM ...
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bioRxiv - Immunology 2019Quote: ... pSV40-CLuc control plasmid was obtained from NEB (Ipswich, MA). These constructs were co-transfected to 293 cells by FugeneHD (Promega) ...
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bioRxiv - Developmental Biology 2020Quote: ... Controls were handled with same protocol without λ-phosphatase (NEB). Phospho-mass spectrometry was performed as previously described (Nagai et al. ...
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bioRxiv - Microbiology 2020Quote: ... Endo H controls: Cells were treated with Endo H (NEB, 1:10 in glycobuffer 3 ...
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bioRxiv - Cell Biology 2021Quote: ... The m6A-modified and unmodified control RNAs (New England Biolabs) were divided into fragmented RNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
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bioRxiv - Cancer Biology 2023Quote: ... A negative control treated overnight with RNaseH1 (New England Biolabs) was included ...
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bioRxiv - Immunology 2022Quote: ... with short homologies for Gibson assembly and cloned into human IgG1 or human IgL2 expression vectors using the NEB Hifi DNA Assembly mix (NEB, Cat#E2621L). Plasmid sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Cell Biology 2019Quote: ... then incubated with either mouse anti-MBP antibodies at a dilution of 1:5000 (New England Biolabs) or 1:2500 mouse anti-GFP antibodies (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Renilla luciferase was then ligated into pGL3-Control digested HindIII (NEB) to make pGL3-Renilla-FF ...
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bioRxiv - Developmental Biology 2021Quote: 4mC and 5mC spike-in controls were generated from pUC19 (NEB) PCR product synthesized with N4-methyl-dCTP (4mdCTP ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µg BSA dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 µl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: ... Unmethylated control plasmid was produced in dam− bacteria (New England BioLabs #C2925H) and methylation status was confirmed by DpnI and DpnII digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into the control plasmid using restriction enzyme XhoI (NEB, #R146L). pCAGGS-Cre plasmid was generated as described (Kranz et al. ...
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bioRxiv - Cell Biology 2021Quote: ... A positive control was prepared by substituting casein kinase II(NEB, P6010S) for galectin 3 in the reaction mixture.
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bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μg BSA per well dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 μl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Cell Biology 2023Quote: ... and pSNAPf-H2B control plasmid (Addgene #101124, New England Biolabs and Ana Egana) using KpnI and NotI ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used were: Alexa-680 goat anti-mouse IgG and Alexa-800 goat anti-rabbit IgG (New England Biolabs). For DRGN cultures ...
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bioRxiv - Systems Biology 2023Quote: ... Signals following a 1h incubation at room temperature with the appropriate HRP-conjugated secondary antibodies (anti-mouse NEB 7076S or anti-rabbit NEB 7074S) were acquired using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... the FLuc Control Template from the HiScribe T7 high yield RNA synthesis kit (NEB) was in vitro transcribed and purified as described the linearized pTXB1 vector.
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bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
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bioRxiv - Genomics 2020Quote: ... As a control plasmid pCMV6 plasmid (4.6kb) was linearized by digestion with NdeI (NEB, RO111). 180 ng control linearized plasmid DNA and 180 ng of 3C library was treated with either 0.5 Unit exonuclease V (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... Library concentration and quality control were determined using NEBNext Library Quant Kit (New England Biolabs) and Agilent High Sensitivity D1000 ScreenTape System (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... together with controls were subject to enzymatic digestion by PNGase F (P0708L, New England Biolabs), Endo H (P0702L ...