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Citations for New England Biolabs :
1 - 50 of 10000+ citations for Mouse Direct PCR Kit For Genotyping since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... followed by genotyping PCR using Phusion PCR protocol (NEB) with primer pair of forward ...
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bioRxiv - Bioengineering 2024Quote: ... Genotyping PCR was then conducted using Q5 polymerase (NEB) for genomic DNA and cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... Knock-in lines were confirmed by PCR genotyping (Phusion, NEB) and Sanger sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2022Quote: ... and further used as templates in genotyping PCR reactions (NEB OneTaq® Quick-Load® 2xMasterMix ...
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bioRxiv - Neuroscience 2024Quote: Genotyping protocols used either 2x Hotstart PCR Master mix (NEB) or 2x DreamTaq Green Master mix (Thermo Scientific K1081 ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR genotyping was performed using Q5 High-Fidelity 2X Master Mix (NEB) with the primer pairs listed in Table 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Genotyping was performed by PCR looking for acquired resistance to NcoI (NEB) cutting and selected clones were confirmed by immunoblotting for STING ...
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bioRxiv - Developmental Biology 2019Quote: ... The supernatant was used directly in genotyping PCR performed with LongAmp (NEB) or PrimeStar GXL (Clontech ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotyping-PCR was performed with Q5® High-Fidelity DNA Polymerase (NEB) with 98°C initial denaturation for 2 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Genotyping PCRs were performed with Phusion High-Fidelity DNA Polymerase (NEB; Cat#: M0530S), using primers surrounding the genomic target site.
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bioRxiv - Biochemistry 2022Quote: Genotyping was done by DNA PCR using Q5 (New England Biolabs cat # M0492L) and confirmed the presence of the expression cassettes.
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bioRxiv - Developmental Biology 2020Quote: ... Genotyping-PCR was performed with Q5® High-Fidelity DNA Polymerase (New England Biolabs) with 98 °C initial denaturation for 2 min ...
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bioRxiv - Genetics 2021Quote: ... PCR genotyping was carried out using either Gotaq or Q5 High Fidelity Polymerase (NEB). Annealing temperature and extension time were adjusted based on the melting temperature of the primers and size of the amplicon.
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bioRxiv - Cancer Biology 2021Quote: ... Genotyping PCRs were carried out using Q5 High-Fidelity DNA polymerase (New England Biolabs, #M0491) with an annealing temperature of 65°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping PCR on purified genomic DNA was performed with Phusion High-Fidelity DNA Polymerase (NEB). PCR products were checked by agarose gel for single band amplification of the appropriate size and then subjected to Sanger sequencing for definitive identification of the 8 bp ch200 DNA deletion in ythdf2 exon 4 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Endpoint PCR for genotyping or single guide RNA template preparation was carried out using Q5 (M0491 NEB) or Taq (M0267 NEB ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Endpoint PCR for genotyping or single guide RNA template preparation was carried out using Q5 (M0491 NEB) or Taq (M0267 NEB ...
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bioRxiv - Molecular Biology 2021Quote: Genotyping was performed by Q5 PCR (0.5 μl Q5® HiFi DNA Polymerase (New England Biolabs, M0491), 1xQ5 reaction buffer ...
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bioRxiv - Genetics 2021Quote: ... Genotyping was carried out by conventional PCR using crude DNA lysates and Taq polymerase (New England Biolabs). PCR products destined for cloning were made with Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Genetics 2020Quote: ... PCR genotyping was performed on extracted DNA in a total volume of 25 μl (12.5 μl NEB Q5 High-Fidelity Master Mix ...
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bioRxiv - Systems Biology 2021Quote: ... Positive clones were confirmed by genotyping the respective locus by colony PCR using Q5 Polymerase (NEB, Ipswitch, MA) as described in the next section.
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bioRxiv - Neuroscience 2024Quote: ... DNA lysate was used as genotyping PCR template with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and enhancer specific genotyping primer pair spanning the upstream and downstream Cas9-guide RNA cleavage sites (Table S19) ...
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bioRxiv - Genetics 2021Quote: Genotyping was performed with Q5 polymerase (NEB), primers fwd 5’-GTTAAAACAGTTTCTTAAAAAGAACAGGA-3’ and rev 5’-AGCAGAAGAAATGACTCAACATTTTG-3’ (annealing at 62°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was performed by agarose gel electrophoresis-mediated detection following PCR reaction by Taq polymerase (M0273, New England Biolabs). qPCR was performed using GoTaq® qPCR Master Mix (A6001 ...
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping PCR reactions (36 amplification cycles) were performed using Taq DNA Polymerase with ThermoPol Buffer (New England Biolabs, M0267L), dNTPs from Promega (U1511 ...
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bioRxiv - Cancer Biology 2024Quote: ... One genotyping PCR sample contained 10 µL OneTaq® Quick-Load® 2X Master Mix (M0486L, New England Biolabs), 1 µL 10 µM forward primer ...
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bioRxiv - Genetics 2022Quote: ... the F1 non-Unc or its non-Unc F2 offspring were lysed and subsequently subjected to PCR genotyping analysis using OneTaq 2x Master Mix (New England Biolabs) to determine the genotype of unc-5 and dpy-13 ...
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bioRxiv - Molecular Biology 2020Quote: ... Genotyping was performed with PCR amplifying 253bp across the cutting sites and looking for acquired resistance to MspA1l digestion (NEB). Positive clones were further confirmed by immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... and/or direct mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB) and cloned into the retroviral expression vector pQCXIZ by either restriction digestion and ligation ...
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bioRxiv - Immunology 2020Quote: ... We ran the genotyping PCR for the Rosa26 and the collagen status with the Phusion® High Fidelity DNA Polymerase (New England Biolabs) and the same primers as described previously and indicated in the Supplementary Table 2 [6].
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping dmrt2bumb4required treatment with the enzyme BaeGI (NEB R0708S), which digests only the wild-type amplicon.
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bioRxiv - Genomics 2021Quote: Individual clones were assessed for PDCL3P4 knockout by performing a genotyping PCR with Q5® High-Fidelity DNA Polymerase (New England BioLabs cat # M0492S) and primers placed outside of the gRNA cut sites ...
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bioRxiv - Genomics 2020Quote: ... Genotyping reactions were performed with OneTaq Hotstart Polymerase (NEB #M0481S).
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bioRxiv - Biochemistry 2023Quote: ... GST-tag HP1⍺ΔC construct was generated by site-direct mutagenesis kit (NEB) by deleting the amino acids (number 169 –181 ...
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bioRxiv - Developmental Biology 2022Quote: ... The Taq 2X Master Mix was used for genotyping (NEB, # M0270L).
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bioRxiv - Developmental Biology 2022Quote: ... The Taq 2X Master Mix was used for genotyping (NEB, # M0270L).
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bioRxiv - Biochemistry 2020Quote: ... Routine genotyping of mice was done by digestion with Hpy188III (NEB R0622) and analysis on a 2% agarose gel.
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bioRxiv - Developmental Biology 2021Quote: ... Sanger sequencing was performed on samples after genotyping with Phusion Polymerase (NEB) and PCR Purification Kit (QIAGEN) ...
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bioRxiv - Developmental Biology 2024Quote: ... Genotyping was performed with Quick-Load Taq 2X Master Mix (M0271L, BioLabs), on 2% agarose gel for the PCR bands ...
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bioRxiv - Microbiology 2022Quote: ... PCR clean-up (Monarch PCR and DNA Cleanup Kit, NEB) and gel extraction (Monarch Gel Extraction Kit ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were purified (Monarch PCR Cleanup Kit (NEB, #T1030S)) and restriction digested with unique 5’ SpeI and 3’ PspXI sites ...
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bioRxiv - Microbiology 2020Quote: ... PCR was done using LongAmp® Taq PCR Kit (NEB) from 40 ng of QMA0248 S ...
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bioRxiv - Genomics 2019Quote: ... Genotyping was performed using Phusion DNA polymerase (New England Biolabs Inc, Ipswich MA) with the primers listed in Supplemental Table 1 ...
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bioRxiv - Genomics 2021Quote: RNA-seq libraries were constructed at the ICBR Gene Expression & Genotyping Core Lab using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (NEB) for all fragments ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were PCR purified using Monarch® PCR DNA Cleanup Kit (New England BioLabs) and eluted with water ...
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bioRxiv - Molecular Biology 2021Quote: ... USA (NEB PCR Cloning Kit E1203S) were used for the sub-cloning of DNA fragments and to create plasmid templates for run-off in vitro transcription ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted by NEB PCR Cleanup Kit (NEB Cat. # T1030), followed by quality control steps using Qubit fluorometer and bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... AnTat1.1 sequence was obtained from AnTat1.1 specific cDNA from mouse infection D6 cloned into a pMiniT vector with the PCR Cloning Kit (NEB, E1202S). VSG-228 was partially amplified from VSG PCR (see below ...
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bioRxiv - Immunology 2020Quote: ... For genotyping hair samples were incubated in 250 μL of Direct PCR reagent and 20 mg/mL Proteinase K (New England Biolabs, P8102) for 1 h at 55°C ...