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Citations for New England Biolabs :
1 - 50 of 380 citations for Mouse Anti Mycoplasma pneumoniae P1 6532 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Nuclease P1 (NEB) were performed as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Nuclease P1 (NEB) treatment was performed as recommended by the supplier in a reaction volume of 10 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclease P1 (NEB) and Antarctic Phosphatase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclease P1 (NEB #M0660S); Alkaline phosphatase (NEB #M0290) ...
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bioRxiv - Microbiology 2023Quote: ... Nuclease P1 (New England Biolabs), RNAse T1 (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Two units Nuclease P1 (NEB) were added with 1/10 volume 0.1M ammonium acetate pH 5.3 for two hours at 45°C ...
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bioRxiv - Biochemistry 2023Quote: ... Five units of P1 nuclease (NEB) were added ...
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bioRxiv - Molecular Biology 2023Quote: ... each sample was treated with nuclease P1 (NEB) and Antarctic Phosphatase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 µL 10x Nuclease P1 buffer (NEB) for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The P1 expression plasmids were linearised by SacII (NEB), followed by the insertion of the 3CD PCR product into SacII-linearized P1 plasmid ...
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bioRxiv - Microbiology 2022Quote: ... The P1 expression plasmids were linearised by SacII (NEB), followed by the insertion of the 3CD PCR product into SacII-linearized P1 plasmid ...
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bioRxiv - Biochemistry 2022Quote: ... The P1 expression plasmid was linearised by SacII (NEB), and then the 3CD PCR product inserted ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae D39 chromosomal DNA by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs). The kanamycin cassette was amplified from pSt-K and the streptomycin cassette from pGMC5-SM-RFP-PFurA-GFP-streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae SGH10 genomic DNAas template and the Q5 high-fidelity DNA polymerase (New England Biolabs). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... After addition of 200 µl nuclease elution mix (NEB nuclease P1 buffer 1 x, MgCl2 5 mM, 0.5 µl NEB nuclease P1, 0.5 µg benzonase) samples were incubated over night at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... which were digested with 0.1 U Nuclease P1 (NEB, M0660) and 1.0 U calf intestinal alkaline phosphatase (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... digested to single nucleotides by 20U of Nuclease P1 (NEB) at 37°C for 30 min and dephosphorylated by 1U of Shrimp Alkaline Phosphatase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... and an aliquot was digested with P1 nuclease (NEB #M0660S) and analysed by LC-MS to identify the phosphorylation state of the nucleotide at the 5’ end.
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bioRxiv - Molecular Biology 2022Quote: ... and digested with either 10 U of nuclease P1 (NEB) or 10 U of heat-inactivated nuclease P1 for 1 hour at 37°C in a reaction volume of 100 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant RNA was then digested with nuclease P1 (NEB) and antarctic phosphatase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA samples were digested by 50 units nuclease P1 (NEB) in a 25-µL reaction mixture containing nuclease P1 buffer at 37°C for 2 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then treated with P1 nuclease (New England Biolabs) then rSAP (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: Optimal digestion by P1 nuclease (100 U/µL, New England Biolabs) required lysate to be adjusted to pH 6.5 before digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... 450 Units of P1 Nuclease (100 U/μL, New England Biolabs) were added per 30 μg of RNA and lysates were incubated at 37 °C for 1 hr with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Then gDNA was digested into nucleosides using the nuclease P1 (NEB) and Antarctic Phosphatase (NEB ...
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bioRxiv - Immunology 2022Quote: ... Eluted RNA was incubated with 180 units of nuclease P1 (NEB, M0660) in nuclease P1 buffer at 37 °C for 90 min ...
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bioRxiv - Microbiology 2024Quote: ... samples were split and treated with either 100 U Nuclease P1 (NEB) and 10 U Antarctic phosphatase ...
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bioRxiv - Genomics 2021Quote: ssDNA-specific Digestion: 20µL cfDNA was combined with 1µL 0.1x P1 (NEB, M0660S), 3µL NEBuffer r1.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were further processed and digested with P1 Nuclease (Cat#: M0660S, NEB). In vitro-transcribed 4B mRNA (firefly luciferase reporter (Bukhari et al. ...
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bioRxiv - Microbiology 2023Quote: 100 μM of compound was incubated with 0.02 units P1 nuclease (New England Biolabs) in P1 reaction buffer (50 mM sodium acetate pH 5.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Then the tRNA were digested with 10 U Nuclease P1 (New England Biolabs, MA, USA) at 37°C overnight followed by dephosphorylation using 10 U bacteria alkaline phosphatase (Thermofisher Scientific ...
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bioRxiv - Immunology 2023Quote: 200 ng extracted mRNA was digested into nucleosides by Nuclease P1 (1 U, NEB, M0660S) and shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Genomics 2019Quote: ... and ligated to indexed P1 adapters (one index per sample) using concentrated T4 DNA ligase (NEB). Ligated DNA was purified using AMPure XP magnetic beads ...
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bioRxiv - Molecular Biology 2022Quote: ... 5ul of each reaction was digested with P1 nuclease and treated with proteinase K (NEB #P8107S), prior to application to a TLC membrane (Machery-Nagel ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-MBP monoclonal (NEB), anti-mouse-HRP (Dianova) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting P1/P2 and P3/P4 amplicons were digested with AscI and FseI (New England Biolabs) and ligated in separate bimolecular reactions to an ermAM erythromycin resistance cassette with compatible 5’ AscI and 3’ FseI overhangs ...
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bioRxiv - Plant Biology 2023Quote: Blunt-ended DNA substrates were prepared by digesting the pGEX6-p1 vector by EcoRV (New England Biolabs) followed by heat inactivation and purification using a gel extraction kit (Qiagen) ...
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bioRxiv - Biophysics 2023Quote: ... 240 pmol of each primer (P1 and P2) and Phusion 2X mastermix with HF buffer (NEB, M0531S). The PCR mix was split into 100 μl aliquots and subjected to 35 cycles of 10 s 98 °C denaturation ...
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bioRxiv - Cell Biology 2023Quote: PCR with the teltail and P1 PCR primers was performed using Q5 PCR enzyme mix (NEB, #M0494) and the following conditions using 0.1 μl DNA-bound beads ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-MBP (NEB, E8032). Peroxidase and Alexa-fluor conjugated secondary antibodies were purchased from Jackson Laboratories and ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-myc (New England Biolabs), rabbit polyclonal anti- GFP (gift from Oliver Gruss ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The RNA was digested with nuclease P1 (Wako USA #145-08221) and dephosphorylated with Antarctic phosphatase (NEB #M0289S). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... primers P1 and P2 (Table S1) were used with the Q5 Hot Start High-Fidelity 2X Master Mix (NEB). To ensure coverage for each sample ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were brought up to 50μl and subsequently digested with 1μl of Nuclease P1 in 50mM sodium acetate buffer (New England Biolabs, M0660) for 2h at 45°C ...
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bioRxiv - Biochemistry 2022Quote: ... The RNA was first hydrolyzed overnight to nucleotide monophosphates using 300 U/ μg Nuclease P1 (NEB, 100,000 U/mL) at 37°C in 100 mM ammonium acetate (pH 5.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the IVT template RNA (0.01– 0.05 A260 units) was digested into nucleosides using 10 µg/ml nuclease P1 (New England Biolabs, M0660S), and 0.5 U/ml Bacterial Alkaline phosphatase (Takara 2120A ...