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Citations for New England Biolabs :
1 - 50 of 239 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... we dilute the stock DNA to a final concentration of 6 mg/mL with 1/10th volume of 10× CutSmart Buffer (0.5 M Potassium Acetate, 0.2 M Tris-acetate, 0.1 M Magnesium Acetate; New England BioLabs), 0.1% Tween (to prevent non-specific interactions) ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 DNA was extracted and amplified by Q5 High-Fidelity PCR (NEB, M0494S) using primers encompassing the sgRNA recognition site ...
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bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs), following the manufactures instructions.
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in 1 mL of NEB and centrifuged at 11,000rpm for 20 min on a sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The Nuclei pellet was then resuspended in 1 mL of NEB ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei-containing pellets were resuspended in 1 mL of NEB + 0,25 M sucrose and centrifuged at 20000xg for 20 min on sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The pellet was resuspended in 1 mL of NEB and formaldehyde to a final concentration of 1% ...
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bioRxiv - Microbiology 2020Quote: ... and M-MuLV reverse transcriptase (NEB) with buffer ...
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bioRxiv - Genomics 2022Quote: ... 200 μ M GTP (NEB, N0450S)).The run-on reaction was stopped by adding Trizol LS (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced ...
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bioRxiv - Genomics 2022Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced or used for molecular cloning ...
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bioRxiv - Genomics 2023Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced or used for molecular cloning ...
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bioRxiv - Neuroscience 2023Quote: ... M-MLV RT buffer (NEB; B0253S) 1X ...
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bioRxiv - Molecular Biology 2023Quote: ... M-MuLV Reverse Transcriptase (NEB, M0253L), RNase inhibitor (NEB ...
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bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs, USA) that specifically depletes cytoplasmic (5S ...
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bioRxiv - Microbiology 2021Quote: ... and M-MuLV reverse transcriptase (NEB M0253). For quantitative RT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... M-1 was digested with AflII (NEB) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 U M-MuLV RT (NEB) in RT Buffer (25 mM KCl ...
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bioRxiv - Molecular Biology 2020Quote: ... M-MuLV reverse transcriptase (New England Biolabs) and oligo-d(T ...
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bioRxiv - Systems Biology 2023Quote: ... and M-MuLV Reverse Transcriptase (NEB M0253). qPCR was performed in biological and technical triplicate using an Applied Biosystems QuantStudio 3 Real-Time PCR System with Fast SYBR Green Master Mix (Thermo Fisher 4385617) ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Microbiology 2021Quote: ... MeV-M BiFC constructs were made by exchanging NiV-M fused to VN173 or VC155 for MeV-M using the Gibson Assembly Cloning Kit (New England BioLabs). All mutant plasmids were generated using the Q5 site-directed mutagenesis kit using PAGE-purified mutagenesis primers designed using the online NEBase Changer primer design tool (New England BioLabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μL of M-MuLV reverse transcriptase (NEB), and 1 µL of murine RNase inhibitor (NEB ...
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bioRxiv - Genomics 2022Quote: ... 200 μ M ATP (New England Biolabs (NEB), N0450S) ...
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bioRxiv - Genomics 2022Quote: ... 200 μ M ATP (New England Biolabs (NEB), N0450S) ...
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bioRxiv - Plant Biology 2021Quote: ... M-TM2^2 was digested with BslI (NEB) for 3 hours at 55°C and M-2 was digested with XhoI (NEB ...
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bioRxiv - Microbiology 2021Quote: ... 1X M-MuLV Reverse Transcriptase buffer (NEB, M02553L) and 10 U/mL of M-MuLV Reverse Transcriptase (NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions ...
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bioRxiv - Microbiology 2021Quote: ... and M-MuLV Reverse Transcriptase (New England Biolabs) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and M-MuLV Reverse Transcriptase (cat# M0253S, NEB). qRT-PCR reactions were done using TaqMan® Fast Advanced Master Mix (cat# AB-4444557 ...
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bioRxiv - Bioengineering 2023Quote: XhoI restriction enzyme (NEB cat. no. R0146S/M)
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs). Regularly ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... HEK293 libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat. #E7770) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplifications of rbcL sequences were performed with the RbcL-M-F and RbcL-M-R primers using PCR Ready Mix (BioLabs, Israel), and 50ng DNA-template ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Immunology 2022Quote: Plasmids containing a cDNA encoding the full-length M segment from SNV (pWRG/SN-M(opt) 67 and pWRG/AND-M(opt2) 68 were used mutagenized using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat# E0554S). Primers were designed using the NEBaseChanger tool (https://nebasechanger.neb.com/) ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of genomic DNA were digested and loaded into each lane. Mouse (M. musculus and M. spretus) genomic DNA was digested with methylation-sensitive (HpyCH4IV, NEB cat. R0619L) or methylation-insensitive (ApoI ...
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bioRxiv - Neuroscience 2021Quote: ... m and f plasmids using HpaI and AccI (NEB). The first linker ...
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bioRxiv - Genomics 2022Quote: ... 0.7 µl T4 ligase (2 M U/ml, NEB), 250 ng DNA (adapters in File S1 ...
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bioRxiv - Plant Biology 2019Quote: ... and 20 units of M-MuLV (New England Biolabs) for 1 h at 42°C and then for 15 min at 70°C ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was incubated with M-MLV reverse transcriptase (NEB) with 10 mM dithiothreitol (DTT) ...
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bioRxiv - Neuroscience 2023Quote: ... 100 U of M-MLV Reverse Transcriptase (NEB; M0253S), M-MLV RT buffer (NEB ...
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bioRxiv - Bioengineering 2023Quote: HindIII-HF restriction enzyme (NEB cat. no. R3104T/M)
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in spheroblast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cell suspensions (1×106/100 μL) were treated with DNAse I (0.2 U/μL, NEB M0303S; NEB DNaseI buffer) or Benzonase (25 U/μL ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cell suspensions (1×106/100 μL) were treated with DNAse I (0.2 U/μL, NEB M0303S; NEB DNaseI buffer) or Benzonase (25 U/μL ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... RT-PCR was performed on cDNAs from murine Th17 or human HEK293 cells using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) with the standard thermocycling protocol ...
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bioRxiv - Molecular Biology 2022Quote: The HRAS minigene reporter was constructed by PCR amplifying a ∼900bp region spanning exon 4 to exon 6 from human genomic DNA isolated from HEK293 cells using Phusion high- fidelity DNA polymerase (NEB). The PCR fragment were inserted into pcDNA3.1(+ ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...