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Citations for New England Biolabs :
1 - 50 of 4040 citations for Leucine Rich Repeats And Immunoglobulin Like Domains Protein 1 LRIG1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... where the Emp24 transmembrane domain (residues 173-193) was replaced by 26 leucines using Gibson assembly (New England Biolabs). To construct pSP-FLAG-Cp ...
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bioRxiv - Microbiology 2021Quote: ... and Nico (λDE3) with reduced histidine-rich background proteins (NEB). The RNase I precursor contains a signal peptide (amino acid residues 1-23 ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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bioRxiv - Biochemistry 2019Quote: ... The CUG repeat construct was linearized with HindIII-HF (NEB). Restriction digestion reactions were performed according to the manufacturers’ recommendations ...
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bioRxiv - Biochemistry 2021Quote: pMAL-c4E vectors carrying in-frame fusions of the EFR cytoplasmic domain with the N-terminal maltose-binding protein (MBP) tag were transformed into Rosetta 2 cells (NEB) for recombinant protein expression ...
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bioRxiv - Developmental Biology 2019Quote: ... The recombinant DBINO domain containing protein (~80kDa) was purified using the amylose affinity column (New England Biolabs, Massachusetts, United States) and detected using the anti-MBP-HRP antibody (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR across the 70-bp repeats were carried out with LongAmp enzyme (NEB) according to standard protocols with primers PseudoF and PACR or RFPF and VSG221R.
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bioRxiv - Plant Biology 2020Quote: ... The amplification of the GC-rich region (primer 1) was performed with high fidelity Q5 polymerase (New England Biolabs Inc, Ipswich, MA) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Genetics 2020Quote: ... Repeats were amplified with high fidelity using the LongAmp Taq polymerase (New England Biolabs). Twenty to 50pg of DNA was amplified in a 25 µL reaction mix containing 2.5 units LongAmp Taq DNA polymerase ...
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bioRxiv - Biophysics 2020Quote: ... for the substrate-binding domain mutants and βamHI-AlwnI (NEB) for the transmembrane domain mutants (Appendix) ...
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bioRxiv - Immunology 2023Quote: Immunoglobulin libraries were generated from 1 μg of RNA using the NEBNext Immune Sequencing Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... according to the protocol for cloning DNA containing repeat elements (C3040, New England BioLabs Inc.). In both plasmids ...
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bioRxiv - Plant Biology 2023Quote: Mutants in the CG-1 domain were created using the Q5 site-directed mutagenesis kit (New England Biolabs, USA) as per manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: The kinase domain variant library was digested with PstI-HF (NEB) and NdeI-HF (NEB ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... Expansions too large for direct sequence (approximately >250 repeats) were digested with MspA1I (New England Biolabs) which ambiguously digests the PCR products containing either CGG or CTG interruptions in the CAG direction of the repeat tract ...
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bioRxiv - Microbiology 2020Quote: ... the C-terminal domain was removed by digestion with Endoproteinase LysC (NEB) at a 1:800 LysC:substrate ratio by mass for 1-2h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: DNMT3A PWWP domain was expressed in BL21(DE3) cells (New England Biolabs) using the same protocol as above ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: Purified DNA was amplified with human-or mouse-specific primers covering the RBM20 RS-domain mutation hotspot with Nextera-compatible adapters (Sup. Table 1) using Q5®Hot Start High-Fidelity 2X Master Mix (NEB). One microliter of a 1:100 dilution was used for a second PCR attaching sample-specific index barcodes (Nextera XT Index Kit v2 Set A ...
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bioRxiv - Neuroscience 2021Quote: ... C4 domain sequence was removed with the Q5 site-directed mutagenesis kit (NEB). Mouse prolyl 4-hydroxylase alpha- and beta-chain (P4HA1 and P4HB ...
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: Domain swap constructs were generated using Gibson assembly Master Mix (New England BioLabs). The coding sequences of RLP42 and RLP40 were cloned into pDONR207 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... CD45 catalytic domain DNA constructs were transformed into the BL21 (DE3) strain (NEB) Escherichia coli and plated on LB agar with ampicillin (100 mg/ml) ...
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bioRxiv - Plant Biology 2021Quote: ... The talIMAI1 central repeat region was then swapped into pSKX1_talFMAI1ΔAD using StuI and AatII (NEB, New England Biolabs) to generate pSKX1_talIMAI1ΔAD ...
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bioRxiv - Plant Biology 2021Quote: ... The talIMAI1 central repeat region was then swapped into pSKX1_talFMAI1ΔAD using StuI and AatII (NEB, New England Biolabs) to generate pSKX1_talIMAI1ΔAD ...
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bioRxiv - Cancer Biology 2023Quote: ... Oligonucleotide probe for telomere or Alu repeats was labeled with [32P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). The membrane was hybridized in Church hybridization buffer containing a 32P-labeled probe at 42°C overnight ...
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bioRxiv - Biophysics 2023Quote: ... into a pSGEM construct with five consecutive repeats of the myc tag (Lörinczi et al., 2015) using NcoI and HindIII (New England Biolabs).
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bioRxiv - Biochemistry 2021Quote: ... The MCS-intein-chitin binding domain of vector pTXB1 (New England Biolabs, Ipswitch, MA) was then inserted between sites NheI and Kpn2I ...
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bioRxiv - Immunology 2021Quote: ... CD4 domain deletion mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufactureŕs protocol ...
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bioRxiv - Biophysics 2022Quote: ... Deletion of N2B-us folding domain was accomplished by PCR and DPN1 (NEB, R0176S) reaction with subsequent KLD (NEB KLD Enzyme Mix ...
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bioRxiv - Plant Biology 2023Quote: ... The domain swap constructs were assembled using the NEBuilder HiFi DNA assembly kit (NEB). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... 28 novel RNF43 RING domain variants were generated using Q5 mutagenesis (New England Biolabs). All ZNRF3 and RNF43 expression plasmids were full-length sequence verified ...
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bioRxiv - Immunology 2024Quote: ... Immunoglobulin heavy and light chain cDNA was amplified using the Q5 High-Fidelity 2X Master mix (NEB) and pooled forward primers that bind the leader sequences of the variable regions and the reverse primers that bind the first exon of the mu and kappa constant regions ...
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bioRxiv - Cell Biology 2023Quote: ... The C-KSR-GS construct replacing the vector-based linker (LELKLRILQS) by a glycine-serine rich linker (GGSSGGGGA) was generated by site directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, USA) of the C-KSR-EGFP plasmid ...
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bioRxiv - Biochemistry 2022Quote: For crystallization the Ioc4 PWWP domain (aa 1-178; Ioc4PWWP) was cloned into a modified pMAL-c2X vector (New England Biolabs, Liu et al., 2001) in order to produce a fusion protein with an N-terminal maltose-binding protein (MBP) ...
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bioRxiv - Cell Biology 2020Quote: GST or GST-tagged SH3 domains were expressed in BL21 (DE3) E.coli (New England Biolabs). Cells were lysed by sonication in presence of lysozyme (Affymetrix) ...
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bioRxiv - Cell Biology 2023Quote: ... and mutant Pacer RH domain PCR product were mixed with Gibson Assembly master mix (NEB) in a 1:3:3 molar ratio prior to incubation at 50 °C for 15 minutes and transformation into XL10 E ...
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bioRxiv - Biophysics 2023Quote: ... The DNA of the SL5 domain was then digested using MlyI (New England Biolabs #R0610) and the DNA of the SL5-6 domains was then digested using BsaI (New England Biolabs #R0535 ...
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...
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bioRxiv - Molecular Biology 2021Quote: ... The SCR and its truncated version (tSCR or Ei) were cloned between the piggyBac transposon-specific inverted terminal repeat sequences (ITR) by linearizing the vector with BamHI (NEB, R3136) and NheI (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were then coupled to protein G beads (New England Biolabs). After beads were washed ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were detected using anti-MBP antibody (anti-MBP NEB e8032s) and HRP conjugated secondary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... extracting domain D2 from pDONR221/CD4 using primer pair P4 and performing a Gibson assembly (NEB) with the PCR products ...
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bioRxiv - Developmental Biology 2022Quote: ... The R462M point mutation in the PTP domain was introduced by Q5 site-directed mutagenesis (NEB). pCS2+ constructs were linearized using NotI and transcribed in vitro (mMessage machine SP6 ...
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bioRxiv - Biochemistry 2020Quote: ... Point mutations and domain deletions were generated using QuickChange site-directed mutagenesis with Q5 Polymerase (NEB). All plasmids were verified by DNA sequencing.
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bioRxiv - Immunology 2021Quote: ... Ig kappa or Ig lambda constant domains using the Quick Ligase cloning system (New England BioLabs) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the acquired Mlathy INR and INR-like sequences were confirmed by PCR using the Q5 Hot Start High-Fidelity kit (NEB), enzymatic clean-up using ExoSAP-IT™ (Thermo Fisher scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Immunoblotting was performed using HRP-conjugated anti-maltose binding protein (MBP) monoclonal antibody at 1:10000 dilution (New England Biolabs, #E8038) to verify equal expression levels of each construct.
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bioRxiv - Cell Biology 2022Quote: ... mTagBFP2 and Cas9-Avidin provided by Max Wilson into an expression vector bearing a CMV promoter and flanking PiggyBac transposase-compatible inverted terminal repeats using Gibson Assembly (New England BioLabs Inc., E2611L) according to supplier instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... an RNA-protein complex was formed with 1 µM Cas9 protein (New England Biolabs) and 1 µM gRNA pool (3 gRNAs in total ...