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Citations for New England Biolabs :
1 - 50 of 317 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... E.coli ECOR47 or E.coli dh5a F’ (NEB, C2992). For protein expression and purification ...
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bioRxiv - Biochemistry 2021Quote: LCB1 was synthesized and cloned into E.coli using a pET expression vector via Hi-Fi DNA assembly (NEB; E2621S) with a gBlock (ITD ...
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bioRxiv - Biochemistry 2020Quote: ... DH10B E.coli cells (NEB) were transformed with the resulting reaction via electroporation ...
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bioRxiv - Bioengineering 2021Quote: ... lacI and tac promoter from pMAL-c5X (New England Biolabs, Ipswich, MA), (4 ...
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bioRxiv - Bioengineering 2021Quote: ... (4) lacI and tac promoter from pMAL-c5X (New England Biolabs, Ipswich, MA). The modified replacement plasmid for pMut2 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples of E.coli cultures were harvested and resuspended in E.coli lysis reagent (NEB, US). Lysates and purified proteins were separated by 4-12% Bis-Tris SDS-PAGE (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... E.coli (NEB® 10-beta) containing both a Cas effector and gRNA plasmid (Table S3 ...
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bioRxiv - Genomics 2022Quote: ... amplified using E.coli Turbo (NEB), and extracted by NucleoBond midi-prep (Macherey Nagel) ...
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bioRxiv - Biophysics 2020Quote: ... 100 U E.coli RNAP (M0551S, NEB), 200 pM probe DNA ...
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bioRxiv - Genomics 2020Quote: ... containing 1.25U E.coli PolyA (NEB M0276S), 1.25X PolyA buffer (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... E.coli strain BL21(DE3) cells (NEB) were transformed and initially streaked to plates before a single colony was picked for a 10mls starter culture with selection and grown overnight ...
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bioRxiv - Microbiology 2023Quote: ... the allelic lacI-containing shuttle vector pMQ123 was first digested with BamHI (New England BioLabs) and the targeted small regulatory RNAs were amplified using Phusion polymerase (New England BioLabs ...
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bioRxiv - Biophysics 2023Quote: ... coli (MG1655) genome containing the rrnC operon was amplified by PCR (Phusion, NEB) using the following primers:
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bioRxiv - Genetics 2021Quote: ... Escherichia coli (E.coli) DNA polymerase I (NEB), RNase H (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... transformed in chemocompetent E.coli (New England Biolabs), isolated by miniprep and double-digested with restriction enzymes AgeI and EcoRI (both New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... 37.5 mU E.coli Poly(A) Polymerase (NEB), 50 mU T4 PNK (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... E.coli BL21 (D3) strains (New England Biolabs) were transformed with a T7-driven effector plasmid and an E.coli constitutive promoter J23119 to drive sgRNA expression ...
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bioRxiv - Molecular Biology 2022Quote: ... The mutated inserts were cloned into the wildtype operon vector digested with BstZ17I (NEB) using NEBuilder HiFi assembly and the full plasmids were sequence-verified.
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bioRxiv - Biochemistry 2021Quote: GST-PTP1B expression in BL21 competent E.coli (NEB) was induced by the addition of 0.2 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: Chemically competent BL21(DE3) E.coli (NEB, cat. C2527I) were transformed with pRSF bacterial expression plasmids of interest ...
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bioRxiv - Genomics 2022Quote: ... This was overexpressed in E.coli BL21 (DE3) (NEB) grown in M9 minimal media supplemented with vitamin (Centrum daily multivitamin) ...
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bioRxiv - Biophysics 2022Quote: ... BL21(DE3)-competent E.coli cells (New England Biolabs) were transformed with the plasmids and plated with Ampicillin (Amp) ...
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bioRxiv - Microbiology 2023Quote: ... The assembly was electroporated into 10B E.coli (NEB). The library construction of pUC57-NS5 was performed for each frame separately ...
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bioRxiv - Cell Biology 2023Quote: ... 2ul DNA polymerase I (E.coli, NEB cat. M0209S), 0.5ul RNase H (2.5 units) ...
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bioRxiv - Neuroscience 2023Quote: ... propagated in NEB Stable Competent E.coli (C3040, NEB) and isolated using endotoxin-free plasmid purification kit (Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... Candidate genes or operons were PCR amplified using Q5 high fidelity DNA polymerase (New England Biolabs) according to manufacturer’s instructions using primers (No ...
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bioRxiv - Microbiology 2020Quote: Constructs were transformed into E.coli T7 Express lysY (NEB) and isolated by ampicillin resistance ...
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bioRxiv - Systems Biology 2020Quote: ... The library was transformed into DH5α E.coli (NEB C2987H). The transformed bacteria were grown in 1L of semisolid LB plus 100 μg/ ml ampicillin (0.35% Seaprep Agarose ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transformed cells (New England Biolabs E.coli Turbo Cat.No C2984H) made competent using the protocol provided in [Additional file 1] were placed in the incubator overnight at 37° and colonies were screened for desired mutants using BsaI-HF®v2 (New England Biolabs Cat.No R3733S).
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bioRxiv - Genomics 2020Quote: ... into dam-/dcm-competent E.coli cells (NEB, Ipswich, MA). Unmethylated pUC19 was extracted using the Monarch Plasmid Miniprep Kit (NEB ...
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bioRxiv - Biophysics 2020Quote: ... SecYEG was obtained from E.coli BL21 (New England Biolabs) transformed with the arabinose dependent pBAD vector encoding cysteinless SecE ...
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bioRxiv - Biochemistry 2020Quote: ... E.coli BL21 (DE3) strain (New England BioLabs, Cat#C2527) was used for overexpression of xlGeminin and the N-terminal residues (1-170 ...
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bioRxiv - Biochemistry 2020Quote: ... E.coli T7 Express strain (New England BioLabs, Cat#C2566) was used for overexpression of LacI and M.HpaII ...
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bioRxiv - Genetics 2020Quote: ... coli competent cells (10-beta Competent E.coli, NEB C3019I) through an incubation of 30 minutes on ice and a heat shock of 30 seconds at 42°C ...
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bioRxiv - Biochemistry 2019Quote: Plasmids were used to transformed E.coli strain C2566 (NEB) protease-deficient and carrying pRARE plasmid (Novagen) ...
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bioRxiv - Cancer Biology 2022Quote: ... concentrated and transformed into 10b electrocompetent E.coli cells (NEB).
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bioRxiv - Synthetic Biology 2022Quote: ... The E.coli strain BL21(DE3) was obtained from NEB, all other strains were provided by the Gonzales lab.
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bioRxiv - Systems Biology 2023Quote: ... while a E.Coli 70s ribosome (NEB, cat nr P0763S) was used to estimate which fractions to use for ribosome XL-MS.
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bioRxiv - Microbiology 2020Quote: ... Plasmids carrying the dcas9/lacI cassette were recovered and propagated in the NEB 5-alpha F’ Iq strain (New England Biolabs). Cloning and/or propagation of plasmids containing the dcas9/lacI cassette in host E ...
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bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
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bioRxiv - Biophysics 2019Quote: ... and 1.5 U of E.coli DNA polymerase (New England Biolabs) and incubated for a further 20 minutes at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Ligation products were transformed into Stable Competent E.coli (NEB C3040). Transformed cells were cultured at 30°C overnight.
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bioRxiv - Genomics 2022Quote: ... The product was transformed into 10-beta Electrocompetent E.coli (NEB), by electroporation with 2.0kV ...
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bioRxiv - Genomics 2019Quote: ... Assembly products were electroporated into NEB Turbo Competent E.Coli (NEB) and then plated on ampicillin-containing medium ...
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bioRxiv - Bioengineering 2023Quote: ... and transformed into chemically competent SHuffle T7 Express E.coli (NEB). After bacterial liquid cultures were grown to 0.6 OD600 ...
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bioRxiv - Bioengineering 2023Quote: ... and transformed into chemically competent SHuffle T7 Express E.coli (NEB). After bacterial liquid cultures were grown to 0.6 OD600 ...
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bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
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bioRxiv - Synthetic Biology 2023Quote: ... (fhuA2 [lon] ompT gal (lambda DE3) [dcm] ΔhsdSlambda DE3 = lambda sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5) (New England Biolabs, C2527I) were used ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells and streaked onto Luria-Burtani (LB) plates containing carbenicillin. All-by-all repressor constructs (Fig. 5c) were cloned by digestion with BsiWI-HF (NEB) and BbsI (NEB) ...
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bioRxiv - Immunology 2021Quote: ... Plasmids were transformed in E.coli DH5α competent cells (NEB; Cat,# C2987I). All site-directed changes were confirmed by sequencing ...