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Citations for New England Biolabs :
1 - 50 of 599 citations for L Leucine N T Boc H2O 13C6 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... the 1:99 target:background sample was digested with FspEI (New England Biolabs) according to the manufacturers protocol (incubation at 37°C for 90 minute ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Bioengineering 2023Quote: SalI restriction enzyme (NEB cat. no. R0138S/T/L/M)
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bioRxiv - Bioengineering 2023Quote: T4 DNA Ligase (NEB cat. no. M0202S/T/L/M)
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bioRxiv - Bioengineering 2023Quote: PstI-HF restriction enzyme (NEB cat. no. R3140S/L/T/M)
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bioRxiv - Bioengineering 2023Quote: BamHI-HF restriction enzyme (NEB cat. no. R3136S/L/T/M)
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bioRxiv - Genomics 2024Quote: ... after which 68 µL RNase-free H2O was added with 10 µL DNase I Buffer and 2 µL DNase I (New England Biolabs, Ipswitch, MA). The resulting mix was incubated for 15m at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... and 15 uL of H2O) (New England Biolabs) for 1 hour at 37°C followed by DNase I (10 µL DNase I ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA was then digested using Dpn II (375 U DpnII (NEB, R0543 T/M/L) in 1× Dpn II buffer (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 41ul H2O and 1ul of Klenow fragment (NEB M0210L) were added to the annealed DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μL of 100 μM the forward and reverse DNA oligos were annealed in 47 μL annealing buffer (5 μl NEB buffer 3.1 and 42 μL H2O) by 5 min incubation at 95 °C and slow cool down in the heating block overnight ...
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bioRxiv - Genomics 2019Quote: ... 34 µl of H2O and 1 µl of PstI (NEB) were added and restriction digestion was performed at 37°C for 4 h ...
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bioRxiv - Microbiology 2022Quote: ... (2 µg enzyme in 100 µL H2O) (New England Biolabs) at 37 °C overnight ...
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bioRxiv - Genomics 2020Quote: ... 30 μL H2O) and 8 μL Large Klenow Fragment (NEB, M0210L) and 2 μL T4 PNK (NEB ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then reverse crosslinked and lysed by adding 10μL of 10X Lysis-T (250mM EDTA, 2M NaCl, 10% Triton X-100) and 4μL of proteinase K (NEB, P8107S) and incubating at 55°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... H2O to 50 μl and 0.5 μl Phusion polymerase (New England Biolabs). For the PCR conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... and nuclease-free H2O up to 20 μL (New England Biolabs, USA). The reaction was performed in 30 cycles of 37 °C for 5 min followed by 16 °C for 5 min and finalized by incubation at 60 °C for 5 min ...
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bioRxiv - Biochemistry 2021Quote: The α-(1,2)-Galf-containing N-linked glycans were released from 1 mg Transglucosidase L “Amano” (Amano) by using PNGase F (New England Biolabs) under denaturing conditions ...
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bioRxiv - Cell Biology 2020Quote: ... where the Emp24 transmembrane domain (residues 173-193) was replaced by 26 leucines using Gibson assembly (New England Biolabs). To construct pSP-FLAG-Cp ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with 7.1 μl H2O and 0.9 μl NEB 4 buffer (NEB). After incubating for 10 min at 96°C ...
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bioRxiv - Genetics 2019Quote: ... and 2.0 units of the CspCI enzyme ((N)10-11CAA(N)5GTGG(N)12-13) (New England Biolabs). The library preparation was carried out according to the protocol proposed by Osorio-Guarín et al ...
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bioRxiv - Bioengineering 2021Quote: ... H2O up to 100 μL) was performed with phi29 DNA polymerase (New England Biolabs) at 30 °C for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 20µL sample + 6µL H2O + 1µL proteinase K (200µg/mL) (New England BioLabs, Ipswich, MA). 3 ...
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bioRxiv - Biochemistry 2023Quote: ... The dried pellet is then resuspended in 40µl H2O and digested with BsrGI (NEB) in a total volume of 50 μl for 2 h at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK) (NEB, M0201). The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938) ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA was digested with 2.0 units of the BsaXI enzyme ((N)9AC(N)5CTCC(N)10) (New England Biolabs) and 2.0 units of the CspCI enzyme ((N)10-11CAA(N)5GTGG(N)12-13 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4.375 U RNase T (NEB), 1.875 U XRN-1 (NEB) ...
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bioRxiv - Genetics 2019Quote: ... After the cells were resuspended in H2O and CutSmart® Buffer (New England Biolabs, Cat#B7204) and treated with 0.3% SDS and 2.5% Triton X100 at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were washed in PBS-T and blocked in PBS-T + 2% BSA (NEB, B9000) + 5% donkey/goat serum (Jackson Immunoresearch/Dianova ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Stereo-seq chip was washed with NF-H2O supplemented with 0.05 U/ul RNase inhibitor (NEB, M0314L). Six sections from the anterior half endostyle were mounted onto a single Stereo-seq chip with 3-minute incubation on a Thermocycler Adaptor at 37℃ ...
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bioRxiv - Genomics 2023Quote: ... PCR master mix (64 µL of H2O, 100 µL of Q5 high-fidelity DNA polymerase (NEB #M0544S), 8 µL of i5 primer ...
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bioRxiv - Bioengineering 2019Quote: ... Poly-d(T) beads (NEB #S1419S) were washed twice with 100 μL wash and bind buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Genomics 2020Quote: ... oligo d(T)23VN primer (NEB) was annealed to template RNA ...
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bioRxiv - Genetics 2022Quote: ... and exonuclease T (NEB, catalog # M0625). Blunt ends were A-tailed and capped with END-seq adaptor 1 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... and Oligod(T)23VN (NEB, S1327) following the manufacturer’s instruction for SuperScript First-Strand Synthesis System (Thermo Fisher).
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bioRxiv - Molecular Biology 2024Quote: ... and exonuclease T (NEB, catalog # M0625). Blunt ends were A-tailed and capped with END-seq adaptor 1 ...
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bioRxiv - Microbiology 2020Quote: ... Enzymatics) and end repaired Silane purified cDNA eluted in in 32 μl of RNase free H2O (1x NEB buffer 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified DNA was eluted in 17 μL H2O and treated with exonuclease (New England Biolabs, ordering info missing). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... (1.5 μM x 2.5 μL in H2O) followed by addition of 4.5 μL NEBNext Ultra II Pol master mix (NEB). The reaction mixture was denatured at 98 °C for 1 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... One acceptor and one or more donors were mixed with a ratio of 1:1.1 in distilled H2O with 0.5 ul (7.5 U) of CRE recombinase (NEB # M0298M) and 1 ul Cre buffer (provided with CRE recombinase ...
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bioRxiv - Genomics 2021Quote: ... 50 µl of ULR-Phusion mix (1.2 µl ULR Ladder, 28.8 µl H2O, 30 µl Phusion PCR mastermix [New England Biolabs] and the samples were mixed with 147.5 µl ProNex chemistry ...
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bioRxiv - Genetics 2023Quote: ... (1.33x Gibson-Assembly Master-Mix [for 25 reactions]: 100 μL 5x Isothermal Reaction-Mix, 215.32 μL H2O, 0.2 μL T5 Exonuclease [10 U/μL NEB #M0363] ...
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bioRxiv - Microbiology 2021Quote: ... 50 pmol Oligo d(T)23 (NEB) and 10 pmol Deoxynucleotide Triphosphate Mix (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... Magnetic oligo-d(T) beads (NEB, S1419S) were equilibrated in NLB and added to the enrichments ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and de-N-glycosylated using 500 U N-glycosidase F (PNGase F, New England Biolabs) for 12 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Single cells of two untreated CeD patients were sorted into 96-well plates containing 2 µl of scRNA-seq catch buffer (0.2% vol/vol Triton X-100 [Sigma] in H2O with 2 U/μl RNase inhibitor [New England Biolabs]) per well using a FACSAriaIII instrument (BD) ...
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bioRxiv - Neuroscience 2023Quote: ... N-glycans were released using N-glycanase PNGase F (1239U/ml, New England BioLabs, Inc. cat no. P0709L) and were fluorescently labelled with 2-aminobenzamide (2-AB ...
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bioRxiv - Bioengineering 2022Quote: ... or 1 μg Asp-N (NEB, England) for overnight digestion respectively ...
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bioRxiv - Immunology 2020Quote: ... deglycosylated with N-glycanase (New England Biolabs), and digested overnight with LysC protease (ThermoFisher scientific) ...