Labshake search
Citations for New England Biolabs :
1 - 50 of 254 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... after which 68 µL RNase-free H2O was added with 10 µL DNase I Buffer and 2 µL DNase I (New England Biolabs, Ipswitch, MA). The resulting mix was incubated for 15m at 37°C ...
-
bioRxiv - Bioengineering 2020Quote: ... and 15 uL of H2O) (New England Biolabs) for 1 hour at 37°C followed by DNase I (10 µL DNase I ...
-
bioRxiv - Molecular Biology 2022Quote: ... 41ul H2O and 1ul of Klenow fragment (NEB M0210L) were added to the annealed DNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μL of 100 μM the forward and reverse DNA oligos were annealed in 47 μL annealing buffer (5 μl NEB buffer 3.1 and 42 μL H2O) by 5 min incubation at 95 °C and slow cool down in the heating block overnight ...
-
bioRxiv - Genomics 2019Quote: ... 34 µl of H2O and 1 µl of PstI (NEB) were added and restriction digestion was performed at 37°C for 4 h ...
-
bioRxiv - Microbiology 2022Quote: ... (2 µg enzyme in 100 µL H2O) (New England Biolabs) at 37 °C overnight ...
-
bioRxiv - Genomics 2020Quote: ... 30 μL H2O) and 8 μL Large Klenow Fragment (NEB, M0210L) and 2 μL T4 PNK (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... H2O to 50 μl and 0.5 μl Phusion polymerase (New England Biolabs). For the PCR conditions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and nuclease-free H2O up to 20 μL (New England Biolabs, USA). The reaction was performed in 30 cycles of 37 °C for 5 min followed by 16 °C for 5 min and finalized by incubation at 60 °C for 5 min ...
-
bioRxiv - Genomics 2022Quote: ... the 1:99 target:background sample was digested with FspEI (New England Biolabs) according to the manufacturers protocol (incubation at 37°C for 90 minute ...
-
bioRxiv - Molecular Biology 2022Quote: ... was mixed with 7.1 μl H2O and 0.9 μl NEB 4 buffer (NEB). After incubating for 10 min at 96°C ...
-
bioRxiv - Bioengineering 2021Quote: ... H2O up to 100 μL) was performed with phi29 DNA polymerase (New England Biolabs) at 30 °C for 20 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... 20µL sample + 6µL H2O + 1µL proteinase K (200µg/mL) (New England BioLabs, Ipswich, MA). 3 ...
-
bioRxiv - Biochemistry 2023Quote: ... The dried pellet is then resuspended in 40µl H2O and digested with BsrGI (NEB) in a total volume of 50 μl for 2 h at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK) (NEB, M0201). The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938) ...
-
bioRxiv - Genetics 2019Quote: ... After the cells were resuspended in H2O and CutSmart® Buffer (New England Biolabs, Cat#B7204) and treated with 0.3% SDS and 2.5% Triton X100 at 37°C for 1 hour ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Stereo-seq chip was washed with NF-H2O supplemented with 0.05 U/ul RNase inhibitor (NEB, M0314L). Six sections from the anterior half endostyle were mounted onto a single Stereo-seq chip with 3-minute incubation on a Thermocycler Adaptor at 37℃ ...
-
bioRxiv - Genomics 2023Quote: ... PCR master mix (64 µL of H2O, 100 µL of Q5 high-fidelity DNA polymerase (NEB #M0544S), 8 µL of i5 primer ...
-
bioRxiv - Microbiology 2020Quote: ... Enzymatics) and end repaired Silane purified cDNA eluted in in 32 μl of RNase free H2O (1x NEB buffer 2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Purified DNA was eluted in 17 μL H2O and treated with exonuclease (New England Biolabs, ordering info missing). Subsequently ...
-
bioRxiv - Biochemistry 2020Quote: ... (1.5 μM x 2.5 μL in H2O) followed by addition of 4.5 μL NEBNext Ultra II Pol master mix (NEB). The reaction mixture was denatured at 98 °C for 1 min ...
-
bioRxiv - Synthetic Biology 2020Quote: ... One acceptor and one or more donors were mixed with a ratio of 1:1.1 in distilled H2O with 0.5 ul (7.5 U) of CRE recombinase (NEB # M0298M) and 1 ul Cre buffer (provided with CRE recombinase ...
-
bioRxiv - Genomics 2021Quote: ... 50 µl of ULR-Phusion mix (1.2 µl ULR Ladder, 28.8 µl H2O, 30 µl Phusion PCR mastermix [New England Biolabs] and the samples were mixed with 147.5 µl ProNex chemistry ...
-
bioRxiv - Genetics 2023Quote: ... (1.33x Gibson-Assembly Master-Mix [for 25 reactions]: 100 μL 5x Isothermal Reaction-Mix, 215.32 μL H2O, 0.2 μL T5 Exonuclease [10 U/μL NEB #M0363] ...
-
bioRxiv - Immunology 2023Quote: ... Single cells of two untreated CeD patients were sorted into 96-well plates containing 2 µl of scRNA-seq catch buffer (0.2% vol/vol Triton X-100 [Sigma] in H2O with 2 U/μl RNase inhibitor [New England Biolabs]) per well using a FACSAriaIII instrument (BD) ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was purified with Silane beads and eluted in 52 μl of RNase free H2O as input for end repair reaction using End Repair Module (NEB) following manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2019Quote: ... up to 500 ng of gDNA was incubated in 10 µL H2O with 1x CutSmart buffer and 0.5 U rSAP (New England BioLabs #M0371L) for 1 hour at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Beads were resuspended in H2O used for library preparation with the NEBNext Ultra II Library Prep kit for Illumina (NEB). Beads were then washed 4 times using Tween wash buffer (5 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... gRNAs were annealed in (1 μl Forward primer, 1 μl Reverse primer, 5 μl Buffer 4 NEB, 43 μl H2O) by heating at 98 °C for 5 min and allowing the tubes to cool down to room temperature in the thermoblock (∼3h) ...
-
bioRxiv - Microbiology 2020Quote: ... In the meantime, we prepared 150 μl of T4 PNK mix (120 μl H2O, 20 μl 10x T4 PNK buffer (NEB), 1 μl Murine RNase Inhibitor (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... before resuspending the beads in 25 μl RNA ligation mix (9 μl H2O, 3 μl 10x T4 RNA ligase buffer (NEB), 0.3 μl 0.1M ATP ...
-
bioRxiv - Genomics 2021Quote: ... The PCR mix (8 µl H2O, 2 µl primer mix P5Solexa/P3Solexa, 10 µM each, 20 µl Phusion HF Mix [New England Biolabs]) was added to 10 µl cDNA ...
-
bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer’s instructions (with an elution in 30 μL sterile filtered MilliQ H2O) and restriction digest with suitable restriction enzymes (New England Biolabs) of about 250 ng plasmid DNA for 3 h ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA pellets were suspended in 50 μl of H2O and 10 μg of DNA was digested by EcoRI restriction endonuclease (NEB) at 37 °C for 1 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... containing the sgRNA sequences were resuspended at 100 nM in H2O and amplified by PCR using HF Phusion polymerase (New England Biolabs). After verifying amplification on a 10% acrylamide gel ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The coding and reverse oligonucleotides were mixed (6 µL H2O, 1 µL T4 Ligase Buffer, 1 µL T4 PNK (10 U/µL; NEB), 1 µL of each 100 µM oligonucleotide ...
-
bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
-
bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
-
bioRxiv - Bioengineering 2020Quote: ... Esp31 (NEB R0734S/L), and T7 DNA Ligase (NEB M0318S/L ...
-
bioRxiv - Bioengineering 2020Quote: ... Esp31 (NEB R0734S/L), and T7 DNA Ligase (M0318S/L) ...
-
bioRxiv - Bioengineering 2020Quote: ... Esp31 (NEB R0734S/L), and T7 DNA Ligase (M0318S/L ...
-
bioRxiv - Microbiology 2024Quote: ... Murine (NEB, #M0314S/L) and nuclease-free water at 37°C for 30 minutes ...
-
bioRxiv - Genetics 2021Quote: ... the RNP complex was assembled by incubating 9 μL of guide RNA with 3 μL of nuclease in 12 μL of nuclease-free H2O with 3 μL of 10x Cas9 reaction buffer (New England Biolabs, #B0386) at 37 °C for 15 minutes ...
-
bioRxiv - Systems Biology 2023Quote: ... We resuspended each oligo to 25 uM in NF-H2O and performed phosphorylation and annealing of each oligo pair by combining the oligos with 1X T4 ligase buffer (NEB #B0202S) and polynucleotide kinase (NEB #M0201S ...
-
bioRxiv - Bioengineering 2023Quote: ... We resuspended each oligo to 25uM in NF-H2O and performed phosphorylation and annealing of each oligo pair by combining the oligos with 1X T4 ligase buffer (NEB #B0202S) and polynucleotide kinase (NEB #M0201S ...
-
bioRxiv - Developmental Biology 2023Quote: ... 0.5XSSC in H2O) on a light table for 1 hour and treated with 2 μg/mL Protease K (NEB, Cat PB107S) in PBS containing 0.3% Triton-X for 10 minutes followed by post-fixation in 4% PFA for 10 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... Enzyme restriction with DpnII was performed in 30 µl reaction mix (1 µl DpnII, 3 µl DpnII buffer, 10 µl purified DNA and 16 µl H2O; New England Biolabs, Ipswich, MA) and incubation at 37°C for 2h ...
-
bioRxiv - Microbiology 2020Quote: ... 1.5μL of 100uM the forward and reverse DNA oligos were annealed in 47μL annealing buffer (5ul NEB buffer 3.1 and 42μL H2O) by 5min incubation at 95C° and slow cooldown in the heating block overnight ...
-
bioRxiv - Genomics 2021Quote: The beads were magnetically separated and 4 µl of hot PNK mix (0.2 µl PNK [New England Biolabs], 0.4 µl 32P-γ-ATP, 0.4 µl 10x PNK buffer [New England Biolabs], 3 µl H2O) was added and incubated for 5 min at 37°C in a thermomixer at 1,100 rpm ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.1 g/L BSA (NEB), 1x EvaGreen Dye (Biotium ...