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Citations for New England Biolabs :
1 - 50 of 214 citations for Interleukin 35 IL 35 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and 35 µl rSAP (NEB) were added and sample was incubated for at least 8 h at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... was first generated by removing BsmBI restriction sites on pU6-sgRNA-EF1Alpha-puro-T2A-BFP (Addgene #60955)35 through Golden Gate Assembly (NEB E1602S). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... 35 µL of 10X NEB2.1 buffer (New England Biolabs) was added to each sample ...
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bioRxiv - Genomics 2023Quote: ... then dissolved in a 35 μL elution buffer (EB, NEB) heated to 50°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Reactions were cycled 35 times with annealing temperatures calculated by NEB Tm calculator prior to loading on 2% agarose gels ...
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bioRxiv - Molecular Biology 2021Quote: ... 35 units of mung bean nuclease (New England Bio Labs (NEB)) and 0.02 mg/ml RNase A (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... using anchored oligo(dT)35 and random nonamers (New England Biolabs, USA), as per the protocol recommended by the manufacturer.
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bioRxiv - Biochemistry 2023Quote: ... 500 µL supernatant was applied to 35 µL amylose resin (New England Biolabs) or glutathione resin (Cytiva ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was used for PCR amplified for 35 cycles using Taq polymerase (NEB M0273) and respective primers (Table 1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples 35 and 36 were prepared using the NEBNExt FFPE DNA Repair MIX (NEB). DNA fragment shorter than 20Kb were removed for sample 35 and 36 ...
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bioRxiv - Cell Biology 2023Quote: ... transferred to a 1.5ml tube and incubated with 35 μM benzylguanine conjugated fluorophore (NEB, S9136S) for 2 hours before being returned to the column ...
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bioRxiv - Cell Biology 2024Quote: ... Serine-35 mutations were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers are listed in the supplemental methods.
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bioRxiv - Cancer Biology 2023Quote: ... Standard cloning was performed using PCR (35 μL ddH2O, 10 μL 5x HF Phusion buffer (NEB), 1 μL of 10 mM mixed dNTPs ...
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bioRxiv - Cell Biology 2019Quote: ... at 30°C for 1 hour in a total volume of 50μl PMP phosphatase buffer (50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM DTT, 0.01% Brij 35, 1 mM MnCl2; New England Biolabs).
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bioRxiv - Cell Biology 2022Quote: Size markers 19 and 35 nucleotides long were dephosphorylated using 0.5 U/ μl CIP alkaline phosphatase (NEB M0290L) by incubating at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... beads were equilibrated in 1x PMP buffer (50 mM HEPES, 100 mM NaCl, 2 mM DTT, 0.01% Brij 35, pH 7.5; New England Biolabs). Kinase reactions were performed with 1 μL commercial CK1δ (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 35 ng/μL template DNA was incubated with 1.5 U/μL murine RNase inhibitor (New England Biolabs, USA), 0.2 μM inorganic pyrophosphatase ...
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bioRxiv - Molecular Biology 2024Quote: ... and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated by centrifugation at 90,000 g for 35 min and the solubilized complex was incubated with amylose resin (NEB) for 2.5 h at 4 C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated by centrifugation at 90,000 g for 35 min and the solubilized complex was incubated with amylose resin (NEB) for 2.5 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Insoluble material was pelleted at 119,000g for 35 minutes and the supernatant was bound to amylose resin (New England Biolabs). The resin was washed with lysis buffer and eluted with lysis buffer plus 10 mM maltose ...
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bioRxiv - Molecular Biology 2023Quote: ... Re-joined ends were amplified by 35 cycles of PCR from 0.8 µl template using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and primers NOSprom-nested-F2 and TagRFP-nested-R2 (Table S1 ...
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bioRxiv - Microbiology 2024Quote: ... the sequence encoding amino acid residues 1 to 35 of the N-terminal end of BVG96_RS17270 (89) was PCR amplified using Q5 polymerase (NEB) and cloned via NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Microbiology 2021Quote: The chimeric CrPV-aNCV infectious clone was derived from the full-length CrPV infectious clone (pCrPV-3; Accession KP974707) (35) using Gibson assembly (New England BioLabs), per the manufacturer's instructions ...
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bioRxiv - Plant Biology 2019Quote: ... and cloned either alone or under of the 35S promoter together with GFP and mScarlet-i into pPLV03 or pGEX5x3 using Gibson Assembly (NEB). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: Deglycosylation of S protein from the lysate of HEK293 cells transfected with pTwist-EF1a-nCoV-2019-S-2xStrep plasmid was performed as described previously [35] using PNGase F (New England Biolabs).
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bioRxiv - Cell Biology 2021Quote: ... coding sequence was amplified from Arabidopsis cDNA and assembled into a pGreen0179-35S-spFLA11-His-YFP vector using NEBuilder HiFi DNA Assembly kit (NEW ENGLAND Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... A volume of cDNA corresponding to 2.5 ng of the input RNA was subjected to 35 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
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bioRxiv - Synthetic Biology 2019Quote: ... two oligonucleotides were designed with an overlap of 35 bp containing the T7 promoter sequence and amplified using Q5 High-Fidelity DNA polymerase (NEB) according to the suppliers instructions ...
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bioRxiv - Biochemistry 2019Quote: ... expression constructs containing WT or mutant full-length human KAP1 preceded by a triple FLAG tag and linker sequence MDYKDHDGDYKDHDIDYKDDDDKGSGG were assembled in pLEXm (35) with the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). The following plasmids were used ...
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bioRxiv - Synthetic Biology 2021Quote: ... [35] obtained from Benenson lab at ETH Zurich was digested with 30 U of SalI-HF (New England Biolabs (NEB), R3138S ...
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bioRxiv - Genetics 2020Quote: ... The purified DNA fragment (40 ng) was ligated into pICSLUS0003OD (35S USER vector) with the USER enzyme (New England Biolabs). After verifying the presence of the insert by colony PCR ...
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bioRxiv - Genetics 2019Quote: ... The affl-2 driver strain was constructed by injecting 25 ng/µl of pSJW003 along with 40 ng/µl of Punc-122::rfp and 35 ng/µl of 1 kb DNA ladder (NEB) into the GFP effector strain syIs300 ...
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bioRxiv - Plant Biology 2022Quote: All ObANS genes were inserted into the pBI121 plasmid under the control of the constitutive Cauliflower mosaic virus 35S promoter using the SacI and XbaI restriction enzymes (Thermo-scientific) and the NEBuilder ligation system (NEB). The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 using electrotransformation and positive colonies were selected with kanamycin ...
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bioRxiv - Genomics 2023Quote: ... was cloned between residues 34 and 35 with primers ag424 and ag425 using inverse PCR with Q5 polymerase (New England Biolabs, NEB).23 The sequences of all primers used in this study are presented in Table S2 ...
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bioRxiv - Plant Biology 2023Quote: ... and enhancer fragment combinations were cloned upstream of the 35S minimal promoter by Golden Gate cloning using BsaI-HFv2 (NEB). The resulting libraries were bottlenecked to yield 10–20 barcodes per enhancer.
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bioRxiv - Plant Biology 2023Quote: ... and rbcS-E9 enhancers and the synthetic enhancers syn1–11 were cloned upstream of the 35S minimal promoter in pDL by Golden Gate cloning using BsaI-HFv2 (NEB). The synthetic enhancers were ordered as synthesized DNA fragments.
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bioRxiv - Synthetic Biology 2021Quote: ... Bi-directional precursor construct (pZ073) [35] obtained from Benenson lab at ETH Zurich was digested with 30 U of SalI-HF (New England Biolabs (NEB), R3138S ...
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bioRxiv - Genomics 2022Quote: ... 1500 calcein-stained cells in 5 μL of were added to 35 μL of barcoded hydrogel templates with 29 U/mL Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Molecular Biology 2023Quote: ... full length CDS of TaSnRK1α gene was amplified using the wheat cDNA and cloned into the pCAMBIA1302 vector under the constitutive promoter 35S using the Gibson assembly cloning kit (Gibson Assembly® Master Mix, NEB) with the help of restriction enzyme NcoI ...
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bioRxiv - Genomics 2023Quote: ... was cloned between residues 34 and 35 with primers ag424 and ag425 using inverse PCR with Q5 polymerase (New England Biolabs, NEB).23 The sequences of all primers used in this study are presented in Table S2 ...
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bioRxiv - Plant Biology 2023Quote: The coding region of NIa-Pro protein was cloned from TuMV into the binary vector pMDC32 downstream of the 35S promoter as well in pSITE vector using Gibson assembly (New England Biolabs, USA)(Gibson et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... The rbpms2bsa9329 surrounding genomic region was amplified for 35 cycles with an annealing temperature of 60°C and the mutant allele was digested with MboII (New England Biolabs, R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: ... Full-length cDNA sequences were obtained in 35 cycles of PCR reactions with Phusion DNA polymerase (New England Biolabs, Ipswich, USA; MO531L) and specific primers designed against the sequence of the phylogenetically characterized white seabass β-NHE obtained from the combined gill and RBC transcriptome (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... full length CDS of TaSnRK1α was cloned into plant expression vector pCAMBIA1302 under the constitutive promoter CaMV:35S with the help of restriction site NcoI on both ends using Gibson assembly® master mix (NEB, USA). Primer details are provided in Supplementary Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei of 106 cells plated on a 35 mm dish were isolated by addition of nuclei extraction buffer (NEB, 20 mM Hepes (KOH) pH 7.9 ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... RT-PCR was performed on cDNAs from murine Th17 or human HEK293 cells using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) with the standard thermocycling protocol ...
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bioRxiv - Molecular Biology 2022Quote: The HRAS minigene reporter was constructed by PCR amplifying a ∼900bp region spanning exon 4 to exon 6 from human genomic DNA isolated from HEK293 cells using Phusion high- fidelity DNA polymerase (NEB). The PCR fragment were inserted into pcDNA3.1(+ ...
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bioRxiv - Microbiology 2019Quote: ... The 16S rRNA gene was amplified using 27F 34 and 534R 35 modified for Illumina sequencing following standard protocols Q5® High-Fidelity DNA Polymerase (New England Biolabs, Inc., Ipswich, MA, USA). Following amplification ...
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bioRxiv - Neuroscience 2022Quote: The three NMDAR fusion protein constructs were subcloned into pCSE2.7-mIgG2a-Fc-XP (N1-Fc and N2B-Fc) or pCSE2.8-mIgG2a-Fc-Xp (N1-N2B-Fc) using NcoI/NotI (New England Biolabs, Frankfurt, Germany) for mammalian production in Expi293F cells ...