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Citations for New England Biolabs :
1 - 50 of 171 citations for IL 19 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Barcodes were amplified by 19 cycles of PCR using Phusion polymerase (NEB) and primers that add Illumina adapter sequences and an 8 bp identifier sequence used to distinguish libraries and time points ...
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bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... re-suspended with 19 μl reaction buffer contain 2ul 10x T4 DNA ligase buffer (NEB), 1 μl USER Enzyme (NEB ...
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bioRxiv - Genetics 2019Quote: ... The 19 base-sgRNA was cloned into pDD162 using a Q5 mutagenesis kit (New England Biolabs), as described by 48 ...
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bioRxiv - Cancer Biology 2021Quote: ... a barcoded RNA adapter (RiL 19) was ligated to the 3’ end using T4 RNA ligase (NEB). The 3’ adaptor ligated RNA was again cleaned up as mentioned above ...
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bioRxiv - Plant Biology 2021Quote: ... DNA fragments were PCR-amplified for 19 cycles using the NEB 2X Taq MasterMix (NEB, MA, USA) and 35 ng of starting DNA in an Eppendorf Mastercycler ep gradient S (Eppendorf ...
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bioRxiv - Genomics 2021Quote: ... PCR reaction according to the manufacturer’s protocol (annealing temperature: 60°C; elongation time: 15sec, 19 cycles) (NEB). Of this reaction ...
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bioRxiv - Genomics 2023Quote: ... 1 μl of Hia5 MTase (200 U)19 and 1.5 μl 32 mM S-adenosylmethionine (NEB B9003S) (0.8 mM final concentration ...
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bioRxiv - Cell Biology 2022Quote: Size markers 19 and 35 nucleotides long were dephosphorylated using 0.5 U/ μl CIP alkaline phosphatase (NEB M0290L) by incubating at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... using a golden gate assembly strategy (19) and a type-II restriction SapI enzyme (New England Biolabs, R0569). A similar cloning strategy was used to build vectors bearing a JcDV viral genomes with fragments of cellular genes (~160 nucleotides ...
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bioRxiv - Cancer Biology 2021Quote: ... and succinyl-lysine (PTM Biolabs, Chicago, IL, USA) were used to capture SIRT1-SIRT7 proteins overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Barhl1a riboprobe was synthesized as previously reporter 19 and gfp riboprobe was synthesized from a linearized pCS2:GFP plasmid with NotI (Fermentas or New England Biolabs) and transcribed with Sp6 (mMessage mMachine Sp6 ...
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bioRxiv - Microbiology 2020Quote: The vector backbone was amplified from pMo130 using primers JMC203-JMC204 (Supplementary Table 19) with Q5 DNA polymerase (New England Biolabs), cleaned up and treated with DpnI (New England Biolabs) ...
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bioRxiv - Biochemistry 2019Quote: ... fused to the 3’ end of the nucleotide sequence encoding full length Ypt7 was amplified by PCR from pET-19 Ypt7-tm (a kind gift from C Ungermann) with the Phusion high-fidelity DNA polymerase (NEB). The DNA fragment was cloned into BamHI and SalI digested pMBP-parallel1 vector (Sheffield et al. ...
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bioRxiv - Immunology 2022Quote: ... and nCoV_IP4-14084Probe(+) TCATACAAACCACGCCAGG [5’]Hex [3’]BHQ-1 19) and the Luna Universal Probe One-Step RT-qPCR Kit (NEB). Serial dilutions of a titrated viral stock were analyzed simultaneously to express viral loads as eqPFU per gram of tissue.
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
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bioRxiv - Biochemistry 2022Quote: ... 100 ng of each gel-purified PCR products (total of 19) were mixed and digested with BsmBI restriction enzyme (NEB) for 2 h at 55 °C ...
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bioRxiv - Microbiology 2023Quote: ... biwaensis Akk2750 (100% sequence match to CSUN-19) (38) with Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and incorporating a C-terminal HA epitope tag ...
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bioRxiv - Molecular Biology 2024Quote: ... Both ends of the 19-mer of the 200 bp 601 DNA were labeled with biotin by Klenow fragment (NEB) with biotin-14-dATP (58) ...
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bioRxiv - Developmental Biology 2021Quote: ... Adaptor ligated RNAs 48-58nt long (corresponding to 19-29nt long input RNAs) were extracted and ligated to the 5’ adaptor using T4 RNA Ligase 1 (NEB, M0204). A total of ten variable nucleotides (unique molecular identifiers ...
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bioRxiv - Genetics 2020Quote: ... 50 pg of repaired and adapter-ligated products were amplified for 19 cycles with the NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544) and dual-indexed with custom IDT Ultramer primers ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Microbiology 2019Quote: ... falciparum and human histones (New England Biolabs) (6µg) ...
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bioRxiv - Microbiology 2021Quote: ... 40 U RNase Inhibitor Human Placenta (NEB), 50 pmol Oligo d(T)23 (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... Human CK2 was obtained from NEB (P6010S). IE2-NTD was phosphorylated in buffer provided by the manufacturer which contains 50mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... pFastBac dual vector containing the sequence for the N-terminally 6xHis-tagged human Naa15 and truncated human Naa10 (residues 1-160) sequences was digested using KpnI-HF (NEB) and XmaI (NEB ...
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bioRxiv - Biochemistry 2023Quote: Human HA-DHFR was subcloned from human cDNA to pcDNA4/TO by PCR using Q5 High Fidelity 2X mastermix (NEB). Human FPGS-FLAG and GGH-FLAG were subcloned from cDNA clones MHS6278-202755815 (Horizon Discovery ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... MARCH2 chimeras containing the TM domains from either human MARCH4 or human transferrin receptor (TR) were generated using the NEBuilder HiFi DNA assembly kit (New England Biolabs) and the primers listed in Supplemental Table S2 ...
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bioRxiv - Biophysics 2020Quote: ... from human origin were all purchased from NEB. The H2A/H2B and H3.1/H4 were mixed in 2(H2A/H2B)2:1(H3/H4)4 molar ratio to form octamers.
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant human histone H4 (New England Biolabs # M2504S) was used as a substrate ...
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bioRxiv - Immunology 2022Quote: ... with short homologies for Gibson assembly and cloned into human IgG1 or human IgL2 expression vectors using the NEB Hifi DNA Assembly mix (NEB, Cat#E2621L). Plasmid sequences were verified by Sanger sequencing (Genewiz).
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... Human plasma was treated with PNGase F (NEB; P0704S) or a mixture of O-Glycosidase (NEB ...
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bioRxiv - Biophysics 2020Quote: ... as with commercially available human H1 (hH1) (M2501S; NEB), sharing 96.5% identity with mH1 (Fig ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and human casein kinase II (New England BioLabs, #P6010).
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Recombinant human Histone H2A (New England BioLabs, Catalog # M2502S) or H4 (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 8.0) were incubated with pre-washed pan anti-Kbhb beads (PTM Biolabs Inc., Chicago, IL) at 4 °C overnight with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of human recombinant histone H4 (BioLabs, Cat# M2504S) was incubated with 50 μM n-butyryl- or isobutyryl-CoA and 0.2 μM of HAT1 at 30 °C for 1 hour ...
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bioRxiv - Biophysics 2020Quote: Recombinant wild-type human histone H1.0 (New England Biolabs, cat. # M2501S) was used for experiments with fluorescently-labeled nucleosomes ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human CHIP was expressed in BL21(DE3) (New England Biolabs) E ...
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bioRxiv - Cell Biology 2024Quote: ... coli-derived recombinant human histone H1 (NEB, Ipswich, MA, USA #M2501), H2A (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Afterwards 2 µg of recombinant human Histone H3.1 (New England BioLabs, M2503), 100 µM S-(5′-adenosyl)-L-methionine chloride dihydrochloride (SAM ...
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bioRxiv - Genomics 2019Quote: ... Human genomic DNA was fragmented by a dsDNA fragmentase (New England Biolabs) followed by end preparation and adapter ligation according to the NEBNext Ultra II DNA Library Prep Method for Illumina (New England Biolabs).
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...