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Citations for New England Biolabs :
1 - 50 of 9031 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis of the binding sites for the predicted Caudal and DEAF-1 transcription factors was performed sequentially with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) taking as a template the pGreenRabbit vector containing the FBti0019386 sequence following manufacturer’s instructions (25) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription was performed with either HiScribe T7 ARCA mRNA kit or the HiScribe In Vitro Transcription Kit (NEB) according to the manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2024Quote: ... This fragment was cloned into a lentiviral expression plasmid we have previously described (31) encoding the Notch1 core linked to the tTA transcription factor using NEBuilder HiFi DNA Assembly (New England Biolabs). The resultant plasmid encoded constitutive expression of CII-synNotch receptor from an EF1α promoter and production of the puromycin N-acetyl-transferase transgene via the constitutive PGK promoter for antibiotic selection ...
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bioRxiv - Neuroscience 2020Quote: ... and a reverse transcription kit (NEB, Ipswich, MA) according to the manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... In-vitro-transcription was carried out using the Hi-Scribe RNA transcription kit (New England BioLabs). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription was performed using LunaScript RT SuperMix Kit (NEB). Oligonucleotides used for qRT-PCR are listed in Supp ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions using the Hiscribe IVT kit (New England Biolabs), were assembled to a final volume of 20 μl ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by T7 in vitro transcription using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs, Ipswich, MA) and purification with Trizol (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... Transcription and fluorescent labelling of RNA in vitro transcription reactions were carried out using a T7 RNA transcription kit (HiScribe T7 High Yield; New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: In vitro transcription reactions were carried out using a T7 RNA transcription kit (HiScribe T7 High Yield; New England Biolabs) with the following modifications ...
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bioRxiv - Genomics 2023Quote: ... A transcription reaction was then carried out on the purified and quantified dsDNA using the T7 HiScribe transcription kit (NEB). The T7 RNA Polymerase recognizes the T7 promoter region that seeds transcription of the adjacent 20-mer target sequence thus generating the targeting sgRNA for Cas9-mediated nicking of genomic DNA templates ...
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bioRxiv - Cell Biology 2021Quote: ... SgRNA are produced by T7 Hiscribe transcription kit (New England Biolabs) and purified by EZNA microelute RNA clean up kit (Omega biotek) ...
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bioRxiv - Immunology 2023Quote: sgRNAs were produced using the transcription T7 high yield kit (NEB) and purified with the EZNA micro-elute RNA clean up kit (Omega Bio-tek) ...
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bioRxiv - Cancer Biology 2024Quote: ... The transcription products were purified with Monarch RNA Cleanup Kit (NEB) followed by polyadenylation using Poly(U ...
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bioRxiv - Molecular Biology 2019Quote: The purified PCR products were used as templates for in vitro transcription of antisense and sense ssRNA by using the HiScribe™ T7 In Vitro Transcription Kit (NEB #E2030S ...
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bioRxiv - Biophysics 2022Quote: ... 1 µL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Neuroscience 2022Quote: In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB). The RNA transcripts were extracted using Trizol (Thermo ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was achieved using the M-MuLV Reverse Transcription kit (NEB), according to manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2023Quote: ... WMISH probes were generated via a SP6 or T7 transcription kit (NEB) and prospectively labelled with either Fluorescein or DIG-UTP ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA was synthesized using Proto Script II reverse transcription kit (NEB) and real-time PCR reactions were performed using Quant Studio 3 (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... Further amplification was achieved by in-vitro transcription that was performed overnight using a high-yield in vitro transcription kit (NEB, cat. no. E2050S). Reverse transcription was then performed on the RNA template using Maxima H-Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
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bioRxiv - Plant Biology 2021Quote: ... Factor Xa (P8010S, New England BioLabs) or TEV protease (P8112S ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed using ProtoScript First Strand cDNA Kit (New England Biolabs) according to the manufacturer’s instructions for the synthesis of first strand cDNA ...
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bioRxiv - Systems Biology 2019Quote: ... then in vitro transcription further amplified the oligos (NEB Quick High Yield Kit), t7 reactions were purified with desalting columns ...
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bioRxiv - Neuroscience 2020Quote: ... and carried out in vitro transcription using the HiScribe T7 Kit (NEB, E2040S) by incubating at 37°C for 8 hours ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcription was carried out using the HiScribeT7 High Yield kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... followed by reverse transcription with ProtoScript II First Strand cDNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... gRNA transcription was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol and gRNAs were purified via Phenol:Chloroform:Isoamyl alcohol (25:24:1 ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB) and purified with RNEasy Kit (Qiagen ...
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bioRxiv - Genetics 2019Quote: ... and reverse transcription was performed using LunaScript RT Supermix Kit (New England Biolabs). Oligonucleotides used for qRT-PCR are listed in Supplementary Information Table S3 ...
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bioRxiv - Molecular Biology 2022Quote: ... reverse transcription was performed using ProtoScript II First Strand cDNA Synthesis Kit (NEB) using d(T)23VN primer with DNase I (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... gRNA transcription was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol and gRNAs were purified via Phenol:Chloroform:Isoamyl alcohol (25:24:1 ...
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bioRxiv - Plant Biology 2022Quote: ... In vitro transcription reactions were performed using a Standard RNA Synthesis Kit (NEB). 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription PCR was then performed using LunaScript RT Master Mix Kit (NEB) using a gene specific primer ...
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bioRxiv - Molecular Biology 2023Quote: ... dsRNA was generated by in vitro transcription using the HiScribe T7 kit (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcription was performed using a T7 Quick HiScribe kit (NEB, E2050S) according to manufacturer’s instructions with overnight incubation at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions were purified using the Monarch RNA cleanup kit (New England Biolabs). mRNA concentration was quantified by nanodrop spectrophotometer ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...