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Citations for New England Biolabs :
1 - 50 of 1047 citations for Human Glutaminyl tRNA Synthetase QARS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... coli tRNA (New England Biolabs), 15% formamide (Sigma) ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... coli tRNA (New England Biolabs), 15% formamide (Sigma) ...
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bioRxiv - Microbiology 2022Quote: tRNA was dephosphorylated using QuickCIP (New England BioLabs) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: tRNA was dephosphorylated using QuickCIP (New England BioLabs) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Pre-tRNA was resuspended in nuclease-free water (New England Biolabs) at 100 μM stock concentration ...
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bioRxiv - Biochemistry 2020Quote: ... pre-tRNA stock was diluted 1 in 2 into RNA loading buffer (New England Biolabs) and separated on a 10% acrylamide urea-TBE denaturing gel ...
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bioRxiv - Microbiology 2022Quote: ... Then the tRNA were digested with 10 U Nuclease P1 (New England Biolabs, MA, USA) at 37°C overnight followed by dephosphorylation using 10 U bacteria alkaline phosphatase (Thermofisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The tRNA 2’-3’ cyclic phosphate was removed by treatment with T4 PNK (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... The ncAA naphthalene was ligated to the tRNA with T4 DNA ligase (New England Biolabs, MA, USA). The aminoacylated tRNA was purified with phenol-chloroform extraction and ethanol purification ...
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bioRxiv - Molecular Biology 2021Quote: ... yeast tRNA and poly dIdC was substituted for 20 units of murine RNase inhibitor (New England Biolabs), 12.5 mM DTT ...
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bioRxiv - Molecular Biology 2022Quote: ... and tRNA-Gly-CCC (Table S1) were radioactively labeled with gamma-32P-ATP using T4 PNK (NEB). Pre-hybridization and hybridization were performed in OligoHyb Buffer (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... Dephosphorylation of the 5’-triphosphate pre-tRNA was done using 5 units QuickCIP (New England Biolabs, cat#M0525S) for 30 minutes at 37°C in 1X rCutSmart Buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed with 1xPBS and blocked for 30 minutes at 37°C in blocking buffer (1xPBS, 0.05% BSA [Invitrogen, Am2616], 50μg/mL yeast tRNA [Sigma-Aldrich, R8759], 10mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...
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bioRxiv - Cell Biology 2022Quote: ... The mutated Rop1 expression plasmid and the amber suppressor tRNA plasmid were co-transformed into BL21 (DE3) cells (NEB), as described2 ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 37 °C in Hybridization Buffer (10% Formamide, 10% 20x SSC, 400 µg/ml E. coli tRNA (New England Biolabs), 5% dextran sulfate ...
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bioRxiv - Synthetic Biology 2019Quote: ... An in vitro RED20 prototype was prepared by supplementing PURExpress in vitro expression system lacking tRNAs (PURE ΔtRNA, NEB# E6840S). PURE ΔtRNA supplemented with supplied control tRNAs was used as a Standard Code ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 pmol of unligated tRNA sample were prepared in an equivalent buffer to the first ligation reaction (1X RNA Ligase 2 buffer (NEB), 5% PEG 8000 ...
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bioRxiv - Plant Biology 2020Quote: ... the chain of multiplex tRNA-gRNA with three NsWOX9-spacers was inserted into an optimized vector with AtU6-tRNA-gRNAs-AtUbi10-Cas9-pRGEB31-bar backbone by digestion and ligation using Fok I (NEB) and BsaI enzymes (Xie ...
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bioRxiv - Molecular Biology 2019Quote: ... tRNA containing plasmids were linearized using restriction digestion and tRNAs were in vitro transcribed overnight using the HiScribe T7 transcription kit (NEB) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616; 1 mg/ml E.coli tRNA Sigma R1753; 2 mM Vanadyl-ribonucleoside complex NEB S1402S; 2XSSC) containing diluted probes and incubated over night at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fragments of 10 - 40 nt for partially hydrolyzed and re-phosphorylated tRNAs were converted to small RNA sequencing libraries using NEBNext multiplex small RNA library prep set (NEB) for Illumina kit ...
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bioRxiv - Biochemistry 2020Quote: ... Mature tRNA sequences were obtained by deleting the intron sequence using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). All constructs in this study were verified by Sanger sequencing.
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bioRxiv - Synthetic Biology 2022Quote: ... The genomic tRNA operon of REP12 was PCR amplified using the Q5 Hot Start Master Mix (New England Biolabs, USA) from extracted phage gDNA and purified using the GeneJet PCR purification kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Radioactively labelled tRNAs carrying a 2′,3′ cyclic phosphate at the 3′ end was dephosphorylated using T4 polynucleotide kinase (NEB) in 100 mM Tris-HCl pH 6.5 ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 ng of the amplified tRNA operon was assembled into the linearized pRK2-cat backbone using the HiFi DNA Assembly Master Mix (New England Biolabs, USA). After incubation for 60 mins at 50 °C ...
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bioRxiv - Genomics 2023Quote: ... Included in the gel as controls were an RT reaction conducted in the absence of tRNA and a Small Range RNA Ladder (NEB N0364S). The gel was stained with SYBR gold and visualized using a ChemiDoc imager (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... to remove the 3’-phosphate group from the uncharged tRNA followed by ligation to 5’-adenylated uniquely barcoded adapters using RNA ligase 2 truncated KQ (New England BioLabs, Cat. # M0351L). The resulting tRNAs were then ligated to a 5’-adaptor ...
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bioRxiv - Developmental Biology 2023Quote: ... and then hybridized overnight at 37°C in hybridization buffer (2xSSC, 10% dextran sulfate [Sigma-Aldrich, D8906], 1mg/mL yeast tRNA [Sigma-Aldrich, R8759], 2mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Synthetic Biology 2022Quote: In vitro transcription/translation by codon skipping of the short FLAG tag-containing peptides X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag) where X = 7, 13, 14, or 15 was carried out using the PURExpress® Δ (aa, tRNA) Kit (New England Biolabs, E6840S) based on a previous protocol with slight modifications 16 ...
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bioRxiv - Molecular Biology 2022Quote: The coding sequence of the BTB domain of selected ZBTB family proteins was amplified from cDNA derived from the human HCT116 cell line using Q5 High-Fidelity DNA Polymerase (NEB). Specifically for the Patz1 construct ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant human FICD proteins (aa 104-445) were expressed as His6-Smt3 fusion constructs in T7 Express lysY/Iq (NEB) E ...
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bioRxiv - Biophysics 2019Quote: ... the imaging chamber was incubated in blocking buffer (10 mM Tris HCl pH 7.5, 50 mM NaCl, 1 mg/mL BSA [NEB], 1 mg/mL tRNA [Ambion]) for 1 h ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Microbiology 2019Quote: ... falciparum and human histones (New England Biolabs) (6µg) ...
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bioRxiv - Microbiology 2021Quote: ... 40 U RNase Inhibitor Human Placenta (NEB), 50 pmol Oligo d(T)23 (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... Human CK2 was obtained from NEB (P6010S). IE2-NTD was phosphorylated in buffer provided by the manufacturer which contains 50mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... protein was treated overnight with Lambda Protein Phosphatase (NEB #P0753S) at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... Cas9 protein (NEB) was mixed with all six sgRNAs and injected into embryos at the 1-cell stage ...
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bioRxiv - Plant Biology 2024Quote: ... SpCas9 protein (NEB), 1 x cut smart buffer (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... pFastBac dual vector containing the sequence for the N-terminally 6xHis-tagged human Naa15 and truncated human Naa10 (residues 1-160) sequences was digested using KpnI-HF (NEB) and XmaI (NEB ...
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bioRxiv - Biochemistry 2023Quote: Human HA-DHFR was subcloned from human cDNA to pcDNA4/TO by PCR using Q5 High Fidelity 2X mastermix (NEB). Human FPGS-FLAG and GGH-FLAG were subcloned from cDNA clones MHS6278-202755815 (Horizon Discovery ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... MARCH2 chimeras containing the TM domains from either human MARCH4 or human transferrin receptor (TR) were generated using the NEBuilder HiFi DNA assembly kit (New England Biolabs) and the primers listed in Supplemental Table S2 ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were then mixed with Blue Protein Loading Dye (NEB) boiled for 10 minutes and separated on 10% SDS-PAGE gel for 50 minutes at 140 V ...