Labshake search
Citations for New England Biolabs :
1 - 50 of 10000+ citations for Human Eukaryotic Translation Termination Factor 1 ETF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: In vitro protein translation was carried out (in absence of the release factors) using the PURExpress ΔRF123 kit (New England Biolabs). This guaranteed the stabilization of the ternary complexes mRNA-affitin-Ribosome ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
-
bioRxiv - Genomics 2020Quote: ... using a Eukaryotic directional mRNA library (NEB). cDNA preliminary concentration was quantitated on a Qubit (Life Technologies) ...
-
bioRxiv - Biochemistry 2023Quote: A luminescence based translation inhibition assay was performed using an in-vitro translation PURExpress® Δ Ribosome Kit from (NEB) The constituents of the kit were incubated with 25 ng pMSR DNA template ...
-
bioRxiv - Biochemistry 2019Quote: In vitro translation (IVT) reactions used the PURExpress Δribosome kit (NEB) and a nanoluciferase reporter plasmid (Promega) ...
-
bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
-
bioRxiv - Biophysics 2022Quote: ... 1 µL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
-
bioRxiv - Biophysics 2023Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
-
bioRxiv - Molecular Biology 2022Quote: In vitro translation was carried out using the deltaRF123 Kit (New England Biolabs). 200 ng of mRNA was added per μL of reaction ...
-
bioRxiv - Biophysics 2022Quote: ... an in vitro translation mixture was assembled using a ∆Ribosome PURExpress kit (NEB). The mixture was assembled with the following ...
-
bioRxiv - Synthetic Biology 2024Quote: ... an in vitro translation mixture was assembled using the ΔRibosome PURExpress kit (NEB): 3.2 μL solution A (NEB) ...
-
bioRxiv - Microbiology 2019Quote: In vitro transcribed 3X-FLAG tagged manY transcripts – wild-type or Δenh (1 pmol) were translated in vitro using the PURExpress translation kit (NEB). Translation reactions were stopped by adding Laemmli sample buffer (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... In vitro translation was carried out in 10 µL using the PURExpress kit (NEB) according to the commercial protocol in the presence of a mix of 32P-radiolabeled cspA mRNA (200000 cpm/µL ...
-
bioRxiv - Molecular Biology 2023Quote: Coupled transcription-translation assays were carried out with the PURExpress kit (New England BioLabs) as described in (Lukasiewicz and Contreras ...
-
bioRxiv - Molecular Biology 2019Quote: ... in vitro translation was performed using the PURExpress In vitro Protein Synthesis kit (NEB 6800) and the non-stop ribosome complex was purified as previously described in ref.38 ...
-
bioRxiv - Biochemistry 2022Quote: In vitro coupled transcription-translation was performed at 37 °C using the PURExpress ΔIleRS Kit (NEB) following manufacturer’s protocol supplemented with [35S]-methionine (EasyTag ...
-
bioRxiv - Microbiology 2022Quote: ... In vitro translation reactions were performed using PURExpress ΔRF123 kit and PURExpress ΔRibosome (New England Biolabs) according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2021Quote: The PURExpress ΔRF123 cell-free translation system (NEB) was used with the following modifications ...
-
bioRxiv - Molecular Biology 2023Quote: In vitro translation was carried out with a custom PURExpress ΔRF-123 ΔRibosome kit (New England Biolabs), using ∼5 pmol of 5’-biotinylated and 3’-polyadenylated mRNA as a template ...
-
bioRxiv - Molecular Biology 2022Quote: ... we used the PURExpress translation system (New England Biolabs) reconstituted from purified components ...
-
bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
-
bioRxiv - Genomics 2019Quote: ... nick translation was performed with regular dNTPs (New England Biolabs) and biotin-11-dUTP (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2019Quote: ... we used in vitro PURExpress translation system (New England Biolabs) reconstituted from purified components and supplied with either the WT 70S ribosomes from E ...
-
bioRxiv - Microbiology 2022Quote: ... ShdA III and ShdA IV homologues were synthesised in vitro using the PURExpress cell-free transcription/translation kit (NEB), from PCR products ...
-
bioRxiv - Microbiology 2024Quote: ... 25 °C) using a custom methionine(-) Flexible In vitro Translation system composed by PURExpressTM (ΔRF123) Kit (New England Biolabs) solution B ...
-
bioRxiv - Biochemistry 2024Quote: ... coli constitutive ribosomal promoter J23119 with a poly-T region directly downstream of the sgRNA sequence for transcription termination using KLD mutagenesis (New England Biolabs). pBR322 and pET28 expression plasmids were co-transformed into E ...
-
bioRxiv - Biochemistry 2024Quote: In vitro translation was monitored by the production of luciferase signal in a PURExpress in vitro protein synthesis kit (NEB), using firefly luciferase mRNA as an input ...
-
bioRxiv - Biochemistry 2020Quote: ... The PURExpress in vitro transcription-translation system was purchased from NEB. GeneJet PCR clean-up kit was purchased from Thermo-Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis of the binding sites for the predicted Caudal and DEAF-1 transcription factors was performed sequentially with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) taking as a template the pGreenRabbit vector containing the FBti0019386 sequence following manufacturer’s instructions (25) ...
-
bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
-
bioRxiv - Biochemistry 2020Quote: In vitro trans-translation assays were performed using the PURExpress In Vitro Protein Synthesis and Δ Ribosome kits (New England Biolabs). For trans-translation assays ...
-
bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
-
bioRxiv - Genomics 2023Quote: ... Probes were labeled with 32P by nick translation (New England Biolabs, Inc.) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
-
bioRxiv - Cell Biology 2023Quote: The 2NSP23 nanobody mRNA was prepared by in vitro translation of a DNA molecule of using the HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (New England BioLabs). The obtained mRNA has the following sequence and includes a 5’-CAP and a poly-A tail ...
-
bioRxiv - Microbiology 2024Quote: ... in vitro translation of the short-model mRNA was performed using a PURExpress® In VitroProtein Synthesis Kit (New England Biolabs, US). The reaction mixture (volume ...
-
bioRxiv - Biophysics 2023Quote: ... An in vitro translation reaction was then assembled using the PURExpress system (NEB) with the following ...
-
bioRxiv - Biochemistry 2024Quote: ... For the in-vitro transcription and translation reactions in PURExpress (New England Biolabs), nuclease PCR products were added at 5 nM final concentration and expressed at 37°C for 2 hours ...
-
bioRxiv - Bioengineering 2022Quote: ... Then CaCl2 was added to 2mM and 1 µL (about 1 µg) of Factor Xa (NEB) was added per 50 µg of protein and the samples were left at RT for 20-24h ...
-
bioRxiv - Neuroscience 2021Quote: ... Enrichment of mRNA from eukaryotic organisms was performed using oligo(dT) beads from NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat.No. E7490L, NEB, USA). Subsequently ...
-
bioRxiv - Microbiology 2023Quote: ... and eukaryotic mRNA was removed by binding and discarding the eukaryotic mRNA polyA region to oligo d(T)25 magnetic beads (England Biolabs). Finally ...
-
bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
-
bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
-
bioRxiv - Plant Biology 2021Quote: ... Factor Xa (P8010S, New England BioLabs) or TEV protease (P8112S ...
-
bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates; New England Biolabs, P0702S). All samples were solubilised for 12 h at 37°C and then sonicated prior to resolution by SDS-PAGE (10% or 16% PAGE ...
-
bioRxiv - Biophysics 2022Quote: An in vitro translation reaction was assembled with the following: 6.4 µL solution A (NEB), 2 µL factor mix (NEB) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...