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Citations for New England Biolabs :
1 - 50 of 9760 citations for Human Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... SFP synthase (NEB), and Dy550-CoA (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... SFP synthase (New England Biolabs) and purified ybbR-Luciferase-ybbR were incubated over night at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... using a 5-fold excess of fluorophore to protein and ∼1 μM Sfp synthase (NEB) for 16 hours at 6 °C in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... SFP synthase was purchased from NEB. Methyl beta cyclodextrin was purchased from CycloLab ...
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bioRxiv - Neuroscience 2022Quote: ... and Sfp synthase (New England Biolabs) in Tag-Lite Buffer for 1 hr at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µM ACP synthase (New England Biolabs), and 10 mM MgCl2 for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the codon optimized RBD was PCR amplified and cloned into the bicistronic pDZ rescue plasmid system for A/Puerto Rico/8/1934 using the NEBuider HiFi DNA assembly kit (NEB). Specifically ...
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2020Quote: SNAP-tag ligands (NEB): SNAP-Cell TMR-star ...
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bioRxiv - Neuroscience 2022Quote: ... SNAP-ligand TMR-Star (NEB) or SNAP-surface-Alexa488 (NEB ...
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bioRxiv - Biophysics 2022Quote: ... The human μOR and V2R were amplified with a SNAP tag at their N-terminal (NEB) and subcloned in the pcDNA4/TO plasmid (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... were expressed as fusion proteins with an N-terminal maltose binding protein (MBP)-tag using the pMALTMc5x-vector (New England Biolabs). Cloning was mediated by the addition of the restriction sites XmnI/PstI to the ends of gene fragments PCR-amplified from P ...
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bioRxiv - Synthetic Biology 2019Quote: ... terminal transferase (NEB, M0315L) in 1X terminal transferase reaction buffer (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... and terminal transferase (NEB). Purified extended oligonucleotides were click-labelled with Cyanine5.5 or Cyanine7.5-alkyne (Lumiprobe) ...
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bioRxiv - Molecular Biology 2021Quote: ... tropicalis pseudouridine synthase Pus7 was amplified from total RNA using the OneTaq One-step RT-PCR kit (New England Biolabs). XmaI and XhoI restriction sites were added with oligonucleotides and the resulting fragment was cloned into the p415Gal1 vector.
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bioRxiv - Biophysics 2023Quote: ... We then sequentially labeled Nup96 proteins with SNAP ligand conjugated Alexa Fluor 647 (BG-AF647, S9136S, New England Biolabs) and mitochondria with primary anti-Tomm20 antibodies (rabbit polyclonal ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled with 100 nM Halo-TMR ligand and 100 nM SNAP-Cell 647-SiR ligand (New England Biolabs) for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 U Terminal Transferase (NEB) and ddH2O to a volume of 50 µl ...
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bioRxiv - Biophysics 2021Quote: The assembly of GRB2 SH3 and PSD95 PDZ mutant libraries in both assay plasmids were done overnight by temperature-cycle ligation using T4 ligase (New England Biolabs) according to the manufacturer's protocol ...
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bioRxiv - Microbiology 2022Quote: ... NNK-mutagenized HA amplicons were then cloned into pDZ vector by T4 ligation (T4 DNA Ligase, New England BioLabs Inc.). The ligation product was then used to transform DH10B competent cells (MegaX DH10B T1 ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal deletions were made using the HiFi DNA cloning kit from NEB to excise portions of the N-terminus of the gene ...
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bioRxiv - Biophysics 2022Quote: ... and 0.12 µM SiR-SNAP-Tag ligand (NEB, S9102S) for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Terminal deoxynucleotidyl transferase (New England Biolabs) was then used to conjugate ATTO 633-ddUTP to the 3’ ends of oligonucleotides that had been purchased from IDT ...
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bioRxiv - Genetics 2020Quote: ... 4 Units of terminal transferase (NEB) were added together with dCTP in a final concentration of 0.1 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... and terminal transferase (New England Biolabs). For fluorescent substrates ...
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bioRxiv - Bioengineering 2024Quote: ... using terminal deoxynucleotidyl transferase (TdT) (NEB). This reaction was carried out in 1× TdT buffer ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... SNAP-tag® ligand Blue 430 (New England Biolabs, S9109S) was applied for 30 min at a final concentration of 2 µM ...
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bioRxiv - Cell Biology 2021Quote: ... Amino-terminal mutations were generated by following the Q5® Site-Directed Mutagenesis Kit (NEB #E0554) protocol ...
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bioRxiv - Biochemistry 2021Quote: pMAL-c4E vectors carrying in-frame fusions of the EFR cytoplasmic domain with the N-terminal maltose-binding protein (MBP) tag were transformed into Rosetta 2 cells (NEB) for recombinant protein expression ...
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bioRxiv - Biophysics 2019Quote: ... was generated as a fusion construct with N-terminal maltose binding protein (MBP) as we described previously (45) using the pMAL-c2x (New England Biolabs) variant pMALX (46) ...
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bioRxiv - Immunology 2020Quote: For recombinant CARD proteins, NLRP1 constructs (985-1473, 1213-1473, 1374-1466, 1374–1473) with or without C-terminal SNAP tag (NEB), CARD8–CARD (451-537 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fusions to the miniGs-399 protein with C-terminal truncations to RXFP1 were constructed using PCR followed by NEBuilder HiFi DNA Assembly (New England Biolabs). Truncations removed 10 ...
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bioRxiv - Molecular Biology 2023Quote: ... with GFP protein fused at C- terminal and NcoI restriction enzyme sites on both ends using Gibson Assembly (NEB, USA). Empty vector pCAMBIA 1302 was used as a positive control ...
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bioRxiv - Plant Biology 2024Quote: ... in an N-terminal fusion (MIROs are tail anchored proteins) with mCherry that was generated by Gibson assembly (New England Biolabs, Ipswich ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 unit/μl Terminal Transferase (NEB). The DNA was incubated at 37 °C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... 0.2 U/μl Terminal Transferase (NEB, M0315L)) and incubated at 37 °C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... was used to generate the CAR.PDZ with a PDZbm attached to the C-terminus of CD3ζ domain and site directed mutagenesis Q5(New England Biolabs, Ipswich, MA, USA) was used to do a point mutation to exchange the final Valine to an Alanine to create the CAR.PDZmut construct.
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB, S9234S]) at final concentrations of 1 μM in 0.3% PBT ...
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of Adgrd1 N-terminal mutations was carried out using Q5 site directed mutagenesis kit (NE Biolabs) using manufacturers protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... A C-terminal HA tag was added using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For microinjection ...
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bioRxiv - Neuroscience 2022Quote: ... was cloned into pDEST17 vector containing an N-terminal His10-Smt3-tag and a C-terminal SNAP-tag (New England BioLabs). The exact same purification scheme for DDX5(1-535)-SNAP was used to purify DDX5(1-483)-SNAP and the protein was flash frozen in medium salt storage buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: The full-length African killifish DDX5 was cloned into the pDEST17 vector containing an N-terminal His10-tag and a C-terminal SNAP-tag (New England BioLabs). E ...
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bioRxiv - Neuroscience 2022Quote: The full-length African killifish DDX5 was cloned into the pDEST17 vector containing an N-terminal His10-Smt3-tag and a C-terminal SNAP-tag (New England BioLabs). E ...
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bioRxiv - Neuroscience 2022Quote: ... was cloned into the pDEST17 vector containing an N-terminal His10-Smt3-tag and a C-terminal SNAP-tag (New England BioLabs). The same purification scheme was used to purify DDX5(1-535)-SNAP than DDX5-SNAP ...
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bioRxiv - Synthetic Biology 2019Quote: ... in 1X terminal transferase reaction buffer (NEB, M0315L) and 0.25 mM CoCl2 (NEB ...
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bioRxiv - Genetics 2021Quote: ... 4 units Terminal Transferase (New England Biolabs™) and were carried out at 37°C for 30 minutes followed by heat inactivation at 75°C for 10 minutes ...
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bioRxiv - Biophysics 2021Quote: ... using a terminal transferase (New England BioLabs, M0315S) as described previously.[21] Briefly ...