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Citations for New England Biolabs :
1 - 50 of 221 citations for Hrms Rapid Pcb Screening Calibration Solution Cs0.05 Unlabeled 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... or additional unlabeled ATP (NEB) were added to each reaction as indicated ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μL of Rapid PNGaseF (NEB #P0710S) was added and incubated at 50°C for 1 hour to cleave N-glycans from proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... using 55 fmol of purified cassette after BbsI-HF (for photoautotrophy screening with p1 plasmids) or BsaI-HF (for antibiotic screening with pM plasmids) digestion (New England Biolabs) of the corresponding plasmid ...
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bioRxiv - Biochemistry 2019Quote: ... unlabeled AdoMet was left out and 6 μg of MBP2* protein (NEB) was added to each reaction to increase molecular crowding.
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bioRxiv - Molecular Biology 2023Quote: Unlabeled purified RNA targets were dephosphorylated using 1 U antarctic phosphatase (NEB) incubated with 1X of provided buffer during 20 min at 37°C and then 5 min at 65°C to inactivate the enzyme ...
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bioRxiv - Biophysics 2023Quote: ... the digoxigenin-labeled and unlabeled amplicons were ligated with T7 ligase (New England BioLabs) in a specific ratio of 1:2 ...
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bioRxiv - Neuroscience 2023Quote: ... Screening of potential mutants was performed using T7 endonuclease (NEB) treatment of PCR products amplifying the second opn4xa exon (Fw ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were purified with Exo-CIP Rapid PCR Cleanup Kit (NEB) following supplier’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... or the Exo-Cip Rapid PCR Cleanup Kit (New England Biolabs). Gene editing efficiency was analyzed using TIDE analysis software (Brinkman et al ...
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bioRxiv - Bioengineering 2023Quote: ... Vectors for in vitro screening were constructed via Gibson assembly (NEB, E2611S). KKH-saCAs9 AAV vectors are based on PX601 (Addgene 61591)69 ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were cooled to 4°C for 30 seconds and quenched with 1.2 mM unlabeled SAM (NEB) and blotted onto Hybond-XL membrane ...
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bioRxiv - Genomics 2022Quote: ... the 1:99 target:background sample was digested with FspEI (New England Biolabs) according to the manufacturers protocol (incubation at 37°C for 90 minute ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of unlabeled crRNA was mixed with equal volume of 2 × RNA loading dye (New England Biolabs) and fractionated in the 12% denaturing polyacrylamide gel ...
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bioRxiv - Genomics 2021Quote: ... The screening vector was linearized with AgeI-HF and SalI-HF restriction enzymes (NEB) and purified through electrophoresis and gel extraction ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was PCR-amplified for 25 cycles with 5’-Cy3-labelled reverse primers (IDT) and unlabeled forward primers using either Taq polymerase or Phusion high-fidelity polymerase (NEB). PCR products were separated on 40cm tall 6% polyacrylamide denaturing gels and then visualized using a Molecular Dynamics Typhoon Scanner ...
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bioRxiv - Developmental Biology 2021Quote: DNA probes were generated by annealing 5’ IRDye®700 labeled forward oligonucleotides with unlabeled reverse oligonucleotides (Integrated DNA Technologies) to a final concentration of 5 μM in PNK buffer (New England Biolabs). One hundred femtomoles of labeled IRDye®700 probes were used in a 20-μl binding reaction containing 10 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... and the radiolabeled 3’ fragment was ligated to an unlabeled 5’ fragment by splinted ligation with T4 DNA ligase (NEB) at 30°C for 2 h ...
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bioRxiv - Microbiology 2020Quote: A template-switching 5’ rapid amplification of cDNA ends (5’-RACE) kit (New England BioLabs) was used to map the transcription start site of rflP ...
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bioRxiv - Bioengineering 2019Quote: ... Unlabeled tracrRNAs were synthesized by IDT or through in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB) using DNA templates containing a T7 promoter binding sequence and full length tracrRNAs ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid length DNA binding experiments were performed with unlabeled circular or linearized M13mp18 single (New England Biolabs, M13mp18 single-stranded DNA) or double-stranded DNA (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... screening and validation of DNA sequences were performed using Taq DNA Polymerase (New England Biolabs, USA). T4 DNA ligase and restriction enzymes were purchased from New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: Q5 DNA Polymerase was used for DNA amplification and colony PCR screening (New England Biolabs, Germany). Gibson Assembly and site-directed mutagenesis was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Successful amplicons were purified using the Exo-CIP Rapid PCR Cleanup Kit (New England BioLabs®) and sent for bi-directional Sanger sequencing with Eurofins Genomics.
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 µL of Rapid PNGase F (New England Biolabs, Fig. 2d and Extended Data Fig. 2a) were added with 1.5 µL of 10× PNGase F buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μL of plasma was lyophilized and resuspended in 20 μL 1X Rapid PNGaseF buffer (NEB #P0710S) and incubated for 15 minutes at 70°C to denature proteins ...
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bioRxiv - Bioengineering 2021Quote: ... All experiments for the calibration curve with Influenza and SARS-CoV-2 were performed with the Colorimetric LAMP mix from NEB. Real-time colorimetric LAMP (qcLAMP ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration in the growth medium was quantified by measuring fluorescence at 610 nm following excitation at 575 nm in comparison of signals with a calibration curve prepared from mCherry which was cloned into pQE30 (NEB), expressed in E ...
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bioRxiv - Cell Biology 2020Quote: PCR amplification of short fragments for screening purposes (<3 kbp) was conducted using OneTaq® (New England Bolabs (NEB), Ipswich ...
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bioRxiv - Developmental Biology 2022Quote: ... Histone yields from each purification were determined by using a calibration curve of recombinant H3 and H4 (NEB, cat. #M2503S, #M2504S, respectively). Absolute amounts of histone were determined by densitometry on coomassie-stained bands with Fiji61 for Pristionchus data (conducted at the MPI in Tübingen ...
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bioRxiv - Microbiology 2019Quote: ... Cloning and screening PCR reactions were performed using Q5 High-Fidelity DNA and One-Taq DNA polymerases (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2020Quote: ... F0 founders were identified by outcrossing TALEN or CRISPR/Cas9-injected fish with wildtype fish and screening the offspring for mutations at 2 dpf using T7 endonuclease I (NEB) assay (for TALEN-injected fish ...
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bioRxiv - Microbiology 2023Quote: Abortive/PCD systems identified by transposon screening of conditionally lethal AGs were cloned by Gibson assembly using the NEBuilder HiFi DNA assembly master mix (NEB) onto pBAD Myc/His A vectors along with their native promoters wherever possible ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase chain reactions (PCR) were performed with Taq polymerase (Feldan) for screening purposes or Q5 high-fidelity DNA polymerase (New England Biolabs) for cloning and site-directed mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Synthetic Biology 2023Quote: ... A 5 µL aliquot was taken in which primers and dNTPs were then inactivated using Exo-CIP Rapid PCR Cleanup Kit (NEB). From the resulting mixture ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Microbiology 2019Quote: ... Cloning and screening PCR reactions were performed using Q5 High-Fidelity DNA and One-Taq DNA polymerases (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2019Quote: ... Colonies were selected using a gentamycin marker and blue/white colony screening in a background of dH5-alpha cells (New England Biolabs, UK). The resulting construct was transformed into PA14 and the clone used for assays was verified by Sanger sequencing (University of Sheffield ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were constructed using the Swift Rapid Library Prep Kit (Swift Bio #R2096) with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490L) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 2 µl of genomic DNA was used for screening by PCR amplification (Phusion® High-Fidelity PCR Master Mix with HF Buffer, NEB, M0531S) of the targeted genomic region ...
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bioRxiv - Molecular Biology 2024Quote: ... 173 bp TBR-PCR target products were excised and purified using an Exo-CIP Rapid PCR Cleanup Kit (New England Biolabs, Ipswich, USA) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PURExpress® kit containing Solution A and Solution B (NEB) was used to prepare the transcription-translation reaction ...
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bioRxiv - Genomics 2020Quote: ... 50-100 fmol of PCR products were diluted in 9 µl then mixed with 1 µl of Rapid 1D Adapter and 1 µl of Ligase T4 Blunt (New England Biolabs, Ipswich, MA, USA). Pre-sequencing mix was incubated 10 min at 25°C and left on ice until ready to load ...
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bioRxiv - Developmental Biology 2022Quote: ... Products were visualized on 2% agarose gels and successful amplification reactions were prepared for Sanger sequencing with Exo-CIP™ Rapid PCR Cleanup Kit according to the manufacturer’s protocol (New England Biolabs: E1050S, Ipswich, MA). Samples were then sequenced with amplification primers (Eurofins Genomics Services ...
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bioRxiv - Microbiology 2023Quote: ... PCR was conducted using a Rapid Barcoding Kit 96 (ONT, Oxford, UK) with Q5® Hot Start High-Fidelity 2X Master Mix (NEB, Ipswich, MA) according to the manufacturer’s protocol designed for SARS-CoV-2.
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Synthetic Biology 2024Quote: ... 3.2 μL solution A (NEB), 1 μL factor mix (NEB) ...
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bioRxiv - Microbiology 2019Quote: ... The reaction solution was prepared in the following order: 10 μL solution A (NEB #E6800), 7.5 μL solution B (NEB #E6800) ...
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bioRxiv - Microbiology 2019Quote: ... 7.5 μL solution B (NEB #E6800), 2 μL E ...