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Citations for New England Biolabs :
1 - 50 of 498 citations for Histone Lysine N Methyltransferase EZH2 EZH2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... were introduced in full-length ezh2 cDNA by the Q5® Site-Directed Mutagenesis Kit (NEB; E0544S). Resulting plasmids were validated by Sanger sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... and methyltransferase (NEB# M0366S) by following the vendor’s manuscripts.
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Bioengineering 2020Quote: ... and Cap 2’-O-Methyltransferase (NEB), followed by another purification by RNA Clean & Concentrator (Zymo) ...
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bioRxiv - Biophysics 2021Quote: ... and 2’-O-methyltransferase (NEB, M0366), following the one step protocol ...
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bioRxiv - Zoology 2022Quote: ... mRNA cap 2’-O-methyltransferase (NEB) and E ...
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bioRxiv - Genetics 2021Quote: ... EcoRI methyltransferase reaction buffer (New England Biolabs; 50 mM Tris-HCl ...
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bioRxiv - Genomics 2021Quote: ... treated with M.SssI CpG Methyltransferase (NEB #M0226).
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bioRxiv - Molecular Biology 2023Quote: ... SssI CpG methyltransferase enzyme (New England BioLabs) according to manufacturer recommendations ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1x Dam methyltransferase buffer (NEB CN# M0222) and 640 µM AdoMet (NEB CN# B9003) ...
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bioRxiv - Genomics 2022Quote: ... For the GpC methyltransferase treatment, freshly made GpC methyltransferase mix (1x M.GpC buffer, 300 mM sucrose, 64 µM SAM (NEB, #B9003S)) and 200 U M.CviPI (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 µL (12 units) M.SssI methyltransferase (NEB). This solution was pre-warmed to 37°C before addition to prevent interference with dCas9:gRNA binding to the DNA ...
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bioRxiv - Biochemistry 2022Quote: ... the plasmid was with HpaII methyltransferase (NEB, M0214S) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and Vaccinia 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Immunology 2022Quote: ... and Vaccinia 2’ O-methyltransferase (New England Biolabs). The mRNA was purified utilizing oligo-dT affinity chromatography and encapsulated in an LNP through a modified ethanol-drop nanoprecipitation process ...
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bioRxiv - Genomics 2023Quote: ... 200U EcoGII Methyltransferase enzyme (New England BioLabs, M0603S), Nuclease-free Water up to 500μl ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, M0366S). Then ...
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bioRxiv - Genomics 2019Quote: ... DNA was methylated in vitro using M.SssI methyltransferase (NEB) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Placental DNA treated with SssI methyltransferase (New England Biolabs) was used as a positive control for methylated alleles of MGMT ...
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bioRxiv - Genetics 2021Quote: ... and 40 units of EcoRI methyltransferase (New England Biolabs). After incubation for 2.5 h at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... and 20 U CpG methyltransferase M.SssI (New England BioLabs) (20 μL total volume ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, catalog #M0366S). We then added a 3′-poly(A ...
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bioRxiv - Molecular Biology 2019Quote: The construct was in vitro methylated with HpaII methyltransferase (NEB), which recognises the CCGG sequence and modifies the internal cytosine residue ...
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bioRxiv - Plant Biology 2023Quote: ... Histone (NEB).
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bioRxiv - Systems Biology 2019Quote: ... vaccinia mRNA 2’-O-methyltransferase (NEB, 250 U every 2 h) and water ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA treated with a CpG Methyltransferase M.SssI (New England Biolabs) served as positive control ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2′ O-methylated using Vaccinia VP39 (2′ O Methyltransferase) (NEB) in a reaction that also included 1X capping buffer (NEB) ...
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bioRxiv - Genetics 2021Quote: In vitro methylation reactions assaying methyltransferase activity of dam or HpaII (NEB) on DNA were performed in the buffer supplied with the commercial recombinant enzyme (New England Biolabs (NEB ...
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bioRxiv - Genomics 2022Quote: ... Nonspecific adenine methyltransferase EcoGII (New England Biolabs, high concentration stock 25,000U/mL) was added to the reaction and incubated at 37 for 30 min with 300rpm shaking every 2 min ...
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bioRxiv - Biochemistry 2023Quote: ... and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs). The IRES-containing mRNAs were uncapped and polyadenylated.
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bioRxiv - Molecular Biology 2023Quote: Isolated nuclei were subjected to GpC methylation using M.CviPI GpC methyltransferase (NEB), followed by proteinase K and RNaseA treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... and embryos were placed in 2.5 μl GpC methyltransferase (M.CviPI, M0227, NEB) reaction buffer at 37°C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... Plasmid DNA was in vitro methylated using CpG Methyltransferase (M.SssI, New England Biolabs), with the efficiency of methylation assessed by digestion using HpaII and HhaI methylation-sensitive restriction enzymes (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2’O-methylated using Vaccinia VP39 (2’O Methyltransferase) (New England Biolabs), then purified by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Genetics 2019Quote: ... following the manufacturer’s instructions and subsequently methylated with M.SssI methyltransferase (New England Biolabs) overnight at 37°C ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... histone H4 (NEB #2504S), recombinant His-LSF protein ...
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bioRxiv - Biophysics 2020Quote: Histones H1 (M2501S; NEB), H2A/H2B (M2508S ...
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bioRxiv - Genomics 2021Quote: ... the same amplicons were treated in vitro with the recombinant CpG methyltransferase M.SssI (NEB). Briefly ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
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bioRxiv - Genomics 2023Quote: ... Then GpC methyltransferase footprint was performed by incubating the nuclei with M.CviPI (NEB, M0227L), and PBS was added to stop the reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.13 mM S-adenosylmethionine) and 50uL of 4,000U/mL M.CviPI GpC methyltransferase (NEB, M0227L). Each methylation reaction was then incubated for at 37 °C shaking at 1000 rpm for 7.5 minutes (unless specified otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used in the study were: anti-acetylated lysine (PTM Biolabs, 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... to generate Cap 0 and in some cases also the Vaccinia 2’ O methyltransferase (NEB) to generate Cap 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Linear DNA fragments were methylated at their ecoRI sites with ecoRI methyltransferase (New England Biolabs) and transformed into C ...
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bioRxiv - Genomics 2023Quote: ... lambda phage DNA was in vitro CpG methylated using the M.SssI methyltransferase (New England Biolabs, M0226M) with S-adenosylmethionine (SAM ...
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bioRxiv - Genomics 2023Quote: Nuclei were then first treated with M.CviPI (GpC methyltransferase) by adding 200 U of M.CviPI (NEB), SAM at 0.6 mM and sucrose at 300 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Histone H10 was purchased from NEB. In vitro kinase assays were performed as described previously (Harashima & Schnittger ...
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bioRxiv - Microbiology 2021Quote: ... we methylated in vitro the whole pLTR-Fluc construct using the SssI methyltransferase (New England Biolabs, M0226). The LTR fragment was then purified ...
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bioRxiv - Microbiology 2022Quote: ... 23 nt RNA (5’-GAAUCUAUACAUAAAGACCAGGC-3’) was capped with vaccinia capping enzyme and 2’-O-methyltransferase (NEB) and radiolabelled with [α 32P]-GTP ...
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bioRxiv - Biochemistry 2023Quote: ... VCE or FCE::T7RNAP fusion and 5 U/μL vaccinia cap 2′-O-methyltransferase (New England Biolabs). Reactions were carried out at indicated temperatures for 1 h ...