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Citations for New England Biolabs :
1 - 12 of 12 citations for H2 Q10 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously[40] ...
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously (19).
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bioRxiv - Genomics 2019Quote: ... and 0.2 U/µl of RNase H2 (M0288L, NEB) in 1 × NEBuffer 4 (both enzymes in the same reaction) ...
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bioRxiv - Molecular Biology 2021Quote: ... The relaxed plasmid in control samples was generated by treatment of the supercoiled plasmid with RNase H2 (New England Biolabs) for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... of full-length SEC16A were generated by mutating 10 nucleotides in the silencer-select Sec16A siRNA (5’-CGGAGCUUCUGUUACGAGATT-3’, siRNA id: s19236, Thermo-Fischer Scientific) binding site using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions.
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bioRxiv - Plant Biology 2019Quote: ... the corresponding dsRNAs were processed in 18-25 nt siRNAs by ShortCut® RNase III (NEB, Ipswich, MA) for 40 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Long dsRNAs were digested to siRNAs and purified according to the instructions of ShortCut® RNase III (NEB). We then co-transfected COS7 cells with the Ift reporter constructs ...
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bioRxiv - Biochemistry 2019Quote: peYFP-C1-PALB2 was modified to be resistant to PALB2 siRNA by Q5 Site-Directed Mutagenesis Kit (NEB, E0554) using primers JYM3892/3893 (Table S4) ...
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bioRxiv - Cell Biology 2020Quote: ... The open reading frame for a KIF18A wild-type siRNA resistant construct51 and pEM791 vector49 were amplified with primers designed for Gibson Assembly (New England BioLabs). After confirming the correct sequence of the Gibson assembled plasmid ...
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bioRxiv - Biochemistry 2020Quote: ... NC-siRNA sense: 5’-AGGUAGUGUAAUCGCCUUGdTdT-3’.35,36 NEBuffer™ 2 and nucleoside digestion mix were obtained from NEB (Ipswich, MA). Adenosine and N6-methyl adenosine (m6A ...
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bioRxiv - Genomics 2022Quote: ... for 2 hours and then crosslinked with p19 siRNA binding protein (1 μg/ml in depc-PBS; New England Biolabs) or anti-N6-methyladenosine (m6A ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids and siRNAs Bacterial expression constructs for His6-tagged p97 fragments were generated by Q5 Site-Directed mutagenesis (New England Biolabs) of pET15b-His6-p97 plasmid ...