Labshake search
Citations for New England Biolabs :
1 - 50 of 170 citations for Forkhead Box N1 FOXN1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: The three NMDAR fusion protein constructs were subcloned into pCSE2.7-mIgG2a-Fc-XP (N1-Fc and N2B-Fc) or pCSE2.8-mIgG2a-Fc-Xp (N1-N2B-Fc) using NcoI/NotI (New England Biolabs, Frankfurt, Germany) for mammalian production in Expi293F cells ...
-
bioRxiv - Cell Biology 2019Quote: ... the pEGFP-N1 vector was restricted with Nde1 and Not1 enzymes (NEB), and a new multiple cloning site was inserted into the vector instead of sequences corresponding to the CMV promoter and EGFP protein ...
-
bioRxiv - Biochemistry 2022Quote: ... the 6XHis-GB1-Aurora B (59-361 aa) and IN-box (790-844 aa; IN-boxΔC) coding regions were introduced in the pTXB1 plasmid (NEB) by in-fusion cloning using the Nde1 and Sap1 cloning site ...
-
bioRxiv - Cell Biology 2020Quote: ... 1μl of each lysate was used as a PCR template to amplify 600-800 bp fragments around the edited E-box using the Q5 High-Fidelity 2X Master Mix (NEB). The resulting PCR products were purified in 96-well plates using the AmpliClean DNA Cleanup Kit (Nimagen ...
-
bioRxiv - Biophysics 2023Quote: ... The DNA fragment and the pEGFP-N1 backbone were digested using BsrGI-HF (#R3575SVIAL, NEB) and BamHI-HF (#R3136SVIAL ...
-
bioRxiv - Cell Biology 2023Quote: ... Purified PCR products and pAcGFP1-N1 were incubated with XhoI and BamHI (New England Biolabs) under 37 °C for 4 hrs in 1X buffer 3 supplemented with 1% BSA ...
-
bioRxiv - Plant Biology 2022Quote: ... the full region including the HrpL box promoter was PCR-amplified using primers (Supplementary Table S2) and Q5 High-Fidelity DNA Polymerase (NEB, USA). The resulting PCR fragment was gel-purified and was blunt-end-ligated into the Eco53kI (NEB ...
-
bioRxiv - Biophysics 2023Quote: ... The DNA fragment and the SRC-EGFP-pEGFP-N1 backbone were digested using BsrGI-HF (#R3575SVIAL, NEB) and BamHI-HF (#R3136SVIAL ...
-
bioRxiv - Cell Biology 2023Quote: ... The obtained inserts and mCherry-N1 were digested with restriction enzymes (NheI and EcoRI, New England Biolabs) then ligated with T4 DNA ligase (ThermoFisher ...
-
bioRxiv - Plant Biology 2021Quote: ... the mutations were generated in pBridge-PIF3-N1 with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The primers used to generate the m1 to m15 mutants are listed in Supplementary Table 2 ...
-
bioRxiv - Cell Biology 2020Quote: ... into BamHI/SalI sites of the pQCXIP-N1 vector using the T4 DNA ligase (New England BioLabs, #M0202S) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... pLVX mCherry N1 (Clonetech Laboratories Inc., CA) was digested with XhoI and BamHI (New England BioLabs Inc., MA) according to manufacturer instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... This was confirmed in N1 heterozygous AtrxR245C/+ females after digestion of the wild-type allele using FspI (NEB R0135S) and gel purification of the uncut mutant band ...
-
bioRxiv - Neuroscience 2023Quote: ... pSNAP-N1 was generated in-house by Hannes Gonschior by sub-cloning pSNAPf (New England BioLabs Inc., Cat#N9183S) via PCR and the restriction enzymes AgeI and NotI into pmCherry-N1 ...
-
bioRxiv - Cell Biology 2023Quote: ... and N1 mice from these crosses were genotyped as above using a proof-reading polymerase (NEB Q5 DNA Polymerase). Purified amplicons from these PCR reactions were then Sanger sequenced to ensure accuracy of insertion site and genomic sequence ...
-
bioRxiv - Bioengineering 2019Quote: ... the GFP cassette between KpnI/HpaI restriction sites of plasmid GFP-Fibrillarin was replaced with a 730 bp Cerulean cassette PCR amplified from plasmid pCerulean-N1 (New England Biolabs) using primer pair ForCerFib/RevCerFib ...
-
bioRxiv - Biophysics 2022Quote: Saturation mutagenesis of mEosEM at His63 was performed in the pEGFP-N1-mito-mEosEM plasmid with Q5 high fidelity polymerase (New England Biolabs). The amplified fragments containing homologous arms and mutation sites were transformed into the Top10 competent cells (Tsingke ...
-
bioRxiv - Cell Biology 2022Quote: pHALO-C1 and pHALO-N1 were generated by replacing the eGFP in Clontech vectors with HALO using Gibson assembly (E2621L; New England Biolabs). HALO-ARHGEF10 ...
-
bioRxiv - Developmental Biology 2022Quote: ... The resulting PCR fragment and the peGFP-N1-1 vector were sequentially digested with SalI and SacII prior to ligation with Quick ligase (New England Biolabs) to make a pcachd1-eGFP-N1-1 plasmid ...
-
bioRxiv - Cell Biology 2023Quote: pHaloTag-C1 and pHaloTag-N1 were generated by replacing the eGFP in Clontech vectors with HaloTag using Gibson assembly (E2621L; New England Biolabs). HaloTag-ACBD3 ...
-
bioRxiv - Microbiology 2024Quote: ... The CUP1 promoter sequence was amplified from the iBFG-Y2H vectors (primers oHM487/488) and cloned into N2H N1 and N2 vectors digested with SpeI/SacI using NEBuilder assembly (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The obtained pri-miRNA sequences were cloned into the pEGFP-N1 backbone vector by using AgeI and XbaI restriction enzymes and T4 DNA ligase (NEB BioLabs, USA). For shRNA overexpression ...
-
bioRxiv - Cell Biology 2019Quote: ... GST:Importin-β was made by cloning Importin-β cDNA from the pEGFP-N1 vector into pGEX-4T using NcoI and NotI (New England Biolabs). The anillin constructs for mammalian cell expression (GFP-tagged ...
-
bioRxiv - Cell Biology 2020Quote: ... The PCR inserts were assembled with restriction enzyme digested pmCherry-N1 backbone in Gibson Assembly Master Mix (New England Biolabs; E2611S) as per manufacturer recommendations and the assembled plasmids transformed into NEB5a cells (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... The APOE3 ORF was amplified from APOE3-TurboGFP and subcloned into an mEmerald-N1 backbone via Gibson assembly using HiFi DNA Assembly Master mix (New England Biolabs, E2621) to make APOE3-mEm ...
-
bioRxiv - Molecular Biology 2020Quote: ... The obtained pri-miRNA sequences were cloned into the pEGFP-N1 backbone vector by using AgeI and XbaI restriction enzymes and T4 DNA ligase (NEB BioLabs, USA). For shRNA overexpression ...
-
bioRxiv - Biochemistry 2020Quote: ... pGFP2-N3 and pRluc-N1 for POR) after each was digested by the indicated pair of restriction enzymes (New England Biolabs, Inc.; Ipswich, MA). The multiple cloning sites of the vectors are upstream of the GFP or Rluc tag ...
-
bioRxiv - Molecular Biology 2021Quote: ... sequence of iRFP670 amplified from piRFP670-N1 vector by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) and primers encoding NcoI and XmaI digestion sites (Supplementary Table S1) ...
-
bioRxiv - Cancer Biology 2022Quote: ... antibody and anti-Gluc antibody (New England Biolabs, ref. E8023).
-
bioRxiv - Developmental Biology 2023Quote: ... a MBP antibody (Anti-MBP Monoclonal Antibody, HRP conjugated, NEB), a GST antibody (GST Tag Monoclonal Antibody ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein blots were incubated with primary antibodies overnight at 4°C using the above-mentioned antibodies and the Gaussia Rabbit Polyclonal Antibody (E8023S, New England BioLabs), the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568 ...
-
bioRxiv - Plant Biology 2021Quote: ... and anti-MBP antibodies (NEB), respectively.
-
bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibodies (NEB).
-
bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (NEB).
-
bioRxiv - Cancer Biology 2019Quote: ... Bound antibodies were detected by incubation with DyLight 800-conjugated secondary antibody (New England BioLabs). An InnoScan 710-IR scanner (Innopsys ...
-
bioRxiv - Cancer Biology 2024Quote: ... Bound antibodies were detected by incubation with DyLight 800-conjugated secondary antibodies (New England BioLabs), and analysed using an InnoScan 710-IR scanner (Innopsys) ...
-
bioRxiv - Plant Biology 2021Quote: ... Anti-MBP murin monoclonal antibody (Biolabs) was immobilized (around 11000 responsive units (RU) ...
-
bioRxiv - Biochemistry 2023Quote: ... anti-Kcr antibody (PTM Biolabs, PTM502), anti-Ub antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Developmental Biology 2019Quote: ... Bound antibodies were detected by incubation with anti-rabbit DyLight 800-conjugated secondary antibody (New England BioLabs). Slides were analysed using an InnoScan 710-IR scanner (Innopsys) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Bound antibodies were detected by incubation with anti-rabbit DyLight 800-conjugated secondary antibody (New England BioLabs). Slides were analysed using an InnoScan 710-IR scanner (Innopsys) ...
-
bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
-
bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201] ...
-
bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
-
bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs), or mouse monoclonal anti-β-actin antibody (A5316 ...
-
bioRxiv - Biochemistry 2020Quote: ... Anti-butyryllysine antibody (PTM BioLabs, PTM#301) at 1:2000 dilution was incubated with the membrane overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Bound antibodies were detected by incubation with anti-rabbit or anti-mouse DyLight 800-conjugated secondary antibodies (New England BioLabs). Slide images were acquired using an InnoScan 710-IR scanner (Innopsys ...
-
bioRxiv - Biochemistry 2020Quote: ... Primary antibodies used were: New England Biolabs (NEB), Hitchin ...
-
bioRxiv - Molecular Biology 2022Quote: ... and anti-MBP antibody (NEB E8032L; 1:10,000) in 1x TBST supplemented with 5% low-fat milk were incubated either at 4 °C overnight or 1 hr at room temperature ...
-
bioRxiv - Immunology 2022Quote: The antibody expression vectors for each recombinant antibody (VH + VL-L/k) were transfected together with a Transposase vector (Hera BioLabs, USA). Cells were selected with Hygromycin B (H3274 ...
-
bioRxiv - Neuroscience 2021Quote: ... and then incubated for 30 minutes in PBS containing the primary antibodies: rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs, Hangzhou, China), mouse monoclonal anti-tubulin β3 (801201 ...