Labshake search
Citations for New England Biolabs :
1 - 50 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2019Quote: ... Genomic DNA from each fish was digested with PstI-HF (New England Biolabs) and ligated to Illumina P1 adaptors with 6 bp inline barcodes ...
-
bioRxiv - Cell Biology 2021Quote: ... Sonicated salmon sperm DNA was then diluted to 1 mg/ml in Terminal Transferase buffer (NEB) with 0.25 mM CoCl2 ...
-
bioRxiv - Bioengineering 2020Quote: ... Constriction was performed by diluting a library to approximately 8 fM (~5,000 molecules/μl) in salmon sperm DNA (1 ng/μl, New England Biolabs) in low-binding tubes (LoBind ...
-
bioRxiv - Cell Biology 2020Quote: ... F0 founders were identified by outcrossing TALEN or CRISPR/Cas9-injected fish with wildtype fish and screening the offspring for mutations at 2 dpf using T7 endonuclease I (NEB) assay (for TALEN-injected fish ...
-
bioRxiv - Cell Biology 2020Quote: ... Founder fish were determined using T7E1 analysis (NEB), and positive hits were sequenced to determine exact mutation ...
-
bioRxiv - Genetics 2019Quote: ... Confirmation of the sequence of the alleles was achieved with the homozygous F2 fish using Phusion High-Fidelity DNA Polymerase (NEB) and Sanger sequencing.
-
bioRxiv - Genomics 2022Quote: For each of the two ligation-based protocols used for mouse sperm (Truseq, NEB Next), two replicate libraries for mouse sperm were prepared with the additional condition of an 18 hour ligation at 16°C for the ligation of the 3’ adapter in the attempt to increase ligation efficiency ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3-4 μg of sperm gDNA was dephosphorylated with 10 μl of rSAP (NEB, no. M0371S) and 16 μl of 10× rCutSmart buffer (NEB ...
-
bioRxiv - Cell Biology 2019Quote: ... Fluorescent probes were diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 µl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
-
bioRxiv - Genetics 2022Quote: ... Sperm small RNA was converted to cDNA using the NEBNext Small RNA Library Prep Kit (New England Biolabs; Ipswich, MA) and sequenced as 75nt single-end reads on the Illumina NextSeq 550 platform (San Diego ...
-
bioRxiv - Cell Biology 2021Quote: ... Fluorescent probes diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 μl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
-
bioRxiv - Developmental Biology 2020Quote: ... Probes were diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 µl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
-
bioRxiv - Physiology 2022Quote: ... where 250 nanograms of total RNA per fish were used with the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). RNA integrity number (RIN ...
-
bioRxiv - Microbiology 2021Quote: ... 40 μL of 10 μM FISH probes in hybridization buffer (10% dextran sulfate [Sigma], 2mM vanadyl ribonucleoside complexes [#514025, NEB] ...
-
bioRxiv - Developmental Biology 2024Quote: ... Probes were hybridised in FISH hybridization buffer (50% formamide, 20% dextran sulfate, 2X SSC, 1 μg/μL BSA (New England Biolabs), 10 mM vanadyl-ribonucleoside complex ...
-
bioRxiv - Developmental Biology 2020Quote: The lft2 exon regions were amplified by PCR from genomic DNA isolated from tail fin clips of a surface fish and individual F61 PA-CF male and females using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA) and the primers listed in Table S3 ...
-
bioRxiv - Synthetic Biology 2023Quote: DNA ladder: 1 kb DNA Ladder (NEB) for gel electrophoresis
-
bioRxiv - Biophysics 2020Quote: λ-DNA (NEB) was functionalized at one end by annealing with a biotinylated oligonucleotide/5Phos/AGGTCGCCGCCCTTTTT/Bio (IDT ...
-
bioRxiv - Cell Biology 2023Quote: ... 4.0 μg of the DNA were DNA-repaired and DNA-end-repaired using the NEBNext FFPE DNA Repair Mix (New England Biolabs; #M6630) and the NEBNext UltraII End Repair/dA-Tailing Module (New England Biolabs ...
-
bioRxiv - Cancer Biology 2019Quote: ... formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions ...
-
bioRxiv - Genomics 2020Quote: ... The DNA was repaired using T4 DNA polymerase (NEB) with dNTP (200 μM ...
-
bioRxiv - Microbiology 2021Quote: ... DNAs were ligated using T4 DNA ligase (NEB, USA); competent DH5α (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... NEBuilder HiFi DNA Assembly DNA Master Mix (E2621L, NEB) was used ...
-
bioRxiv - Microbiology 2022Quote: ... DNA ligase and DNA polymerase I (New England Biolabs). The product was used to construct a barcoded cDNA library using the Nextera XT system (Illumina ...
-
bioRxiv - Microbiology 2024Quote: ... DNA fragments were amplified using Phusion DNA polymerase (NEB) in reactions containing 10% DMSO and using GC buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Amplification of DNA fragments was performed with either Phusion DNA polymerase or LongAmp DNA polymerase (NEB).
-
bioRxiv - Cancer Biology 2021Quote: ... End-repairing of the sheared DNA (using T4 DNA polymerase, T4 DNA polynucleotide kinase, Klenow (all NEB)) was followed by dATP addition (Klenow exo- ...
-
bioRxiv - Molecular Biology 2023Quote: ... using gBlocks synthetic DNA fragments (Integrated DNA Technologies) and NEBuilder HiFi DNA assembly mix (New England Biolabs). HEK293 cells in 12-well plates were transfected with 1.6 μg of CircRNA Mini or mc2 plasmid encoding circMLLT2(3,4 ...
-
bioRxiv - Genomics 2020Quote: ΦX 174 virion DNA and Lambda DNA (New England BioLabs) were added to PCR reagents containing 1X Platinum Multiplex PCR Master Mix (Life Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA was end repaired using T4 DNA polymerase (NEB, M0203S), DNA polymerase I large fragment (NEB ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Lane M is DNA marker (NEB 1 Kb DNA Ladder), lane N shows the PCR product from negative control (ddH2O) ...
-
bioRxiv - Genomics 2019Quote: ... the nick-repaired DNA (NEBNext FFPE DNA Repair Mix, NEB) was sheared in a Covaris g-TUBE (Covaris ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was amplified using Phusion DNA polymerase (New England BioLabs) and SARS-CoV-2 specific primers surrounding the open reading frame for the spike protein ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was amplified using Phusion High-Fidelity DNA polymerase (NEB) and MiSeq (300+300 bp PE ...
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA was purified with Monarch PCR & DNA Cleanup Kit (NEB) and eluted in 20 µL of nuclease-free water ...
-
bioRxiv - Neuroscience 2021Quote: ... elegans genomic DNA using Phusion DNA polymerase (New England Biolabs). nCre expression constructs containing these promoters and the unc-54 3’UTR were created using Gateway cloning (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... DNA was end-repaired using DNA Repair Mix (NEB E6050L) and shotgun cloned into pWR2700 that had been cut with SmaI (NEB R0141L ...
-
bioRxiv - Molecular Biology 2022Quote: ... linearized vector DNA using T4 DNA ligase (New England Biolabs) following the manufacture’s protocol ...
-
bioRxiv - Genetics 2023Quote: ... DNA fragments were amplified using Q5 DNA polymerase (NEB 0491). PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the tagmented DNA was circularized with T4 DNA Ligase (NEB) and treated with a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and DNA ligation using T4 DNA Ligase (New England Biolabs).
-
bioRxiv - Cancer Biology 2024Quote: ... Digested DNA fragments were ligated using T4 DNA ligase (NEB) for 4 h at 16 °C ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA Fragments were repaired by T4 DNA polymerase (NEB, M0203), and T4 polynucleotide kinase (T4 PNK ...
-
bioRxiv - Biophysics 2021Quote: λ-DNA (N3011, NEB) was functionalized on one end with O6-benzylguanine (BG ...
-
bioRxiv - Cell Biology 2022Quote: ... DNA Assembly (NEB) was performed with four fragments ...
-
bioRxiv - Systems Biology 2020Quote: ... Prokaryotic DNA was enriched from the total hindgut DNA extract using NEBNext Microbiome DNA Enrichment Kit (NewEngland BioLabs). Following sequencing ...
-
bioRxiv - Biochemistry 2020Quote: ... The DNA was immediately purified using a DNA cleanup column (the Monarch PCR & DNA cleanup kit from NEB) and eluted with water ...
-
bioRxiv - Synthetic Biology 2021Quote: ... we used either a DNA ladder (Low Molecular Weight DNA Ladder or 1 kb Plus DNA Ladder, NEB) or an RNA ladder (RiboRuler Low Range or High Range RNA Ladder ...
-
Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Pre-adenylated DNA linker(DNA oligo purchase from IDT was adenylated using 5′ DNA Adenylation Kit (NEB,E2610S)) was ligated to the 3’-end of RNA in a 20 µl reaction (25% PEG8000 ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was isolated from blood (QiaAmp DNA Mini Blood Kit, Qiagen; or Monarch Genomic DNA purification kit, NEB) or from perfused organs homogenized (Fisher Bead Mill 4 ...