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Citations for New England Biolabs :
1 - 50 of 1540 citations for FABP2 I FABP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2019Quote: ... purified and then cleaved with Xho I and Bam HI (both NEB). After further purification ...
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bioRxiv - Synthetic Biology 2022Quote: ... sgRNA expression plasmids were constructed via digesting the sgRNA backbone plasmids that contained a human U6 promoter and a Cas-nuclease’s corresponding sgRNA scaffold sequences using BsmB I or Bbs I endonuclease (NEB) and then ligated the oligonucleotide duplexes into this cut backbone ...
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bioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Restriction digest of pMK-RQ plasmid DNA containing EtIMP1 was performed using Bam HI and Not I restriction enzymes (New England Biolabs). Restriction digest of pYD1-EtAMA1Cit plasmid was performed with the same restriction enzymes to remove the EtAMA1 insert but retain the citrine tag ...
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bioRxiv - Cancer Biology 2024Quote: ... by PCR amplification with the indicated primers (Key Resources table) followed by a digestion with Bam HI and Xba I enzymes (New England Biolabs) for 37°C 2 h ...
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bioRxiv - Neuroscience 2023Quote: Codon-optimized human TDP-43-His LIC-A constructs were transformed into T7 express (New England Biolabs, #C3029J) E ...
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MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browningbioRxiv - Cancer Biology 2023Quote: ... Miip CDS was amplified by PCR and cloned into LV17 (EF-1α-Luciferase-puro) shuttle vector (GenePharma, Shanghai, China) with restriction enzymes Not I and Bam HI (New England Biolabs, Ipswich, MA). For mouse Miip specific knock-down ...
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bioRxiv - Immunology 2021Quote: Restriction digest of pEX-K4 plasmid DNA containing EtAMA1Cit or was performed using Bam HI and Xho I (New England Biolabs, Ipswich, MA, USA) to extract tagged antigen coding sequences for cloning into pYD1 plasmid vector ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was performed in temperature cycles of 37°C for 2 min and 16°C for 5 min using the restriction enzyme Bsa I-HF® V2 and the Hi-T4™ DNA ligase (from New England Biolabs; Bioconcept, Allschwil, Switzerland), followed by a final 10 min ligation at 16°C ...
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bioRxiv - Cancer Biology 2020Quote: ... with Hi-Fi DNA builder (NEB). Primers used to amplify specific regions are described in (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... Exchange of V5/His to myc/His was performed using the Q5 site-directed mutagenesis kit (NEB #E0554) according to the manufacturer’s protocol resulting in pEF1-ZAP-S-myc/His and pEF1-ZAP-L-myc/His ...
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bioRxiv - Microbiology 2020Quote: ... were digested with BamH I and Xho I (NEB) (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNAse I (NEB) treated samples were cleaned up using RNAeasy cleanup kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... DNAase I (NEB) was then added to reactions to eliminate all remaining extracellular DNA ...
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bioRxiv - Microbiology 2020Quote: ... DNase I (NEB) treatment was performed on at least 5 µg RNA for 10 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... DNAse I (NEB), RNAse H (NEB)) ...
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bioRxiv - Biophysics 2023Quote: ... DNAse I (NEB), RNAse H (NEB)) ...
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bioRxiv - Genetics 2023Quote: ... Exonuclease I (NEB) and Plasmid-Safe ATP-dependent DNase (Lucigen ...
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bioRxiv - Neuroscience 2023Quote: ... DNase I (NEB), and murine RNase inhibitor (NEB ...
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bioRxiv - Genomics 2024Quote: ... Exonuclease I (NEB), Plasmid-Safe ATP-dependent DNase (Lucigen ...
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bioRxiv - Microbiology 2024Quote: ... DNase I (NEB) were added into the sample to remove possible extracellular DNA fibers.
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bioRxiv - Microbiology 2024Quote: ... DNase I (NEB) were added into the sample to remove possible extracellular DNA fibers.
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bioRxiv - Bioengineering 2023Quote: ... RNase I (NEB) and DNase I (NEB ...
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bioRxiv - Biophysics 2022Quote: ... 2018) and a C-terminal 6x His tag and cloned into the pET21B vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Biophysics 2022Quote: ... The DNA fragment was amplified by PCR by inserting a C-terminal 6x His tag and cloned into the pETDUET-1 vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Plant Biology 2022Quote: ... The samples were pooled by row into 8-strip tubes and excess primers were digested with the addition of 4.6 µL exonuclease I mix (2.5 µL of 10X Exonuclease I Buffer, 2.1 µL Exonuclease I; New England Biolabs) then incubated at 37□ for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Exonuclease I reaction mixture (1X Exo-I buffer, 1 U/µL Exo-I (New England Biolabs, cat# M0293L)) was pipetted into the device followed by an incubation at 37 °C for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Exonuclease I reaction mixture (1X Exo-I buffer, 1 U/µL Exo-I (New England Biolabs, cat# M0293L)) was pipetted into the device followed by an incubation at 37 °C for 45 min ...
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... pUAS vector was digested using Not I and Kpn I (NEB).
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bioRxiv - Microbiology 2020Quote: ... which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder ...
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bioRxiv - Genomics 2021Quote: ... a DNase-I digest was performed by using 1U DNase-I (NEB) in a total volume of 100 µl ...
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bioRxiv - Cell Biology 2019Quote: ... pAc 5.1 vector was digested by EcoR I and Not I (NEB). The amplified CTPS ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μL DNA Pol I and 20 U DNase I (NEB, U.K.), 1 mM each of dATP ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was cut with Hind III and Bam HI (NEB) for 1h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and His-MBP-ZFC3H1 onto an Amylose resin (NEB). After extensive washing ...
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bioRxiv - Immunology 2021Quote: ... were assembled using Hi-Fi DNA Assembly Mix (NEB). For the SFFV-Cas9 vector ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Hi-Fi assembly from New England BioLabs (NEB) and other basic molecular cloning approaches ...
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bioRxiv - Genomics 2021Quote: ... was then inserted by Hi-Fi assembly (NEB, E2621) using 0.025 pmols of vector and 0.05 pmols of the GFP amplicon ...
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bioRxiv - Cell Biology 2021Quote: ... using NEB Hi-Fi assembly mix (New England Biolabs). A mixture of the two guide plasmids (each at 100ng/µl ...
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bioRxiv - Developmental Biology 2023Quote: ... or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs) was used ...
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bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
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bioRxiv - Molecular Biology 2020Quote: ... DNase I (2uL NEB) was added to re-suspended chromatin-pellet and incubated at 37°C for 5-10 minutes to dissolve pellet ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Topoisomerase I (NEB).
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bioRxiv - Cell Biology 2022Quote: ... after DNase I (NEB) treatment of RNA ...
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bioRxiv - Microbiology 2019Quote: ... or DNase I (NEB) after elution and purified again using a second RNeasy mini kit isolation column ...
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bioRxiv - Molecular Biology 2021Quote: ... DNase I (NEB M0303) treatment was performed according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... and Exonuclease I (NEB). In vitro cleavage reactions were performed with 125 ng of exonuclease-treated circularized DNA ...