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Citations for New England Biolabs :
1 - 49 of 49 citations for Des Arg10 Kallidin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... de-phoshorylated (CIP, NEB, #M0290) and gel-purified ...
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bioRxiv - Molecular Biology 2020Quote: ... SmaI de-staining buffer (de-ionized water containing 1X cut smart buffer and 200 units of SmaI (NEB, #R01041S) in 500ul).
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bioRxiv - Systems Biology 2020Quote: ... de-phosphorylated with Antarctic phosphatase (New England Biolabs) and concentrated using PCR cleanup columns (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... de-phosphorylated using Alkaline Phosphatase (New England Biolabs) and purified by phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Neuroscience 2022Quote: ... All samples were de-phosphorylated using PNK (T4, NEB). The quality and concentration of samples was assessed by running an Agilent Small RNA chip (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was transformed into BL21(DE) cells (NEB), and a single colony was inoculated into 70 ml of LB and grown overnight in LB containing kanamycin ...
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bioRxiv - Microbiology 2022Quote: ... SlXyn10A was de-glycosylated with endo H (NEB, USA) according to the supplier’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein/lysate sample de-N-glycosylation using PNGase F (NEB), Endo S (NEB) ...
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bioRxiv - Genomics 2020Quote: ... 5′ de-capping with RNA 5′ pyrophosphohydrolase (RppH, NEB, M0356S), (3 ...
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bioRxiv - Genetics 2021Quote: ... De-crosslinking was performed by adding 50 μl proteinase K (NEB) and incubation at 65°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... De-capping was conducted to mediate adaptor ligation using RppH (NEB) followed by library preparation using NEBNext® Multiplex Small RNA Library Prep (Illumina) ...
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bioRxiv - Molecular Biology 2019Quote: ... de-capped mRNA using T4 RNA ligase I (New England Biolabs) and mRNA was purified using Dynabeads MyOne Streptavidin C1 beads (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... The resulting RNA was de-phosphorylated with Antarctic Phosphatase (New England Biolabs) at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... was de-phosphorylated with 2 μL of Calf Intestinal Phosphatase (NEB M0290S) for 1 hour with shaking (1250 rpm ...
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bioRxiv - Genomics 2021Quote: ... (iii) 5′ de-capping with RNA 5′ pyrophosphohydrolase (NEB, Ipswich, MA; M0356), (iv ...
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bioRxiv - Microbiology 2020Quote: ... Samples were de-glycosylated using EndoH (NEB, as suggested by the supplier).
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bioRxiv - Genomics 2022Quote: ... (iii) 5′ de-capping with RNA 5′ pyrophosphohydrolase (NEB, Ipswich, MA; M0356), (iv ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was then de-phosphorylated by incubating with T4 polynucleotide kinase (NEB) for 1 hr at 37° C ...
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bioRxiv - Plant Biology 2021Quote: ... and proteins were de-glycosylated with PNGase F (500,000 U/ml, New England Biolabs) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... the chamber was perfused with 500ul of the de-staining buffer (NEB, #M0303) and imaged at the indicated time points ...
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bioRxiv - Systems Biology 2023Quote: ... and de-phosphorylated with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA). The ligation was performed using NEB T4 ligase (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: Total RNA was (optionally, see text) de-phosphorylated using the Quick CIP (NEB M0525) (3 units/ µgRNA at 0.6 units/µL ...
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bioRxiv - Immunology 2022Quote: ... 125mM DTT) or further subjected to de-glycosylation with PNGase-F (NEB, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Digested plasmid was then de-phosphorylated with Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... and de-phosphorylating it with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA); 2 ...
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bioRxiv - Molecular Biology 2022Quote: Chromatin de-crosslinking was performed by adding 50 µl of 20mg/ml proteinase K (NEB) and 120 µl of 10% SDS and incubated at 55°C for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was cut and de-phosphorylated simultaneously using 1 U Shrimp Alkaline Phosphatase (NEB) and 20 U EcoRV-HF (NEB ...
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bioRxiv - Systems Biology 2023Quote: ... The backbone was de-phosphorylated with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA). All components were ligated together using NEB T4 Ligase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... a second bead binding was performed followed by a 5’ de-capping using RppH (NEB M0356S). The 5’ end of the transcript was phosphorylated using PNK (NEB M0201L ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 µg of HMW DNA were de-phosphorylated using Shrimp Alkaline Phosphatase (rSAP; NEB, M0371) for 30 min at 37°C followed by 5 min at 65°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting products were treated or not treated with mRNA de-capping enzyme (New England Biolabs) and then ligated to a 5’ adapter (Jin et al ...
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bioRxiv - Genomics 2024Quote: ... the cfDNA can be de-phosphorylated by adding 6 μl of Antarctic Phosphatase buffer 10x (NEB), 2 μl of Antarctic Phosphatase Enzyme (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and de-N-glycosylated using 500 U N-glycosidase F (PNGase F, New England Biolabs) for 12 h at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 8 μL of the de-phosphorylated DNA was incubated with 2 μL T4 Polynucleotide Kinase (NEB M0203S), 3 μL γ32P-dATP (Perkin Elmer) ...
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bioRxiv - Synthetic Biology 2020Quote: ... which was previously digested with the same enzymes and de-phosphorylated with Antarctic phosphatase (New England Biolabs). The templates for the inserts of libraries C45-I and II ...
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bioRxiv - Microbiology 2021Quote: ... or through synthesis of DNA fragments de novo followed by Gibson assembly (33) using NEBuilder HiFi (NEB). All clones were sequenced throughout the TBEV genome for verification.
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bioRxiv - Synthetic Biology 2021Quote: ... The plasmid DE was assembled via Gibson assembly (Gibson Assembly Master Mix of New England BioLabs, Inc.). The assembly was performed at equimolar concentrations of linearized plasmid MinDpUC57 and MinE DNA fragments for 1 hour at 50 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA or immunoprecipitated RNA (MIWI or MILI) was de-phosphorylated with Shrimp Alkaline Phosphatase (M0371, NEB) and end-labeled using T4 polynucleotide kinase (M0201 ...
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bioRxiv - Systems Biology 2019Quote: ... De-phosphorylated RNA was purified with an RNeasy MinElute spin column and re-phosphorylated with Polynucleotide Kinase (NEB). PNK-treated tRNAs were purified with an RNeasy MinElute spin column and ...
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bioRxiv - Molecular Biology 2020Quote: ... EcoRV de-staining buffer (deionized water containing 1X cut-smart buffer and 200 units of EcoRV (NEB, #R3195S) in 500ul) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNase I de-staining buffer (PBS containing 1X DNase buffer and 20 units of DNase I (NEB, #M0303) in 500ul) ...
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bioRxiv - Biochemistry 2023Quote: ... Antibody de-N-glycosylation was carried out by incubating the sample with PNGase F (New England Biolabs, Ipswich, MA) for 24 hrs at 37 OC (enzyme:substrate ratio ca ...
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bioRxiv - Genomics 2019Quote: ... These regions were mutagenized in order to introduce the de novo mutations of interest using the Q5 Site-Directed Mutagenesis kit (NEB) using non overlapping primers ...
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bioRxiv - Cancer Biology 2021Quote: ... chromatin from both IP and ‘Input’ samples were eluted and de-crosslinked at 65°C for 16 hours followed by DNA isolation by PCR and DNA cleanup kit (NEB). Enrichment of AR and MED1 at specified enhancers were quantified by qPCR using specific primers ...
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bioRxiv - Microbiology 2022Quote: ... Gene inserts were cloned into linearized pWSK29 (46) sequentially digested with the appropriate restriction endonucleases and de-phosphorylated with Shrimp Alkaline Phosphatase (New England Biolabs). Inserts were ligated into the linearized plasmid overnight at 16°C using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... 5′-Tru-Seq small RNA adapters were ligated on to the de-capped RNA with T4 RNA Ligase 1 (NEB) in the presence of ATP and cDNA synthesized following Illumina small RNA-seq protocol ...
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bioRxiv - Molecular Biology 2023Quote: The nascent RNAs bound to Streptavidin Dynabeads were 5’ de-capped by diluting in RNA 5’ Pyrophosphohydrolase (RppH) mix (NEB) and incubation at 37 °C for 45 minutes ...
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bioRxiv - Genomics 2019Quote: ... DNA libraries were prepared at the Fundación Parque Científico de Madrid (FPCM) using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs) and purified with Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... aeruginosa PAO1 genomic DNA and inserted into the expression vector pSEVA524 (Rubén de Dios, Eduardo Santero and Francisca Reyes-Ramírez, unpublished)78 by Gibson assembly (NEB Hifi Assembly, NEB Location). After conjugation with E ...