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Citations for New England Biolabs :
1 - 50 of 1555 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: MBP-Stwl antigen was purified from induced bacterial culture using Amylose Resin (NEB: E8021L). Briefly ...
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bioRxiv - Physiology 2021Quote: ... Enzymatic antigen retrieval with proteinase K (Biolabs, 2μg/mL) was used prior to incubation with antibodies ...
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bioRxiv - Pathology 2020Quote: ... Heat mediated antigen retrieval pretreatment using 10mM Sodium Citrate buffer (Vectors Biolabs) was used ...
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bioRxiv - Molecular Biology 2019Quote: ... Heat mediated antigen retrieval pretreatment using 10 mM Sodium Citrate buffer (Vectors Biolabs) were used ...
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bioRxiv - Immunology 2021Quote: Antigen binding fragment (Fab) was generated by incubating IgG with LysC (New England Biolabs, Cat# P8109S) at a ratio of 1 μg LysC per 10mg IgG at 37°C for 18hrs ...
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bioRxiv - Immunology 2020Quote: ... Antigen binding fragment (Fab) was generated by incubating IgG with LysC (New England Biolabs, Cat# P8109S) at a ratio of 1μg LysC per 10mg IgG at 37°C for 18hrs ...
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bioRxiv - Genomics 2019Quote: ... Purified RNA (500ng) was polyA purified using magnetic beads (NEB), fragmented and reverse transcribed using protoscript RT (NEB) ...
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bioRxiv - Genomics 2024Quote: ... gel-purified (NEB), and ligated to the annealed shRNA oligos ...
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bioRxiv - Immunology 2020Quote: ... Linker insertion and modifications in the antigen-bearing components were achieved using Q5 Site Directed Mutagenesis Kit (NEB). Custom DNA primers produced by Integrated DNA technologies (IDT) ...
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bioRxiv - Genetics 2023Quote: ... The BAC was purified purified using the Monarch Plasmid Miniprep Kit (NEB).
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bioRxiv - Synthetic Biology 2019Quote: ... purified (column-based NEB Monarch®PCR & DNA Cleanup Kit ...
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bioRxiv - Microbiology 2022Quote: ... gel purified (Monarch, NEB), and assembled NEBuilder HiFi DNA Assembly mix (NEB ...
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bioRxiv - Cell Biology 2019Quote: Purified Wss1 wild type (3.1 μM) was mixed with purified histone H3 (NEB) (3.3 μM ...
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bioRxiv - Biochemistry 2020Quote: ... pCSE2.6-hFc-XP or pCSE2.6-mFc-XP 68 where the respective single chain variable fragment of the antibodies or antigens were inserted by NcoI/NotI (NEB Biolabs) digestion ...
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bioRxiv - Biochemistry 2020Quote: ... pCSE2.6-hFc-XP or pCSE2.6-mFc-XP 68 where the respective single chain variable fragment of the antibodies or antigens were inserted by NcoI/NotI (NEB Biolabs) digestion ...
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bioRxiv - Bioengineering 2020Quote: ... the purified plasmids (New England Biolabs: Monarch® Plasmid Miniprep Kit ...
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bioRxiv - Cell Biology 2021Quote: ... column purified (T1030S, New England Biolabs) and then used for a restriction endonuclease digest ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were then purified (NEB, Monarch Nucleic Acid Purification Kit #20034198) ...
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bioRxiv - Cell Biology 2023Quote: All fragments were PCR purified (NEB), ligated using NEB HiFi assembly kit ...
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bioRxiv - Cell Biology 2023Quote: ... purified by gel extraction (NEB, T1020), and cloned into the CROPseq-Puro (Addgene ...
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bioRxiv - Immunology 2022Quote: ... the MPXVac-097 vaccine candidate construct encoding five monkeypox antigens in tandem was in vitro transcribed from linear DNA template using HiScribe T7 ARCA mRNA kit (NEB, Cat. No. E2060S) with 50% replacement of uridine by N1-methyl-pseudouridine (Tirlink ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with amylose (New England Biolabs #E8022L) columns ...
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bioRxiv - Molecular Biology 2021Quote: ... purified RNA was treated with RppH (NEB) to decap linear RNA and then was degraded using Terminator 5’-3’ exonuclease (Lucigen) ...
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bioRxiv - Developmental Biology 2020Quote: ... purified and digested with DpnI from NEB. Following another clean-up step ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... purified proprotein convertase furin (New England Biolabs) or protease trypsin (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... which was purified using amylose resin (NEB). The MBP-tag was cleaved by TEV protease treatment.
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bioRxiv - Cell Biology 2020Quote: ... and in vitro transcribed and purified (NEB HiScribe T7 High Yield RNA Synthesis Kit ...
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bioRxiv - Plant Biology 2021Quote: ... coli and purified using amylose resin (NEB). Target probes for MBP-CaWRKY40a upstream regions consisted of 150 bp fragments containing two predicted W-boxes that were amplified by PCR using Cy5-labelled primers and gel purified ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... the plasmids purified (NEB Monarch Plasmid miniprep) and sequenced to verify correct insertion using U6_Fw_seq primer (Table 1).
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bioRxiv - Developmental Biology 2020Quote: ... PCR amplicons were column purified (NEB, T1030) and used as a template for in vitro transcription reactions with a mMESSAGE mMACHINE® T7 Transcription Kit (Ambion ...
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bioRxiv - Cell Biology 2019Quote: ... Purified and HindIII-NotI (New England Biolabs) linearised genomic constructs were obtained by gel extraction (QIAquick Gel Extraction Kit ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were gel purified (NEB T1020L) and cloned into pMiniT 2.0 vector (NEB E1202S) ...
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bioRxiv - Immunology 2021Quote: ... purified proprotein convertase furin (New England Biolabs) was added at different concentrations (2-4 U/well ...
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bioRxiv - Developmental Biology 2021Quote: ... purified with Monarch RNA cleanup kit (NEB). The resulting sgRNA was used in an injection mix at 20 ng/µl per sgRNA ...
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bioRxiv - Genetics 2019Quote: ... purified with Monarch DNA Cleanup Columns (NEB) and digested again with BbsI to remove any carryover of undigested ...
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bioRxiv - Biochemistry 2023Quote: ... purified by affinity chromatography (amylose resin (NEB) or Strep-Tactin XT resin (IBA) ...
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bioRxiv - Developmental Biology 2024Quote: ... The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Biochemistry 2024Quote: ... and purified on amylose resin (NEB, E8021S) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Genetics 2021Quote: ... 1 μg of total Trizol-purified RNA purified was used for library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB) with libraries indexed in 12 cycles PCR ...
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bioRxiv - Cell Biology 2023Quote: ... His-MBP-Mis18BP120-130 was purified using the same lysis buffer containing 500 mM NaCl and purified using amylose resin (NEB). Proteins were then eluted by an elution buffer containing 10 mM Maltose.
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bioRxiv - Molecular Biology 2021Quote: ... purified DNA was digested with I-SceI (NEB) and AvrII (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... The purified amplicons were cloned into BsmBI (NEB)-digested CROPseq-Guide-Puro plasmids (Addgene #86708 ...
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bioRxiv - Microbiology 2019Quote: ... Purified RNA was treated DNAse (New England Biolabs) followed by EDTA ...
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bioRxiv - Genetics 2020Quote: ... and purified using a PCR purification kit (NEB).
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bioRxiv - Microbiology 2021Quote: ... purified using Monarch Gel Extraction Kit (NEB, USA) and ligated with SmaI-digested and dephosphorylated pUC19 vector using Blunt/TA Ligase Mastermix (NEB ...
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bioRxiv - Bioengineering 2021Quote: ... and purified using Monarch RNA cleanup kits (NEB).
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified with amylose resin (NEB) and eluted with 20 and 50 mM maltose (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... and purified on amylose columns (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... All proteins were purified using Amylose Resin (NEB) or HisPur Cobalt Resin (Thermo ...