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Citations for New England Biolabs :
1 - 50 of 8795 citations for Cow Serine protease HTRA2 mitochondrial HTRA2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The MVPI/MVPS mutants for Smac and HtrA2 as well as the active site mutant (S306A) for HtrA2 were cloned by Q5 mutagenesis-style (NEB, E0554S) linearization with primers containing the desired mutations ...
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bioRxiv - Cell Biology 2024Quote: ... Serine-35 mutations were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers are listed in the supplemental methods.
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bioRxiv - Microbiology 2023Quote: ... and the prototype serine endopeptidase trypsin (New England BioLabs), placed within a fluorescence plate reader (Spectra max Gemini XPS ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ... and aspartic acid 134 to serine using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Austria). The sequences were verified by both DNA and amino acid sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... Vector DNA was dephosphorylated using quick cow intestinal phosphatase (QCIP) and ligation reactions conducted using T7 DNA ligase (NEB). Purification of DNA products was done by PCR cleanup (E.Z.N.A Cycle Pure Kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gigas β/δ82Asn→Lys Hb mutant via site-directed mutagenesis on the Steller’s sea cow Hb expression vector by whole plasmid amplification using mutagenic primers and Phusion High-Fidelity DNA Polymerase (New England BioLabs), phosphorylation with T4 Polynucleotide Kinase (New England BioLabs) ...
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bioRxiv - Cell Biology 2023Quote: ... The C-KSR-GS construct replacing the vector-based linker (LELKLRILQS) by a glycine-serine rich linker (GGSSGGGGA) was generated by site directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, USA) of the C-KSR-EGFP plasmid ...
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bioRxiv - Microbiology 2022Quote: ... EE62/63AA and the 2_87 double serine phospho-mimetic SE53/57EE or “SS-EE”) using Phusion-II polymerase (New England Biolabs) and standard protocols ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Biochemistry 2023Quote: ... serine or aspartic acid were generated by engineering the corresponding constructs using Q5 site-directed mutagenesis (New England Biolabs, E0554). For this purpose ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... WT hNEMF was generated by mutating the serine at site 86 to an arginine using Q5 site-directed mutagenesis (NEB, E0554S). pBI-dsRED empty vector was cloned using annealing primers flanked by NheI and EcoRV ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Genomics 2021Quote: 1 µg of genomic DNA (nuclear + mitochondrial DNA) per 50 µl reactions was digested with 40 units of the recombinant restriction enzyme BamHI-HF (NEB) for 1 hour at 37°C in the presence of CutSmart buffer (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease K (P8107S, NEB). Antibodies used were ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA substrates for in vitro cleavage represent fragments of human mitochondrial DNA amplified by PCR in Q5® High-Fidelity 2X Master Mix (M0492L, NEB). As a template ...
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bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x protease inhibitor cocktail (NEB), 2 mM N-ethylmaleimide (NEM) ...
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bioRxiv - Biochemistry 2021Quote: ... or V8 protease (New England Biolabs) in 25 mM NH4HCO3 overnight at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... A TEV protease cut site preceding the 6x histidine tag was previously introduced using the Q5 Site-Directed Mutagenesis Kit from NEB and the GeneJET Plasmid Miniprep Kit from ThermoFisher ...
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bioRxiv - Biophysics 2021Quote: Time-resolved experiments with TEV protease were performed by adding 1 μL TEV protease (New England Biolabs) after the 3rd time point to an imaging well containing 100 μL protein sample ...
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bioRxiv - Biophysics 2023Quote: ... concentrated protein was supplemented with 100 U TEV-His7 protease in 1X TEV protease reaction buffer (NEB) and incubated at 30 °C with agitation for 3 hr to cleave the His6-tag ...
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bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
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bioRxiv - Biochemistry 2022Quote: ... 3µL of TEV protease (New England BioLabs) was added per 200µL of CrossMAb-ACE2 fusions at ∼2µM in 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... 2µL of TEV protease (New England BioLabs) was added per 200 µL of scFv-ACE2 fusions at ∼4µM in 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... 1X protease inhibitor cocktail (New England Biolabs)] ...
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bioRxiv - Biophysics 2021Quote: ... and was TEV-protease (50 units; NEB) cleaved overnight at 4°C to remove the APEX tag ...
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bioRxiv - Plant Biology 2021Quote: ... or TEV protease (P8112S, New England BioLabs) were used for the cleavage of constructs in the backbone of pMAL-c5x and for His-MBP-[TEV]-ADCK3 ...
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bioRxiv - Genomics 2019Quote: ... The embryos were treated with protease (1 µL of 25 µg/µL Qiagen Protease, 1 µL of 10x NEB Buffer 4 ...
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bioRxiv - Biophysics 2023Quote: ... was replaced with an HRV3C protease recognition site (LEVLFQGP) by site directed mutagenesis (Q5 site directed mutagenesis kit, New England Biolabs, Ipswich, MA). All amino acid substitutions were also introduced in GPΔTM and GPΔmuc via site- directed mutagenesis ...
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bioRxiv - Biochemistry 2020Quote: ... the resin was incubated with TEV Protease (NEB) for 3 h at room temperature ...
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bioRxiv - Genetics 2019Quote: ... and cleavage by TEV protease (New England Biolabs). The eluted chromatin was then used for a second purification step using Ni-NTA Agarose with nickel-charged affinity resin (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: ... The indicated amount of furin protease (NEB P8077) was added per reaction tube in a final volume of 20 μL ...
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bioRxiv - Genomics 2020Quote: ... 0.5% NP40 and 1x protease inhibitors (P8107S, NEB) on ice for 15 min ...
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bioRxiv - Biochemistry 2019Quote: ... and 3 protease inhibitor cocktail tablets (New England Biolabs) were added to the cell suspension ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 nL of protease mix was added (1x NEB CutSmart buffer ...
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bioRxiv - Cell Biology 2021Quote: ... samples were digested by protease K (New England Biolabs), eluted and cross-link-reversed ...
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bioRxiv - Genomics 2021Quote: ... 250 nL of protease mix was added (1× NEB CutSmart buffer ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of TEV protease (New England Biolabs, #P8112S) was added ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were cleaved with TEV protease (New England Biolabs) and further purified via reverse IMAC ...
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bioRxiv - Molecular Biology 2022Quote: For chitin-based sandwich ELISA 50 μl of chitin magnetic beads (New England Biolabs, Ipswich, MA, USA) were transferred into a 1.5 ml reaction tube ...
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bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
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bioRxiv - Biophysics 2021Quote: ... The desalted sample was then incubated with TEV protease (NEB) (50 units ...
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bioRxiv - Microbiology 2020Quote: ... the resin was treated with factor Xa protease (P8010L, NEB) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... 1X protease inhibitor cocktail ± lambda protein phosphatase (New England Biolabs) as per the manufacturer’s instructions and incubated at 30°C for one hour.
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bioRxiv - Neuroscience 2020Quote: TEV protease (TEV-P) was purchased from NEB (Cat#P8112S). PFFs were prepared as above and premixed 1:10 (for a final concentration of 15 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1 µL GluC protease (P8100S, NEB) at 1 mg/mL per mg of RH5ΔNL (1:1000 wt/wt) ...
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bioRxiv - Neuroscience 2022Quote: ... These solutions were added to 100 units of TEV protease (NEB) in aggregation buffer ...
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bioRxiv - Immunology 2020Quote: ... 0.09% Tween-20) containing 0.2 mg/ml protease K (NEB, P8107S) and incubating at 56°C for 1 hour ...
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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... Samples were deproteinized with 0.5 mg/ml of Protease K (NEB) and incubated for 1 hrs at 37 °C ...