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Citations for New England Biolabs :
1 - 50 of 10000+ citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Vector DNA was dephosphorylated using quick cow intestinal phosphatase (QCIP) and ligation reactions conducted using T7 DNA ligase (NEB). Purification of DNA products was done by PCR cleanup (E.Z.N.A Cycle Pure Kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gigas β/δ82Asn→Lys Hb mutant via site-directed mutagenesis on the Steller’s sea cow Hb expression vector by whole plasmid amplification using mutagenic primers and Phusion High-Fidelity DNA Polymerase (New England BioLabs), phosphorylation with T4 Polynucleotide Kinase (New England BioLabs) ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.25 μl 10 μM Illumina barcode (NEB-kit) and incubated ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 μM Illumina barcode (NEB-kit) to 23 μl of cDNA ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Plant Biology 2023Quote: ... and cleaned up with Monarch RNA Cleanup Kit (10 ug) (NEB). These cleaned RNA samples were sent to Novogene for poly-A tail enrichment mRNA library preparation and 150 bp paired end sequencing on Illumina NovaSeq (Novogene ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation was carried out in 10 µL using the PURExpress kit (NEB) according to the commercial protocol in the presence of a mix of 32P-radiolabeled cspA mRNA (200000 cpm/µL ...
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bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl 10 pM/μl and previously adenylated using the 5′ DNA adenylation kit (NEB, E2610S), and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL GlycoBuffer 2 (10 ×) (NEB), 10 μL 10% NP-40 (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL 10% NP-40 (NEB), 10 μL PNGase F (NEB ...
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bioRxiv - Molecular Biology 2022Quote: For chitin-based sandwich ELISA 50 μl of chitin magnetic beads (New England Biolabs, Ipswich, MA, USA) were transferred into a 1.5 ml reaction tube ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng DNA was used to prepare libraries using the NEBNext Ultra II DNA kit (NEB E7645S) as described with modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... and AAV9-based rep-cap plasmids incorporating individual heptameric peptides (EC1-10) (pACG2-[EC1-10], pACG2-QuadYF+TV-[EC1-10], and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng RNA were used for analysis with the LUNA one-step RT-qPCR kit (LUNA E3005L Biolabs). The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA (∼10 ng) was used to make libraries using the NEBNext Ultra II kit (New England Biolabs) and sequenced on the NextSeq 2000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from 10 hpf embryos using a Monarch total RNA miniprep kit (New England BioLabs Inc.) RNA was stored at −80°C until sample analysis and sequencing by the Molecular Evolution Core at Georgia Tech as previously reported(Johnson et al ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We performed a 10-cycle PCR using a Phusion PCR Kit according to the manufacturer’s protocol (New England Biolabs) with multiplexed primers and adjusted PCR products to 10 μM for sequencing on either an Illumina HiSeq2500 (125 bp paired-end ...
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bioRxiv - Genomics 2022Quote: ... 10 ng DNA was used to prepare sequencing libraries with the Ultra II DNA Library Prep kit (NEB #E7645S).
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription of 10 µl RNA was performed using LunaScript RT Supermix Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared from 10 ng of RNA using the NEBNext Ultra kit (New England BioLabs, Ipswich, MA, USA) and sequenced on an Illumina HiSeq 4000 with a 75 bp paired-end read ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Genomics 2019Quote: ... followed by adding 10 µL PNK (10 U/µL, NEB). The mixture was incubated for 30 min at 37 °C with shaking at 800 rpm ...
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bioRxiv - Genetics 2024Quote: ... 10 µL 10 mg/mL BSA (NEB cat no. B9000s), 840 μL DNA grade H2O (Invitrogen Waltham ...
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bioRxiv - Microbiology 2019Quote: ... and family 2 baby 10 month) we performed enrichment with the NEB Next Microbiome Enrichment Kit (New England Biolabs, www.neb.com) and sequenced both the pre-enrichment and post-enrichment samples ...
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bioRxiv - Bioengineering 2020Quote: ... We added 1 μL of the resulting DNase digestions to 10 μL RT-qPCR reactions (Luna One-Step Universal RT-qPCR Kit, New England Biolabs) containing 0.8 U/μL RNasin Plus (Promega ...
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bioRxiv - Systems Biology 2020Quote: The extracted plasmids were amplified using “lib-seq” primers in the Supplementary Table 6 for 10 cycles using Phusion PCR kit (NEB). Each 50 μL PCR reaction consisted of 6 μL of plasmid library ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of the diluted cDNA was used in 10 μl reaction mixture of Q5 Hot START DNA Polymerase kit (NEB) (2 μl of 5X buffer ...
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bioRxiv - Developmental Biology 2022Quote: miRNAs and total mRNA were extracted from a pool of 10 zebrafish hindbrains using the miRVana miRNA Isolation kit and gDNA removed using DNase1 (NEB). Reverse transcription was performed with either TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were carried out in 10 μl of the purified components from the PURExpress In Vitro Protein Synthesis Kit (E6800S/L, NEB) with the corresponding templates ...
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bioRxiv - Genomics 2020Quote: ... 25 uL of LNB buffer from LSK-109 kit (Oxford Nanopore Technologies) and 10 uL of ligase (New England Biolabs) and and 5 uL of adapter from LSK-109 kit was then added the reaction mixture and mixed by gentle tapping ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were then generated using 10 ng of DNA and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... RT-qPCR was performed on 10 ng total RNA using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005L). bin3 ...
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bioRxiv - Plant Biology 2023Quote: ... The reactions were completed in a final volume of 10 µl using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs), and relative gene expression analysis using the Livak method was per-formed 53 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng DNA was subjected to library preparation using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Libraries were then generated using 10 ng of DNA with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). The quality of the libraries was assessed with Agilent 2100 Bioanalyzer Instrument ...
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bioRxiv - Biochemistry 2023Quote: ... Parasites were collected by centrifugation (4000 xg, 10 min) and mRNA was extracted using a Quick-RNA Miniprep kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Double-barcoded lentiviral vectors were generated from FU-CGW by sequentially inserting matched 10-nucleotide barcodes into the PacI site distal to the HIV FLAP using the Quick Ligation Kit (New England Biolabs) and PCR amplification of the WPRE sequence and barcode with insertion into the ClaI sites proximal to the 3’ LTR by HiFi DNA Assembly (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... . Site-directed mutagenesis was performed on 10 ng pET28a(+)-HisPI31wt template with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Library amplification (10-12 PCR cycles) was carried out using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs), with IDT for Illumina UD Indexes (96x ...
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bioRxiv - Genomics 2021Quote: 10 μg genomic DNA from transfected mESCs were digested using 10 μl 10 U/μL NlaIII (NEB, #R0125S) in a 50 μL final volume for 3 hours at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2021Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2022Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2022Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mM MgCl2) and 10 U Klenow (exo-) polymerase (NEB, M0212S) and α-32P-dCTP (Perkin Elmer ...
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bioRxiv - Genetics 2021Quote: For CUT&RUN-sequencing libraries were made starting from 10 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) using the following PCR program ...