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Citations for New England Biolabs :
1 - 50 of 108 citations for Connexin 26 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... MBP-PUB25/26 were purified using Amylose Resin (NEB) by batch purification according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... An XK 26/60 column was packed with chitin resin (NEB), and equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... and pNJ-26 vector were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB), to generate the plasmid pSAG1:EGFP-DHFR-BAG1:mCherry ...
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bioRxiv - Molecular Biology 2023Quote: ... Three peptide phage libraries from NEB “New England Biolabs” ...
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bioRxiv - Genomics 2023Quote: ... Blocking was achieved using 1% BSA (NEB) and 0.1% Tween-20 in 1× PBS for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... BFP was amplified from Tol2-lyz:BFP [26] (a gift from Anna Huttenlocher) for HiFi cloning (NEB) into the Tol2-mfap4 vector cut open with SalI (F ...
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bioRxiv - Molecular Biology 2023Quote: Target sites of library-diverse were amplified by NEBNext Ultra II Q5 Master Mix (NEB, 26 cycles), and target sites from the TRIP library were amplified by GoTaq G2 Hot Start Green Master Mix (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Peptide-N-Glycosidase F (PNGase F; NEB, P0704) treatment of lysates was performed as described in the manufacturer’s directions ...
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bioRxiv - Genetics 2022Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3uM blocking oligo ...
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bioRxiv - Cancer Biology 2022Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3 µM blocking oligo ...
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bioRxiv - Molecular Biology 2021Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3uM blocking oligo ...
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bioRxiv - Microbiology 2021Quote: ... Peptide-N-glycosidase F (PNGase F) (New England BioLabs) was used to remove all N-linked oligosaccharides for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... or for peptide N-glycosidase F (PNGase-F, NEB) digestion using 500 units PNGase-F for 1,5h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... where the Emp24 transmembrane domain (residues 173-193) was replaced by 26 leucines using Gibson assembly (New England Biolabs). To construct pSP-FLAG-Cp ...
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bioRxiv - Plant Biology 2021Quote: ... The two sgRNA cassettes were digested out of pAtU6-26-SK using the restrict enzymes by SpeI and NheI (New England Biolabs) and subcloned into pCAMBIA1300-35S-Cas9 to generate pCAMBIA1300-AtU6-26-sgRNA1-AtU6-26-sgRNA2-35S-Cas9 ...
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bioRxiv - Plant Biology 2022Quote: ... The entry module pGG-B-AtU6-26-BRI1-2-C and pGG-A-AtU6-26-BRI1-3-B were generated by annealing oligos for each gRNA and ligating into BbsI-digested (New England Biolabs) Golden Gate entry vectors described in (66) ...
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bioRxiv - Neuroscience 2023Quote: ... pJFRC18-8XLexAop2-RGS2(Δ1-53)::CRY2(PHR)::T2A::CIBN::eGFP::CaaX and pJFRC18-8XLexAop2-RGS2(Δ1-53)::CRY2(PHR)(D387A)::T2A::CIBN::eGFP::CaaX were generated by cloning the PiGM cassette into pJFRC18-8XLexAop2-mCD8::GFP 26 cut with XhoI and XbaI (NEB). For zebrafish expression ...
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bioRxiv - Bioengineering 2024Quote: ... input genomic DNA was amplified in a 10-μl reaction for 26 cycles using NEBNext High-Fidelity 2x PCR Master Mix (NEB). PCR products were purified using Sera-Mag magnetic beads (Cytiva ...
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bioRxiv - Systems Biology 2023Quote: ... Whole metagenome sequencing libraries were prepared from 26 µL of DNA solution using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs). The DNA was purified and size selected to remove excess adaptors and adaptor dimers using Ampure XP beads (Beckman Coulter Life Sciences) ...
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bioRxiv - Bioengineering 2019Quote: ... 7-mer random peptide phage library (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Bioengineering 2019Quote: ... 7-mer random peptide phage library (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2022Quote: ... and tryptic peptides were generated using Trypsin-Ultra (P8101S, NEB) following the protocol described previously2 ...
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bioRxiv - Neuroscience 2022Quote: ... or peptide N-glycanase (PNGase, New England Biolabs, 1,000 units) for 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μL of peptide N-glycosidase F (New England Biolabs) were added ...
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bioRxiv - Microbiology 2021Quote: ... the SAG1 3’UTR was amplified from pNJ-26 and cloned into the tagging plasmid to replace DHFR 3’UTR by Gibson assembly (NEB, E5520S). BAG1-mCherry GCaMP6f reporter tachyzoites were co-transfected with 10 μg of pSAG1::CAS9-U6::sgDHFR 3’UTR and 2 μg of PCR amplified P2A-mTagBFP2-HXGPRT flanked with 40 bp homology regions ...
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bioRxiv - Cell Biology 2023Quote: ... full-length CTPS1 and CTPS2 cDNAs were obtained by PCR as previously described [26] using the Q5® High-Fidelity DNA Polymerase (New England Biolabs). For CTPS1 ...
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bioRxiv - Genomics 2022Quote: ... 500 nL of 5hmC-blocking mix (1 U T4-BGT (NEB, M0357L), 6x UDP-glucose ...
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bioRxiv - Immunology 2020Quote: ... The peptides were first digested with Endo H (New England Biolabs) to deplete oligomannose- and hybrid-type glycans and leave a single GlcNAc residue at the corresponding site ...
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bioRxiv - Bioengineering 2020Quote: ... reesei strain by peptide N-glycosidase F (PNGase F, NEB, P0704) (Wang et al ...
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bioRxiv - Cell Biology 2022Quote: ... or Peptide-N-Glycosidase F (PNGase F) (New England Biolabs, #P0704S) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... and Ph.D.-C7 peptide library (New England Biolabs, Ipswich, MA, USA) for the identification of AbOmpA binding phage ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA templates were generated by PCR (26) and used for synthesis of sgRNAs using NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). The sgRNAs purified using MEGAclear Kit (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: Peptide-N-glycosidase F (PNGase F) (New England Biolabs Inc., Cat. P0704S) was used for complete removal of N-linked oligosaccharides ...
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bioRxiv - Plant Biology 2020Quote: ... 1.5 μg of biotinylated histone peptides were incubated with streptavidin beads (NEB) in binding buffer (50 mM Tris-HCl 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... separated via self-cleaving T2A peptide was generated via Gibson Assembly (NEB). This cassette including a 5’ chimeric intron and 3’ poly adenylation signal sequence replaces the sequence of Pax7 exon 1 immediately 3’ of the ATG start codon ...
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bioRxiv - Immunology 2022Quote: ... N-glycans were enzymatically removed from the underlying peptides with PNGase F (NEB) overnight at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... A 7-mer random peptide library (E8100S, Ph.D. -7, New England Biolabs, USA) was panned overnight at 4°C on pooled human IgM adsorbed on polystyrene plates at a concentration of 0.1 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The Peptide-N-Glycosidase F (PNGase F) enzyme (New England Biolabs, catalog #: P0704L)-treated samples served as a control for unglycosylated α1 subunits ...
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bioRxiv - Immunology 2023Quote: ... N-glycans were enzymatically removed from the tryptic peptides with PNGase F (NEB) overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: Phage display experiments were performed using the PhD-12 peptide phage display kit (NEB). All steps involving the pipetting of phage-containing samples was done using filter tips (Rainin) ...
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bioRxiv - Biochemistry 2020Quote: ... tsetse saliva was treated with peptide-N-glycosidase A (PNGase A, New England Biolabs), which releases all N-linked glycans ...
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bioRxiv - Cell Biology 2019Quote: ... The PfGRP170 transit peptide PCR was digested with Nhe1 and AatII (New England Biolabs) and the GFP PCR was digested with AatII and BglII (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... Deglycosylation was performed with peptide-N-glycosidase F (PNGase F; New England Biolabs, USA) following the manufacturer’s instructions [28] ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabsⓇ Inc., MA, USA) was preferred in order to select aggregation inhibitory peptide selection ...
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bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was preferred ...
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bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was used ...
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bioRxiv - Biochemistry 2020Quote: Tsetse salivary proteins were treated with peptide-N-glycosidase F (PNGase F, New England Biolabs), which cleaves all N-linked glycans except those with an α-1,3 fucose modification of the chitobiose core [20] ...
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bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... to prevent exoIII digestion on the arrest peptide side and then with AvrII (NEB, R0174S) to allow for exoIII digestion towards the candidate protein ...