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Citations for New England Biolabs :
1 - 50 of 293 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Genetics 2020Quote: ... that when cleaved (NEB #R0539) allowed a desired target sequence to be inserted as part of the gRNA gene ...
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bioRxiv - Molecular Biology 2019Quote: ... or without 350 units of recombinant GSK3β (rabbit skeletal muscle) (New England BioLabs) and 1X hot kinase buffer (50mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... poly (ADP-ribose) polymerase (PARP) (both from Cell Signaling via New England Biolabs, Frankfurt, Germany), MYCN (abcam ...
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bioRxiv - Immunology 2021Quote: ... pAB270 was cleaved with NotI and SpeI (NEB). The fragment coding the SFFV promoter was amplified from GW175 (Kay Lab ...
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bioRxiv - Immunology 2021Quote: ... pAB27026 was cleaved using NotI and SpeI (NEB) and an saCas9 coding fragment ...
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bioRxiv - Microbiology 2023Quote: ... was cleaved with AfeI and BamHI endonucleases (NEB) to remove the DsRed gene ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was cleaved by XhoI (NEB, #R0146) and NotI (NEB ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were cleaved with TEV protease (New England Biolabs) and further purified via reverse IMAC ...
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bioRxiv - Biophysics 2019Quote: ... cleaved with the restriction enzymes AvrII and NotI (NEB, UK) and ligated using T4 DNA ligase (Promega ...
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bioRxiv - Genomics 2021Quote: ... The NMP lesion site was cleaved by hAAG (NEB, M0313S) and APE1 (NEB ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...
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bioRxiv - Bioengineering 2024Quote: ... Cleaved products were incubated with proteinase K (NEB, Ipswich, MA), A-tailed ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... Chromatin was cleaved using 450U HindIII restriction enzyme (BioLabs, Massachusetts, USA) at 37 °C overnight and diluted 15-fold in ligation buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... MBP was cleaved from LH using Factor Xa (New England Biolabs) at a w/w ratio of 1% Factor Xa:LH ...
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bioRxiv - Microbiology 2020Quote: ... cleaved with restriction enzymes (XbaI and EcoRI from New England BioLabs), and ligated into the preferred plasmid using Quick ligase (New England BioLabs) ...
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bioRxiv - Neuroscience 2019Quote: ... cleaved fragments were amplified by PCR using Q5 DNA polymerase (NEB) and cloned in pCR™4Blunt-TOPO® vector according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... and ligated to the cleaved backbone by T4 DNA ligase (NEB). The ligated products were transformed to E.coli competent cells (DH5α) ...
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bioRxiv - Biophysics 2023Quote: ... Mismatched duplexes were then cleaved by T7 Endonuclease I (NEB M0302S) and cleavage products were detected by gel electrophoresis ...
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bioRxiv - Biophysics 2024Quote: ... cleaved with BseRI using Gibson Assembly® Master Mix (NEB #E2611L) using the primers indicated in Table S1.
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bioRxiv - Immunology 2023Quote: ... via recombinant neuraminidase (NEB P0720L) as described by the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... and the barcode information cleaved using a Uracil-Specific Excision Reagent (NEB) targeting the 5d(U ...
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bioRxiv - Molecular Biology 2019Quote: ... purified and then cleaved with Xho I and Bam HI (both NEB). After further purification ...
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bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were cleaved using RNAse H (New England Biolabs, Ipswich, MA, USA) with LA121 as a specific primer for HIS3-tCYC1 ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently the 6xHIS tag was cleaved using the TEV Protease (NEB) by adding TEV enzyme assuming all protein isolate was 6xHIS tagged based on the activity of the TEV protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaved genomic DNA product was treated with NEBNext dA-tailing kit (NEB) and subjected to library preparation using nanopore adapter (ONT SQK-LSK109 ...
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bioRxiv - Biophysics 2024Quote: ... The RNA:DNA hybrid was then cleaved by RNase H (NEB catalogue # M0297L) to produce the final 22 nucleotide product ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved G protein was dephosphorylated by lambda protein phosphatase (NEB, Frankfurt, Germany), calf intestinal phosphatase (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.
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bioRxiv - Bioengineering 2022Quote: Recombinant Cas9 (New England Biolabs, USA) was used to generate a standard curve (20µM ...
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bioRxiv - Physiology 2022Quote: ... Recombinant CK2 was purchased from NEB. Recombinant TGFBR1 was purchased from Proqinase ...
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bioRxiv - Plant Biology 2021Quote: ... GST was cleaved using TEV protease (100 μL at 400 μM – New England Biolabs), and GST beads were added to eliminate GST and undigested proteins ...
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bioRxiv - Genomics 2022Quote: 500 ng to 1 μg gDNA were cleaved by SacI-HF (New England Biolabs) for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cleaved protein product was passed through Ni-NTA resin and amylose resin (E8021, NEB) to remove the TEV protease and HIS-MBP tag ...
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bioRxiv - Molecular Biology 2021Quote: ... SET8 recombinant enzyme (New England Biolabs # M0428S) was incubated with recombinant active PARP1 (Trevigen # 4668-100-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µl recombinant AsiSI endonuclease (10U/µL, NEB) was added to three biological replicates and incubated (2 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (rSAP, NEB) overnight and PCR purified (Qiagen QIAQuick PCR Purification Kit ...
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bioRxiv - Cell Biology 2023Quote: Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (all NEB) at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... 0.2 mg/mL recombinant albumin (NEB B9200S), 1 μM RT primer ...
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bioRxiv - Biochemistry 2021Quote: ... RBDs with flanking sequences were cleaved by using restriction enzymes XbaI and XhoI (NEB, USA) and ligated (T4 DNA ligase ...
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bioRxiv - Microbiology 2022Quote: ... of a pCAGGS vector backbone cleaved using EcoRV-HF and HindIII-HF (New England Biolabs) and a PCR fragment of a codon-optimized SARS-CoV-2 Wuhan spike protein with a C-terminal 19 amino acid deletion as described in (18) ...
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bioRxiv - Neuroscience 2022Quote: ... The amplified sequences were cleaved with the restriction endonucleases NcoI and NotI (New England Biolabs) and subsequently ligated into compatibly cleaved pHEN2 phagemid at a 3:1 (insert:phagemid ...
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bioRxiv - Microbiology 2022Quote: ... of a pCAGGS vector backbone cleaved using EcoRV-HF and HindIII-HF (New England Biolabs) and a PCR fragment encoding a codon-optimized SARS-CoV-2 Wuhan-Hu-1 spike protein (amplified from pCMV3-C-Myc ...
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bioRxiv - Microbiology 2023Quote: ... His/Strep-Tag was cleaved using enterokinase according to the protocol of the manufacturer (NEB) and GlnA3Mtwas immediately purified from the digestion mix by size-exclusion chromatography as directed by the resin manufacturer (GE-Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... Annealed products were ligated to the cleaved px330 plasmid using T4 DNA ligase (NEB, M0202T) and specific buffer (NEB ...