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Citations for New England Biolabs :
1 - 50 of 356 citations for Chromosome 11 Open Reading Frame 53 C11ORF53 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Adjustment of open reading frame following the splice acceptor was accomplished via Q5 (NEB) mutagenesis by inserting a single or two nucleotides (+1 or +2 ...
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bioRxiv - Plant Biology 2021Quote: ... reilianum ortholog open reading frame or promoters were amplified by Phusion DNA polymerase (NEB, Ipswich, USA) with primers (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... and deleting the eGFP open reading frame using site-directed mutagenesis with KLD enzyme mix (NEB). Ectopic complementation vectors were cloned onto pSEC1 (derivative of pK-PIM integrating vector ...
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bioRxiv - Neuroscience 2021Quote: ... The open reading frame was PCR-amplified (Phusion high-fidelity DNA polymerase, New England Biolabs Cat #M0530) using primers Irk1-F and Irk1-R (Supplemental Table 7) ...
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bioRxiv - Molecular Biology 2022Quote: WNT pathway selected open reading frames (ORFs) in pAVA were transformed into NEB Turbo (NEB C2986; discontinued) cells to obtain more than 20 million colonies split into two libraries of approximately 10 million each ...
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bioRxiv - Plant Biology 2020Quote: ... Products spanning the open reading frame of each gene were amplified with a high-fidelity Phusion® (NEB) DNA polymerase ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs encoding the Senp5 open reading frame (ORF) were amplified by Q5 High-Fidelity DNA Polymerase (NEB Japan) with primer sets based on the predicted sequences of the mouse Senp5 isoforms (GenBank accession numbers ...
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bioRxiv - Microbiology 2021Quote: ... we PCR amplified 600 bp DNA sequences flanking the open reading frame of each gene using Q5 DNA polymerase (New England Biolabs). We then cloned these sequences into EcoRI-XbaI digested pEXG2 by Gibson assembly using NEBuilder HiFi Assembly master mix (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: All dox-inducible factors were generated by cloning the open reading frame of each factor into the pMINI vector (NEB) and then restricted with EcoRI or MfeI and inserted into the FUW-TetO expression vector ...
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Physiology 2020Quote: ... A V5 tag was appended to the C terminus of the open reading frame using Phusion site-directed mutagenesis (New England Biolabs) to generate a V5 tagged version of mouse ANGPTL4 (pHS5) ...
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bioRxiv - Cell Biology 2019Quote: The donor constructs TagRFP-GAK and AP1-TagRFP were generated by cloning into the pUC19 vector with two ∼800-nucleotide fragments of human genomic DNA upstream and downstream of the start codon of GAK or the stop codon of AP1S1 and the open reading frame of TagRFP using the Gibson Assembly Master Mix (New England BioLabs). The open reading frames encoding TagRFP together with a flexible linker (5’-GGAGGATCCGGTGGATCTGGAGGTTCT-3’ ...
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bioRxiv - Immunology 2020Quote: ... The resulting expression vectors were confirmed for correct fusion protein open reading frames by sequencing and each was linearized with PmeI (New England Biolabs) and transformed into the P ...
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bioRxiv - Microbiology 2023Quote: ... and a 1848 bp fragment (containing the 1131 bp Pfcytb open reading frame) was amplified with primers (SI Appendix, Fig. S2A,B) and Phusion DNA polymerase (NEB). PCR product was verified by gel electrophoresis as single band of predicted size ...
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bioRxiv - Cell Biology 2023Quote: The open reading frame of SKR-1 was synthesized as a gBlock (IDT) and inserted into the pMAL-c6T vector (NEB) to express 6His-MBP (maltose-binding protein ...
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bioRxiv - Biochemistry 2023Quote: ... A V5 tag was then appended to the C terminus of the open reading frame using Phusion site-directed mutagenesis (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... and AcrIIA4 protein purification were generated by amplifying the backbone from the AcrVA1 plasmid and cloning in other open reading frames using HiFi assembly (NEB). E ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9 plasmids targeting sequences BLTP2 open reading frame were prepared by digesting pSpCas9(BB)-2A-Puro plasmid with BbsI (R3539S, NEB) and inserting duplexed primers caccGAGCCAAACACGGTTCCGGA and aaacTCCGGAACCGTGTTTGGCTC or caccGTGCAAGATCTGACGCCGAC and aaacGTCGGCGTCAGATCTTGCAC ...
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bioRxiv - Biophysics 2020Quote: ... SecM17 and NanoLuc sequences were synthesized (Integrated DNA Technologies) and inserted into the open reading frame with Gibson Assembly Master Mix (NEB E2611). All commercially available enzymes were purchased from NEB unless stated otherwise ...
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bioRxiv - Genomics 2023Quote: The open reading frames encoding distinct rBE candidates were amplified using Q5 High- Fidelity DNA Polymerase (New England Biolabs, Cat # M0491). The PCR primers were designed to produce PCR product flanked by ∼ 40 bp of sequence complementary to the target backbone vector ...
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bioRxiv - Genetics 2023Quote: ... was then linearized by Esp3i digestion and the HDAC4 PCR fragment was annealed downstream into the open reading frame with gibson assembly using NEB HiFi mastermix (NEB 2621L). After delivering the construct by lentivirus ...
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bioRxiv - Biochemistry 2019Quote: ... The gene was cloned into the first open reading frame of a pCDF-Duet vector using Gibson Assembly Kit (NEB, Ipswich, MA).[23 ...
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bioRxiv - Microbiology 2023Quote: ... 600 bp DNA sequences flanking the open reading frames of rmlC (PA5164) were PCR amplified using Q5 DNA polymerase (New England Biolabs, Ipswich, MA) (38) ...
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bioRxiv - Zoology 2024Quote: The complete open reading frames of AedaeCCHa1R and AedaeCCHa2R were amplified by PCR using Q5 high-fidelity DNA polymerase (New England Biolabs, Whitby, ON, Canada) with primers located upstream of the start codon and downstream of the stop codon (Supplement Table S1 ...
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bioRxiv - Plant Biology 2019Quote: ... Cysteine codon mutants and reading frame truncations were generated using Q5 site-directed mutagenesis (NEB). Transgenic Arabidopsis plant lines were generated by Agrobacterium tumefaciens-mediated floral dip transformation [34] and selected for homozygosity at T3 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were generated by PCR amplification of open reading frames (ORFs) in cDNA and ligation into a linearized pHAGEb vector using a Gibson assembly kit (New England Biolabs). dH5a or Stable competent E ...
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bioRxiv - Neuroscience 2021Quote: ... the Irk1WT and Irk1V306A open reading frames were amplified from pENTR-Irk1WT or pENTR-Irk1V306A by PCR (Phusion high-fidelity DNA polymerase, New England Biolabs Cat #M0530). Primers pAc5-Irk1-F and pAc5-Irk1-R included additional sequence for subsequent Gibson assembly cloning into the multi-cistronic vector ...
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bioRxiv - Bioengineering 2022Quote: ... Bacterial artificial chromosome (BAC) sequences from pBELOBAC11 (NEB) were amplified using forward (5’-TTAATTAAcgtcgaccaattctcatg ...
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bioRxiv - Genetics 2023Quote: ... In agar chromosome digestion with BamHI (NEB, R0136L) was used to release the entire rDNA locus (∼1.5 Mb to ∼5 Mb ...
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bioRxiv - Genomics 2022Quote: Method for chromosome digestion: prior to the BamHI (NEB CAT. R0136L) incubation ...
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bioRxiv - Bioengineering 2020Quote: ... we used the proof-reading enzyme Q5 from NEB, with its included buffer ...
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bioRxiv - Genetics 2020Quote: ... and fused in-frame by HiFi Assembly (NEB) to a blasticidin resistance marker amplified from pLentiCas9Blast (AddGene #52962) ...
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bioRxiv - Plant Biology 2021Quote: ... six PCRs were performed using Q5 proof-reading polymerase (NEB) with vector pUC57-R504 (kindly provided by Alison Huttly ...
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bioRxiv - Cell Biology 2023Quote: ... The FI was measured in all frames from NEB to AO and normalized to the frame of the NEB ...
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bioRxiv - Biochemistry 2023Quote: ... method linking intermediate wells.53 Concerted pathways were obtained by NEB directly from the reactants to the products ...
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bioRxiv - Systems Biology 2020Quote: ... Broad Range (11-245 kDa) (NEB P7712S) was loaded into an empty well.
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bioRxiv - Synthetic Biology 2021Quote: ... Broad Range (11-250 kDa) (NEB #P7718). Native gel electrophoresis of intact VLPs was run on a 1% w/v agarose mini gel (7×7 cm ...
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bioRxiv - Biophysics 2020Quote: ... and 3-biotin-GTP (NEB) and purified using MEGAclear ...
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bioRxiv - Biophysics 2021Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag of the VWF-dimer constructs in a bulk reaction in the presence of 5 μM sfp phosphopantetheinyl transferase53 and 10 mM MgCl2 at 37 °C for 60 min ...
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bioRxiv - Biophysics 2020Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag at the C-terminus of the fusion protein constructs in a 90 min bulk reaction in the presence of 4 µM sfp phosphopantetheinyl transferase63 and 100 mM MgCl2 at room temperature (≈ 22°C) ...
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bioRxiv - Cell Biology 2021Quote: ... PCR reactions were carried with the proof-reading Phusion DNA polymerase (NEB). Reaction conditions were according to the enzyme manufacturer's instructions with annealing temperatures chosen based on primers.
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bioRxiv - Molecular Biology 2023Quote: ... A gel lambda ladder PFG marker and yeast chromosome PFG marker (both from New England BioLabs, Inc.) were cast next to the gel plugs ...
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bioRxiv - Biophysics 2023Quote: Biotin-SNAP-mDia1 prepared by incubating purified SNAP-mDia1 with Benzylguanine-Biotin (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Open circular plasmid control was obtained by incubation with the Nt.BspQI nickase (New England Biolabs), and linear plasmid by cleavage with BamHI.
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bioRxiv - Microbiology 2019Quote: ... (S5 Table) and the proof-reading thermostable DNA polymerase Phusion (New England Biolabs). The library was cloned into the pCR4Blunt-TOPO vector (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were conducted with the proof-reading enzyme Phusion (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Second round nested-PCR reactions were performed using Phusion proof reading polymerase (NEB). A third round of PCR was performed with HotStar Taq DNA polymerase master mix (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... the biotin-Chd1p was prepared by mixing the SNAP-Chd1p and SNAP-biotin® (New England BioLabs) in a 1:2 molar ratio and incubated at room temperature for 30min ...
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bioRxiv - Microbiology 2023Quote: ... the closed 215-bp virS-containing DNA fragment was open by cleavage with NdeI (NEB, Germany). To this end ...
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bioRxiv - Systems Biology 2020Quote: ... Broad Range (11-245 kDa) (New England Biolabs, #P7712S). Lysates were run in 1x Tris-Glycine (Thermo ...