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Citations for New England Biolabs :
1 - 50 of 1180 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 135 ug/mL Prot K(NEB)) ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 ug of lamda DNA (NEB) or ssDNA M13 primer was labelled using the Ulysis Alexa Fluor 647 nucleic acid labeling kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... 1000 mU/ml micrococcal nuclease (NEB, M0247S) was applied to cells in a volume of 100μl per well and incubated at 37°C for 10 minutes ...
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bioRxiv - Genomics 2019Quote: ... D7 and D5 pre-annealed indexed adapters (2 ng and 200 ng, respectively) and T4 DNA ligase (400 U, NEB), and were incubated at 16°C overnight ...
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bioRxiv - Biochemistry 2022Quote: 30 uL of tagged Fluc-WT proteins were incubated with Factor Xa (NEW ENGLAND BioLabs, final concentration 67 ug/mL) for 2 hours or overnight on ice.
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bioRxiv - Genetics 2023Quote: ... we treated the glands with 0.1% Triton X-100 for 2 minutes prior to adding 100 ug/mL RNase A (NEB #T3018L) and performed a 1 hour incubation at RT (24 ...
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bioRxiv - Neuroscience 2023Quote: ... 1000 U/ml murine RNase inhibitor (New England Biolabs) added just before use ...
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bioRxiv - Microbiology 2022Quote: ... The following day swab samples were vortexed and spun down and 200 ul of the swab samples medium was combined with 16 ug/ml working concentration of TPCK-treated trypsin (New England Biolabs Ltd), 2x A/A/p/s antifungal/antibiotic solution (Wisent) ...
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bioRxiv - Physiology 2022Quote: ... 1000 U/ml MNase (catalog no. M0247S, New England Biolabs)) at a ratio of 1 ml of lysis buffer per 100 mg of cell pellet ...
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bioRxiv - Cell Biology 2022Quote: ... 1:100 or 1:1000 (stock 10 mg/mL, NEB) or with 1:10 Dnase I (stock 2 U/μL ...
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bioRxiv - Bioengineering 2019Quote: ... HBB-UG BAC with NotI (New England Biolabs, Cat. # R3189S) and all other BACs with the PI-SceI (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: 10 ug of protein extract were digested with PNGaseF (NEB) according to manufacturer instructions ...
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bioRxiv - Biophysics 2022Quote: ... Total of 60 ug of trypsin (mass spectrometry grade, NEB) was diluted in 25 mM NH4HCO3 and added to dried gel pieces (3x volume of the gel volume) ...
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bioRxiv - Biochemistry 2021Quote: ... equal quantities (5 ug) of untreated Ycf1 and lambda PP (NEB) treated samples were separated on two separate 10% SDS-PAGE gels ...
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bioRxiv - Plant Biology 2023Quote: ... and cleaned up with Monarch RNA Cleanup Kit (10 ug) (NEB). These cleaned RNA samples were sent to Novogene for poly-A tail enrichment mRNA library preparation and 150 bp paired end sequencing on Illumina NovaSeq (Novogene ...
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bioRxiv - Genomics 2020Quote: Dialyzed chromatin was utilized as input (1.5 ug) for methylation reactions with the non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock 2.5e4U/mL). Reactions were performed in a 200uL reaction with 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM ...
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bioRxiv - Genomics 2022Quote: ... followed by reverse-crosslinking with 2 ug/uL proteinase K (NEB, P8102), 1% SDS ...
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bioRxiv - Genomics 2021Quote: Purified RNA (5 ug) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described Smola et al. ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ug of RNA was reverse transcribed using the Lunascript Supermix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ug of DNA was incubated with 6U RNAse H (NEB, M0297) at 37 °C for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding.
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bioRxiv - Cell Biology 2020Quote: ... 100 ug plasmid were digested with 100 U Notl-HF and XbaI (NEB) for 7 h at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids (1 ug) were linearized using BamHI restriction enzyme (NEB, Ipswich, MA, USA), column-purified using the DNA Clean & Concentrator −5 Kit (Zymo Research Corporation ...
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bioRxiv - Genomics 2020Quote: DpnII digest:20 ug genomic DNA + 20 uL DpnII buffer (New England Biolabs) + 4 uL DpnII 9New England Biolabs ...
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bioRxiv - Genomics 2020Quote: DpnI digest: 10 ug genomic DNA + 10 uL CutSmart Buffer (New England Biolabs) + 2 uL DpnI (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... the culture filtrates from methanol-induced cultures were treated with PNGaseF (New England Biolabs), and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis ...
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bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
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bioRxiv - Microbiology 2021Quote: ... acnes lysates were incubated with 0.5 ug plasmid DNA together with different buffers (NEB) supplemented with or without ATP or SAM ...
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bioRxiv - Microbiology 2021Quote: ... primers that contained restriction sites for BamHI and XmaI or XbaI (Table S5, primers No. 9-16 and 95-98) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ug from each cell line was digested with 25 units EcoRI (New England Biolabs) in 100 ul total volume ...
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bioRxiv - Biochemistry 2022Quote: ... 2 ug of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA was digested with 20 U of RNase H (NEB #M0297S) at 37°C for 6 hours to serve as a negative control ...
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bioRxiv - Genomics 2022Quote: ... 3 ug of input DNA was dephosphorylated with Quick CIP (New England Biolabs, cat no M0508). Following enzyme inactivation with alkaline phosphatase ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were further quenched by the addition of 0.2 ug of RNAse A (New England Biolabs) and incubation at 37 °C for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug of RNA was reverse-transcribed using the WarmStart® RTx reverse transcriptase (New England BioLabs, M0380L) and random primer mix (New England BioLabs ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNA was isolated from 2.5 ug of total RNA using NEBnext Poly (A) magnetic isolation module (E7490S, NEB). The library was prepared as per the user’s manual using the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 ul PCR reactions were set up with approximately 1 ug cDNA and Phusion High-Fidelity DNA Polymerase (NEB) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mM sodium ascorbate before collecting them by scraping in 1 mL of the same solution with Murine RNase Inhibitor (1:1000, NEB). Cells were collected in a microcentrifuge tube ...
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bioRxiv - Cell Biology 2024Quote: ... each lysed by incubation in 1000 μL RIPA buffer + 2 mM PMSF + 60 μL PIC + 112.5 Kunitz Unit/mL DNase I (RNase-free, NEB M0303) + 2.5 mM MgCl2 (Sigma 5985-OP ...
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bioRxiv - Molecular Biology 2021Quote: ... Experiments comparing the impact of DNase I and RNase A on the migration of AbmR by SEC were completed by adding approximately 200 ug of purified AbmR 39S complexes to 20 units of DNase I (New England Biolabs) or 50 ug/ml of protease-free RNase A (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... approximately 1 ug of genomic DNA or WGA-DNA (See Suppl. Table 1) was dephosphorylated with Quick calf intestinal phosphatase (NEB) and CutSmart Buffer (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 8 ug of <200nt RNA per sample was mixed to an equal volume of 2x loading dye (NEB #B0363A) and incubated at 70°C for 5min ...
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bioRxiv - Cell Biology 2019Quote: ... 95°C for 10 minutes and equal amounts of lysates (180 ug) were treated with either 1uL of EndoH (NEB), PNGase F (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Poly(A)+ RNA transcript was isolated from 1 ug purified total RNA (RNA integrity number >9.0) with NEBNext poly(A) mRNA magnetic isolation module (New England Biolabs #E7490). RNA-seq libraries were prepared with NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolabs #E7420S ...
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bioRxiv - Molecular Biology 2023Quote: ... sgRNA amplicons were generated by PCR of 2 ug genomic DNA per 100 ul reaction with Q5 2X Hot Start Master Mix (M0494L, NEB) and enough reactions to maintain 1000x screening sgRNA representation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We isolated poly-adenylated RNA from 1 ug of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and constructed sequencing libraries with the NEBNext Ultra II RNA Library Prep kit (NEB #E7770 ...
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bioRxiv - Bioengineering 2019Quote: ... All BACs except DHFR BAC derived BACs were linearized before transfection: 2207K13-UG BAC with SgrAI (New England Biolabs, Cat. # R0603S), HBB-UG BAC with NotI (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: Approximately 1 ug of RNA was used as input to the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, catalog #E7490S), followed by NEB Ultra II RNA library prep kit for Illumina (New England Biolabs ...