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Citations for New England Biolabs :
1 - 50 of 377 citations for Caspase 9 CASP9 Antibody Pair since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Twenty nonfunctional 9 base pair barcodes “CellTags” were subcloned into pLentiV_Blast using Q5® Site-Directed Mutagenesis kit (New England Biolabs; catalog no. E0552) (Biddy et al. ...
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bioRxiv - Genomics 2021Quote: Purified RNA (9 μg) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described in Smola et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotide pairs were phosphorylated and annealed by mixing 100 pmol of each pair and 0.5 μL T4 PNK (NEB) then incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Restriction digestions were performed in a 60 ml volume with 1200 ng of DNA and 4–4.5 ml restriction enzyme(s) (Chr1 hotspot pair: NsiI, NcoI, and BglII or NcoI alone; Chr19 hotspot pair: BamHI or KpnI; BioLabs). Reactions were incubated overnight at 37 °C followed by an additional 3 h of incubation with a 1.5–2 ml of fresh restriction enzyme(s) ...
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bioRxiv - Plant Biology 2022Quote: ... Four vectors including one of the active 5′gRNA-pairs and one of the active 3′gRNA-pairs were digested by BglI (New England Biolabs) and ligated at once ...
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bioRxiv - Cancer Biology 2023Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single-cell libraries ...
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bioRxiv - Cancer Biology 2023Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single-cell libraries ...
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bioRxiv - Immunology 2021Quote: ... 9 U RNase Inhibitor (NEB) and 90 U Superscript II (Invitrogen) ...
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bioRxiv - Systems Biology 2019Quote: ... 9 µl RNase Buffer (NEB), 9 µl ATP (NEB ...
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bioRxiv - Systems Biology 2019Quote: ... 9 µl ATP (NEB, 10mM), 40 µl PEG 800 (50%) ...
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bioRxiv - Molecular Biology 2022Quote: ... A different Unique Dual Index Primer Pair (NEB, E6440S) was added to each sample and 11 cycles were used for the PCR enrichment ...
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bioRxiv - Developmental Biology 2021Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 9 units of T4 Polynucleotide Kinase (NEB) and 1 unit of Klenow fragment (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... 80ng of genomic DNA were fragmented to around 170bp followed by End Pair and A tailing with the End Pair mix (NEB Next Ultra DNA library preparation kit). After ligation of truncated TruSeq adapters with the NEB Next Ultra Ligation Module ...
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bioRxiv - Plant Biology 2020Quote: ... The enzyme pair HinfI and HpyCH4IV (New England Biolabs, Ipswich, USA) was selected based on the number of fragments ...
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bioRxiv - Genomics 2019Quote: ... and an NEB 100-base-pair (bp) ladder (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... was PCR amplified with mutagenic primer pairs using Q5 polymerase (NEB) (Supplementary Table S1) ...
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bioRxiv - Cell Biology 2019Quote: ... reactions using Random Primer 9 (New England Biolabs).
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bioRxiv - Cell Biology 2022Quote: ... and random 9-mer primers (New England Biolabs). Quantitative reverse transcriptase–PCR was performed using SYBR Green Master Mix (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... each forward/reverse oligo pair was annealed in T4 Ligation Buffer (NEB), with T4 PNK to phosphorylate the oligos ...
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bioRxiv - Genomics 2020Quote: Each oligos pair was further phosphorylated and annealed using T4 PNK (NEB) and T4 Ligation Buffer (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... Annealed sgRNA oligo pairs were phosphorylated with T4 PNK (NEB, USA #M0201S) and ligated with Quick Ligase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... specific i7 and i5 NEBNext index pairs (5uM) (New England Biolabs, U.S) were added to each sample and incubated as follows ...
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bioRxiv - Microbiology 2021Quote: ... 9 Neuraminidase A (P0722, NEB, 4 units/µg protein). 10 µg glycoprotein ...
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bioRxiv - Developmental Biology 2019Quote: ... Oligo pairs were annealed and phosphorylated by T4 Polynucleotide kinase enzyme (NEB, M0201S), chloroform extracted ...
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bioRxiv - Developmental Biology 2019Quote: ... Each oligo pair was annealed using T4 Polynucleotide Kinase (PNK) enzyme (NEB, M0201S) and then cloned into the sgRNA(MS2)_puro backbone (Addgene 73795 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR conditions were followed according to each primer pair as recommended by NEB using pCMV-SMAUG1-HisTEV2xFLAG as the template ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1 µl of a dual unique index multiplex primer pair (NEB; E6440) in a total reaction volume of 25 µl ...
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bioRxiv - Genetics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique index primer pairs) (E6440, NEB). We used 13 min fragmentation time and 9 cycles of PCR enrichment on 1000 ng of total RNA input determined by preliminary library preparation ...
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bioRxiv - Microbiology 2022Quote: ... These primer pairs were assembled by heat-denaturation and annealing in Buffer 3.1 (NEB), then cloned into BbsI-linearized pX458 (62 ...
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bioRxiv - Immunology 2023Quote: ... PCR was then performed on genomic DNA with relevant primer pair and enzyme (NEB) (see materials and methods chapter for PCR programme) ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche #08963851001) ...
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bioRxiv - Molecular Biology 2023Quote: ... two pairs of oligonucleotides (Suppl. Table 2) were phosphorylated and ligated into BsbI (NEB) digested pDG459 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... a pair of oligonucleotides (Suppl. Table 2) was phosphorylated and ligated into BsbI (NEB) digested pX459 and pX458 plasmids ...
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bioRxiv - Neuroscience 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001) ...
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bioRxiv - Microbiology 2020Quote: ... Purified RNAs were random primed using Random Primer 9 (NEB) by incubation at 65°C for 5 minutes followed by rapid cooling on ice ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each transcription reaction consisted of 9 mM rNTPs (NEB N0466), 10 mM Dithiothreitol (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... pMCM8-9 was dephosphorylated with λ-phosphatase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The parental caspase-1 plasmid (methylated) was digested using DpnI (NEB; Cat,# R0176S). Plasmids were transformed in E.coli DH5α competent cells (NEB ...
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bioRxiv - Immunology 2021Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector containing the constant part of IgG1 heavy or kappa/lambda light chain sequences respectively were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Genomics 2020Quote: ... each pair of oligos was first annealed and phosphorylated using T4 PNK (New England BioLabs) using the following program ...
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bioRxiv - Immunology 2020Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... a pair of overlapping PCR products were generated with Phusion high fidelity DNA polymerase (NEB) using pUAS-Abl:GFP (Fox and Peifer 2007 ...
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bioRxiv - Cell Biology 2020Quote: ... Each pair of oligos was annealed and phosphorylated with T4 PNK (NEB, Cat No. M0201S) in the presence of 10× T4 DNA ligase buffer in a thermocycler with the following parameters ...
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bioRxiv - Biophysics 2023Quote: ... each primer pair was annealed and consequently phosphorylated with T4-polynucleotide kinase (T4-PNK; NEB). Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo ...
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bioRxiv - Genomics 2023Quote: ... and 200 ng/µl Cas 9 protein (New England BioLabs, M0386T). Injected individuals were screened (see below genotyping procedure ...
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bioRxiv - Genetics 2019Quote: ... The sgRNA construct was made by inserting annealed pairs of oligonucleotides into Bsal (New England Biolabs) digested pDR274 (Addgene #42250 ...