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Citations for New England Biolabs :
1 - 50 of 1103 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... C-tailing was performed with Terminal Transferase (NEB #M0315) and 400-800 ng of end repaired gDNA using a 1:20 dCTP:ddCTP mixture (9.5 mM dCTP Millipore Sigma #3732738001 ...
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bioRxiv - Biochemistry 2020Quote: ... dmGemin2 was fused to a C-terminal Mxe intein (NEB) containing a hexahistidine tag ...
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bioRxiv - Neuroscience 2022Quote: ... was cloned into pDEST17 vector containing an N-terminal His10-Smt3-tag and a C-terminal SNAP-tag (New England BioLabs). The exact same purification scheme for DDX5(1-535)-SNAP was used to purify DDX5(1-483)-SNAP and the protein was flash frozen in medium salt storage buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: The full-length African killifish DDX5 was cloned into the pDEST17 vector containing an N-terminal His10-tag and a C-terminal SNAP-tag (New England BioLabs). E ...
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bioRxiv - Neuroscience 2022Quote: The full-length African killifish DDX5 was cloned into the pDEST17 vector containing an N-terminal His10-Smt3-tag and a C-terminal SNAP-tag (New England BioLabs). E ...
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bioRxiv - Neuroscience 2022Quote: ... was cloned into the pDEST17 vector containing an N-terminal His10-Smt3-tag and a C-terminal SNAP-tag (New England BioLabs). The same purification scheme was used to purify DDX5(1-535)-SNAP than DDX5-SNAP ...
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bioRxiv - Microbiology 2020Quote: ... the C-terminal domain was removed by digestion with Endoproteinase LysC (NEB) at a 1:800 LysC:substrate ratio by mass for 1-2h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The extension products were then C-tailed using terminal transferase (New England Biolabs). The resulting C-tailed extension products were then amplified using primers CDEP4816 and P1 or CDEP4816 and P3 to generate barcoded amplicon libraries for Illumina sequencing ...
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bioRxiv - Cell Biology 2019Quote: ... PACRGΔ1-69 was amplified from the GST-PACRGFL plasmid with PCR primers introduced BamHI (N-terminal) and XhoI (C-terminal) restriction sites for ligation in pGEX-6p1 with the T4 DNA ligase (NEB; Suppl. Figure S9). To generate the PACRG:MEIG1 co-expression vector the same protocol was performed as above using restriction enzyme cloning ...
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bioRxiv - Microbiology 2021Quote: ... The C-tailing reaction was performed by using terminal transferase (TdT) buffer (New England Biolabs), CoCl2 ...
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bioRxiv - Immunology 2022Quote: ... periplasmic expression vectors with C-terminal HA-His6 tags using Gibson cloning (New England Biolabs). Nanobodies were produced in Escherichia coli WK6 transformed with the respective expression vectors ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Biochemistry 2024Quote: Constructs bearing a C-terminal cMyc tag were cloned by site-directed mutagenesis (NEB, M0554S) using oligonucleotides as primers bearing a 5’-overhang encoding the inserted sequence ...
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bioRxiv - Cell Biology 2023Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology)6 sequence in-frame with the KRT5 sequence separated by a short peptide linker (DPAFLY) ...
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bioRxiv - Synthetic Biology 2019Quote: ... terminal transferase (NEB, M0315L) in 1X terminal transferase reaction buffer (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... and terminal transferase (NEB). Purified extended oligonucleotides were click-labelled with Cyanine5.5 or Cyanine7.5-alkyne (Lumiprobe) ...
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Biochemistry 2021Quote: ... and C-terminal additions) were assembled with the pcDNA5 backbone using Hi-Fi DNA assembly (NEB; E2621S).
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bioRxiv - Developmental Biology 2023Quote: ... A C-terminal HA tag was added using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For microinjection ...
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bioRxiv - Microbiology 2020Quote: ... The ClbI C-terminal GFP fusion was constructed using the Gibson Assembly kit (New England Biolabs, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously[40] ...
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bioRxiv - Biochemistry 2021Quote: ... The E PBM C-terminal mutation (DVLL>GGGG) was performed using the Q5 site-directed mutagenesis kit (NEB). The sequences corresponding to the PDZ domains were cloned into the pCMV GFP vector using EcoRI and XhoI sites.
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bioRxiv - Bioengineering 2021Quote: ... H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB), and pMHC molecules were expressed and refolded as described previously (19).
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bioRxiv - Biochemistry 2021Quote: ... also C-terminal) FT MYOC variants were generated by DNA assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB) by combining a PCR-amplified eGLuc2 product from a pcDNA3 MYOC eGLuc2 plasmid (originally described previously8) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 U Terminal Transferase (NEB) and ddH2O to a volume of 50 µl ...
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bioRxiv - Neuroscience 2023Quote: ... Chimeric constructs of both syntaxin isoforms were obtained using Gibson Assembly (NEB). For the viral infection the cDNA of mouse Stx1A (NM_016801.4) ...
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bioRxiv - Cell Biology 2020Quote: ... S141A ABHD11 and H296A ABHD11 with C-terminal eGFP tags or HA tags were created using NEBuilder HiFi (NEB). ABHD11 was also cloned into a transfection vector ...
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 404–561 with a C terminal His tag added was cloned into the pMAL-c5x vector (New England Biolabs), and were transformed in E ...
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bioRxiv - Microbiology 2022Quote: ... pCHYC-1 and a 500 bp ‘hfaE C-terminal PCR product were digested with HindIII-HF and KpnI (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... N- and C-terminal linkers of R-iLACCO0.1 were deleted using Q5 high-fidelity DNA polymerase (New England Biolabs) to provide variants with different linker length ...
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bioRxiv - Biophysics 2022Quote: ... The C-terminal Flag tag was introduced into pcDNA3.1-Spike-WT thanks to NEBuilder® HiFi DNA Assembly (NEB). The Flag tag was added during amplification of Spike-WT from pcDNA3.1-Spike-WT with the primers ...
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bioRxiv - Biochemistry 2024Quote: ... C-terminal Cx36 mutants were generated using the Q5 site directed mutagenesis kit (E0554S; New England Biolabs, Ipswitch, MA) and corresponding primer pairs introducing gaps into the Cx36 coding sequence ...
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bioRxiv - Cancer Biology 2021Quote: MaTAR20 isoforms were amplified by PCR using Phusion High-Fidelity DNA Polymerase (NEB) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The AcrIIA4 expression vector was modified to express AcrIIA11 with a C-terminal NLS and HA tags using the HiFi Assembly Kit (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-3xFLAG (C-terminal tag) using T4 DNA ligase (New England Biolabs). pcDNA4/TO-ORF24 202-752-3xFLAG (Addgene #138424 ...
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bioRxiv - Biochemistry 2022Quote: ... trcP or fragments of the trcP ORF were cloned into pHL100’s SmaI site with the 23 nt upstream sequence and the Flag tag sequence on the C-terminal using NEBuilder HiFi DNA Assembly Master Mix (NEB); subsequently ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by the insertion of a DNA fragment containing C-terminal 2×HA tag by NEBuilder HiFi DNA Assembly Master Mix (NEB). Finally ...
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bioRxiv - Biochemistry 2020Quote: ... The entire mDHFR ORF was PCR amplified from a mDHFR plasmid with a C-terminal AviTag fused to the mDHFR fragment using Gibson Assembly (NEB). The backbone contained a T7 promoter ...
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bioRxiv - Microbiology 2020Quote: ... A bacteria-codon optimized gBlock for the truncated protein was produced by IDT DNA Technologies and cloned into a pET28a bacterial expression with a C-terminal 6xHis tag using the NEBuilder Assembly Kit (New England Biolabs). Recombinant protein was expressed in BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... An expression clone for the full-length GFP-DdMyo7 with a C-terminal prenylation site (CAAX) was generated using Q5 mutagenesis (New England Biolabs) to add codons encoding the CTLL* prenylation motif from Dictyostelium RasG (UNIPROT ...
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bioRxiv - Biophysics 2022Quote: ... 2018) and a C-terminal 6x His tag and cloned into the pET21B vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Biochemistry 2022Quote: ... Constructs HsKHC-MDC and HsKHC-MDCL2-CaKip3 contained a cleavable C-terminal TEV-SNAP-6X-His tag (SNAP-tag from NEB). DARPin-D2 was ordered as a codon-optimized gene product from IDT and cloned into pET16b using Gibson Assembly ...
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bioRxiv - Biochemistry 2022Quote: ... The library was cloned into a pENTR1A plasmid backbone with a C-terminal YFP HA reporter tag using Gibson Assembly (HiFi DNA Assembly Cloning Kit, New England Biolabs, (NEB), Ipswich ...
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bioRxiv - Biophysics 2022Quote: ... The DNA fragment was amplified by PCR by inserting a C-terminal 6x His tag and cloned into the pETDUET-1 vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Microbiology 2021Quote: ... The repair templates used for the introduction of the C-terminal tags (YFP and SNAP) were amplified by PCR (Q5 polymerase, New England Biolabs) from template plasmids ...
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bioRxiv - Biophysics 2019Quote: ... and 15-25 μM of BG-oligonuculeotides were labeled with ∼ 1 μM myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer for 30 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... SMN-Gemin2 complex was produced by co-expression of SMN (residues 1-294) and Gemin2 (12-280) fused to a C-terminal Mxe intein (NEB) containing a hexahistidine tag ...
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bioRxiv - Biophysics 2021Quote: ... and 15–25 μM BG-oligonuculeotides were labeled with ∼1 μM myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... were fused to the fluorescent protein mNeonGreen with a C-terminal linker and cloned into pJBL044 under the constitutive promoter Pveg using Gibson assembly (New England Biolabs). The original pJBL044 plasmid was constructed using isothermal assembly from a fragment of pDR160 (Bose and Grossman 2011) ...