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Citations for New England Biolabs :
1 - 30 of 30 citations for BIgG SAP Goat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Genomics 2020Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Systems Biology 2019Quote: ... and shrimp alkaline phosphatase (SAP, NEB). The concentrations of the caps ...
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bioRxiv - Genomics 2024Quote: ... followed by dephosphorylation with Quick SAP (NEB). gRNAs were re-folded prior ABE8e:gRNA complexation ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were treated with Shrimp Alkaline Phosphatase (SAP; from NEB). In short ...
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bioRxiv - Genomics 2019Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 µl at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 μl at 37°C for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were processed according to the Exo-SAP protocol (NEB) and Sanger-sequenced by Azenta Life Services GeneWiz with hisC-ins-f as sequencing primer ...
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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bioRxiv - Biochemistry 2020Quote: ... the pTXB1 plasmid was double-digested with Nde-I and Sap-I (New England Biolabs, Ipswich, MA, USA) enzymes at the respective restriction sites to generate binding sites for insert ligation ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified products were cleaned using Exonuclease-Shrimp Alkaline Phosphatase (Exo-SAP) mixture (New England Biolabs, Ipswich, Massachusetts) and sequenced bidirectionally using BigDye v3.1 terminator kit in ABI 3500XL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were run by electrophoresis on a 2% agarose gel to ensure the absence of primer-dimers and PCR samples for each primer pair-opsin target were purified using Exonuclease (EXO) and Shrimp alkaline phosphatase (SAP) (NEB) prior to Sanger Sequencing to confirm gene-specific amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of DNA isolate was used for PCR (primers are in supplementary table 1.4) and PCR cleanup was performed using Exo-SAP (NEB, M0293, M0371). Sanger Sequencing reactions were prepared using (Thermo Fisher # 4337455) ...
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bioRxiv - Plant Biology 2021Quote: ... 500 ng of the SAP-treated RNA was treated with 12.5 units RNA 5’ Pyrophophohydrolase (RppH; New England BioLabs; Ipswitch, MA, USA) in 1X T4 RNA Ligase Buffer (New England BioLabs ...
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bioRxiv - Microbiology 2024Quote: PCR products of Asaia positive samples were cleaned up using Exonuclease I (Exo I) and Shrimp Alkaline Phosphate (Exo-SAP protocol) according to the protocol of New England Biolabs (NEB, MA, and USA) and sent for sequencing ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used were: Alexa-680 goat anti-mouse IgG and Alexa-800 goat anti-rabbit IgG (New England Biolabs). For DRGN cultures ...
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bioRxiv - Immunology 2021Quote: ... or an appropriate isotype-matched control (polyclonal goat isotype IgG, NEB) was added at a final concentration of 10 μg/ml and incubated overnight ...
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bioRxiv - Microbiology 2022Quote: ... goat polyclonal anti-UIS4 (LS Biolabs LS-C204260; IFA 1:1000); rabbit polyclonal anti-LISP2 (IFA ...
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bioRxiv - Plant Biology 2019Quote: ... Lysates pre-cleared for 15 minutes with 50µl goat anti-rabbit magnetic beads (NEB). Pre-cleared lysates were then incubated with either goat anti-rabbit magnetic beads only (mock IP ...
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bioRxiv - Microbiology 2019Quote: ... followed by Alexa Fluor 488 goat anti-rabbit secondary antibody (New England Biolabs, UK) for 1h ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies used for targeted depletion were prepared using goat anti-rabbit magnetic beads (NEB, S1432S) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... the membrane was incubated with HRP-conjugated secondary antibody (goat anti-rabbit HRP, BioLabs 7074P2) in 3% BSA in PBS-T shaking for 1-2 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C followed by Alexa Fluor 488 goat anti-rabbit secondary antibody (New England Biolabs, UK) for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatants were immunoprecipitated overnight with 40 µL of precoated anti-IgG magnetic beads (goat anti-rabbit IgG magnetic beads, NEB) previously incubated with the antibody of interest for 4 h at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed neurons were then permeabilized and blocked simultaneously (2% normal goat serum, 5425S, New England Biolabs, and 0.1% Triton X-100) before incubation in primary antibody solutions overnight and subsequent incubation with secondary antibodies the following day.
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bioRxiv - Evolutionary Biology 2023Quote: ... before being incubated in 5% normal goat serum dissolved in PBS-TX (NGS-PBS-TX; NGS; New England BioLabs, Hitchin, Hertfordshire, UK) for a minimum of one hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... with rotation for 1 hour at room temperature followed by further incubation for 1 hour with the addition of 15 μl of goat anti-mouse IgG magnetic beads (New England Biolabs, Ipswich, MA, USA). The magnetic beads in the supernatant were collected on a magnetic stand and washed 2 times with PBS ...