Labshake search
Citations for New England Biolabs :
1 - 50 of 149 citations for Argininosuccinic Acid Barium Salt 2H2O Arginine 13C6 99%; 15N4 99% 90%+ Cp since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... the 1:99 target:background sample was digested with FspEI (New England Biolabs) according to the manufacturers protocol (incubation at 37°C for 90 minute ...
-
bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
-
bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
-
bioRxiv - Genomics 2019Quote: ... Site-directed mutagenesis for Exd and Hth was performed via amplification of the original plasmid with primers harboring single amino acid replacements (arginine to alanine) using Taq-polymerase (NEB). Double and triple mutations were generated consecutively ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... CPS mutant plasmids were constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
-
bioRxiv - Plant Biology 2022Quote: ... we linearized plasmid pL4440-CP in independent reactions with BglII and HindIII (NEB). Once linearized ...
-
bioRxiv - Bioengineering 2021Quote: ... 90 nM of SpCas9 protein (NEB), NEB buffer 3.1 (NEB ...
-
bioRxiv - Cell Biology 2023Quote: ... 90 nM of Cas9 nuclease (NEB), Cas9 nuclease buffer (NEB ...
-
bioRxiv - Genomics 2024Quote: ... 90 nM of SpCas9 protein (NEB), NEB buffer 3.1 (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... 90 nM of SpCas9 protein (New England Biolabs), Cas9 nuclease buffer (New England Biolabs ...
-
bioRxiv - Biochemistry 2022Quote: ... 90 μL dH2O and +/− 2500 U PNGase F (NEB) was added to each tube and incubated at 37 °C for 1.5 h ...
-
bioRxiv - Genetics 2020Quote: ... 90 nM of SpCas9 protein (#M0386, New England Biolabs), NEB buffer 3.1 ...
-
bioRxiv - Immunology 2021Quote: ... 90 nM of EnGen® Sau Cas9 protein (NEB), NEB buffer 3.1 (NEB ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were diluted in 90 μl Elution Buffer (NEB), mixed with 120 μl AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Genomics 2019Quote: ... and SalI-HF (37 °C, 90 min, New England Biolabs). The fragments were column-purified ...
-
bioRxiv - Biophysics 2023Quote: ... Purified SNAP-CP was incubated with 5x excess of SNAP-surface-549 dye or SNAP-surface-649 dye (New England Biolabs, USA) overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 8 U/μl Salt-T4 DNA ligase (NEB #M0467)) ...
-
bioRxiv - Biochemistry 2022Quote: ... A 25 μg aliquot of CP-treated CTDNA was diluted to 100 μL of 1X nucleoside digestion mix reaction buffer (NEB, Ipswich, MA) at pH 5.4 and treated with 2.5 μL NEB nucleoside digestion mix (1 μL or 1 U per 10 μg CTDNA ...
-
bioRxiv - Systems Biology 2021Quote: ... for 90 minutes and then ligated sequencing adaptors with T4 ligase (NEB) for 30 min ...
-
Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... WT hNEMF was generated by mutating the serine at site 86 to an arginine using Q5 site-directed mutagenesis (NEB, E0554S). pBI-dsRED empty vector was cloned using annealing primers flanked by NheI and EcoRV ...
-
bioRxiv - Microbiology 2020Quote: ... in 0.5 x TBE for 60-90 min alongside 100 bp ladder (NEB). Gels were stained for 30 minutes in ethidium bromide solution before imaging under UV-light.
-
bioRxiv - Biochemistry 2022Quote: ... reactions to generate catalytic arginine point mutants for AIM18 and AIM46 were performed according to the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Table S2). SDM reactions were transformed into E ...
-
bioRxiv - Genomics 2023Quote: ... RNAs were 5’dephosporylated through 90 minutes incubation in total with thermostable QuickCIP (NEB) in which the samples were briefly heated to 75°C and quickly chilled on ice at the 60 minutes mark ...
-
bioRxiv - Molecular Biology 2019Quote: m7G-capped RNA substrates 90 to 1400 nucleotides long were generated by T7 RNA polymerase (NEB) transcription of different DNA templates generated from an FLuc plasmid ...
-
bioRxiv - Systems Biology 2019Quote: ... Biotinylation was performed in a 90 µl reaction with 6 U of T4 ssRNA Ligase (NEB), 4 µl Biotinylated Cytidine (Thermo Fisher ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs). After DNA pooling ...
-
bioRxiv - Biochemistry 2023Quote: ... formic acid was purchased from Biolabs ltd ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl genomic DNA (input 90 ng) from naïve hESCs were digested with LpnPI (New England Biolabs) generating 32 bp fragments around the fully methylated recognition site containing a CpG ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were treated for 90 min with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Biophysics 2022Quote: Nucleosome reconstitution was performed by salt dialysis on biotinylated DNA (pKYB1 vector; New England Biolabs) without any nucleosome positioning sequence ...
-
bioRxiv - Microbiology 2021Quote: ... RNase I (6xHis) or refolded RNase I-ACE2NTD (6xHis) in a high salt buffer (NEB buffer 3 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Nucleosomes were reconstituted with a salt dilution method according to manufacturer’s instructions (New England BioLabs) with a slight modification ...
-
bioRxiv - Biochemistry 2019Quote: ... transferred at 300 mA for 90 minutes and probed overnight with α-MBP (1:10000, New England BioLabs) or α-ORC (1:1000 ...
-
bioRxiv - Systems Biology 2024Quote: ... PCR products (90 bp) were spin-purified and 300 ng was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 2 h at 37°C (no heat kill ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... The resulting library was pooled into 12 separate 60 amino acid long sub-libraries (amino acids 1-60, 61-120 etc.) and combined via Gibson Assembly (NEB) with a linearized p414ADHΔter Hsp90 destination vector ...
-
bioRxiv - Genomics 2019Quote: ... fragments compatible with Illumina NGS were generated digesting the products of rolling circle amplification with BtsαI (55 °C, 90 min, New England Biolabs) and SalI-HF (37 °C ...
-
bioRxiv - Neuroscience 2022Quote: Libraries were prepared from 90-ng of RNA from each sample using the NEBNext Ultra kit (New England BioLabs). Samples were sequenced on an Illumina HiSeq 4000 with a 75 bp paired-end read ...
-
bioRxiv - Developmental Biology 2021Quote: ... Chromatin was digested with 100 U of MboI restriction enzyme (90 min at 37C with rotation; New England Biolabs) and the enzyme was heat-inactivated at 62C for 20 min ...
-
bioRxiv - Biophysics 2020Quote: ... Each mix was incubated at 90°C for 5 min and annealed in 1x T4 DNA Ligase Reaction Buffer (B0202S; NEB) by gradual cooling ...
-
bioRxiv - Biophysics 2019Quote: ... This fragment was PCR-amplified with the oligonucleotides 89.F lambda 40002 XhoI and 90.R lambda 45263 ApaI using Lambda DNA (NEB) as a template ...
-
bioRxiv - Genetics 2022Quote: NGS libraries were generated by amplifying 12 μl of the eluted CUT&Tag DNA fragments with i5 and i7 barcoded HPLC-grade primers (90) (Suppl. Table 4) with NEBNextHiFi 2x PCR Master Mix (New England BioLabs) on a thermocycler with the following program ...
-
bioRxiv - Plant Biology 2023Quote: ... At least 50 μg of total RNA was randomly fragmented for 125 s at 90°C to approximately 200 nt using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Fragmented RNA was precipitated with 4 μl of GlycoBlue (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2023Quote: ... and size-selected for 50-90 nt fragments for constructing a sequencing library using Universal miRNA Cloning Linker (NEB; S1315S) and the RNA-Seq library construction procedures described above ...
-
bioRxiv - Cancer Biology 2023Quote: mRNA was isolated from 6-well plates showing a cell confluency of ∼90% using the Monarch Total RNA Miniprep kit (NEB). siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... pH = 8 for 16 hours at 56°C followed by 30 minutes at 90°C and an additional digest of 4 units of beta-agarase (M0392S, New England BioLabs) for 1 hour at 65°C to ensure full agarose breakdown ...
-
bioRxiv - Cell Biology 2023Quote: ... gel extracted using Monarch Nucleic Acid Purification Kits (NEB) and submitted for Sanger Sequencing ...
-
bioRxiv - Cell Biology 2023Quote: ... Free nucleotides were purchased as 100mM stocks of dATP or dUTP as sodium salts in ultrapure water (NEB) and stored at -20°C ...
-
bioRxiv - Genetics 2021Quote: ... Nuclei were pelleted at 500 rcf at 4°C for 5 minutes and resuspended in 90 uL 1X Cutsmart Buffer (NEB B7204S). 10 uL of 10U/uL AluI restriction enzyme (NEB R0137S ...
-
bioRxiv - Neuroscience 2020Quote: ... The beads were washed three times with IP buffer and resuspended in 90 ul of IP buffer with proteinase K (NEB, P8107S) for 30 mins at 37°C ...
-
bioRxiv - Biophysics 2022Quote: ... the reaction was diluted to 90 µl and was supplemented with 10 µl DNAse I buffer and 2 µl DNAse I enzyme (NEB #M0303S) and incubated for 15 minutes at 37□ C to degrade the DNA template ...