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Citations for New England Biolabs :
1 - 50 of 3526 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... a catalytic subunit of protein kinase A (PKA Cα, New England Biolabs); Ca2+/calmodulin-dependent protein kinase II (CaMKII ...
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Biochemistry 2023Quote: Trx-linker concatemers (1 mg/mL) were incubated with 50,000 units of the catalytic subunit of cAMP-dependent protein kinase (PKA) (NEB)—which recognizes the RRAS motif within the central linker of the Trx-linker nonamer—in protein kinase buffer (50 mM Tris HCl ...
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bioRxiv - Neuroscience 2024Quote: ... 4.1 μl of PLPPR3 ICD (final concentration 0.075 mg/ml) was mixed with 0.2 μl purified PKA catalytic subunit (final concentration 20 000 Units; #P6000S, Biolabs), 0.8 μl phosphorylation buffer (final concentration of 25 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 200 µM ATP with ot without 1 µl of recombinant PKA catalytic subunit (PKAc; New England Biolabs) in manufacturer-supplied reaction buffer at 30° C for 30min with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... pombe mRNA decapping enzyme (NEB #M0608S) were used as negative and positive controls ...
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bioRxiv - Neuroscience 2022Quote: ... The non-15N 4R tau used for small-scale chemical in vitro crosslinking was phosphorylated using cAMP-dependent Protein Kinase (PKA, catalytic subunit, New England Biolabs #P6000S) according to manufacturer guidelines (25uL reaction at 30°C for 2 hours with 200 μM ATP ...
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bioRxiv - Cancer Biology 2024Quote: ... 2ul mRNA decapping enzyme (New England Biolabs M0608S), and 1ul RNase inhibitor and incubating at 37°C for 30 minutes ...
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bioRxiv - Genomics 2019Quote: ... and 0.34 μl mRNA Second Strand Synthesis enzyme (NEB) were then added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... Further end treatment was performed using mRNA Decapping Enzyme (NEB) pre-mixed with its buffer directly into the PNK reaction and incubating at 37 C for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... 0.66 μL mRNA Second Strand Synthesis buffer and 0.34 µL mRNA Second Strand Synthesis enzyme (NEB, E6111L) were then added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... purified RNA was incubated with mRNA decapping enzyme and buffer (NEB) for 1hr at 37℃ ...
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bioRxiv - Immunology 2022Quote: ... cleavage rates were transformed into standardized catalytic activities (1 “eqU” = equivalent to the catalytic activity of 1 U RNase HII (NEB ®) under standard conditions) ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.75 μl of second strand mix (0.5 μl of mRNA second strand synthesis buffer and 0.25 μL of mRNA second strand synthesis enzyme, New England Biolabs) were added to each well ...
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bioRxiv - Genomics 2023Quote: ... and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs, E6111L) in each well ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs, E6111L). The plates were incubated at 16°C for 3 hours ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of mRNA for TRAP complex subunits were estimated by RT-qPCR using Luna Universal One-Step RT-qPCR Kit (NEB) following total RNA isolation with RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Neuroscience 2019Quote: ... Editing activity was validated by the T7E1 assay (NEB) in rat C6 glioma cells (ATCC) ...
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bioRxiv - Immunology 2020Quote: ... Editing efficiency was estimated by T7 endonuclease I (NEB) assay according to the manufacturer’s instructions using the following primers ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 µg of total RNA was decapped with mRNA decapping enzyme (New England Biolabs) for 1 hour at 37 ºC ...
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bioRxiv - Biophysics 2023Quote: ... The mRNAs were capped using the vaccinia virus capping enzyme kit (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... a phage editing strain consisting of dh10b (NEB, Intact Genomics) containing a homologous recombination vector (pBA787-pBA792 ...
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bioRxiv - Molecular Biology 2019Quote: ... All mRNAs were capped using the Vaccinia enzyme m7G capping system (New England Biolabs M2080S). All mRNA samples were analyzed for purity and cap content by capillary electrophoresis.
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bioRxiv - Cell Biology 2022Quote: ... Editing efficiency was assessed using the EnGen Mutation Detection Kit (NEB) (E3321S ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting products were treated or not treated with mRNA de-capping enzyme (New England Biolabs) and then ligated to a 5’ adapter (Jin et al ...
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bioRxiv - Biochemistry 2023Quote: ... The HBB 5’ UTR containing mRNA was then capped using Vaccinia Capping enzyme (New England Biolabs) and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs) ...
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bioRxiv - Bioengineering 2024Quote: ... The total RNA was isolated at 24 hpi and decapped with mRNA decapping enzyme (NEB# M0608S) and ligated head to tail by using T4 RNA ligase 1 ...
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bioRxiv - Immunology 2020Quote: ... CD45 catalytic domain DNA constructs were transformed into the BL21 (DE3) strain (NEB) Escherichia coli and plated on LB agar with ampicillin (100 mg/ml) ...
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bioRxiv - Genomics 2023Quote: ... Second strand synthesis was performed after thawing by the addition of 5 µL of second strand synthesis mix (3 µL of elution buffer [Qiagen], 1.33 µL mRNA second strand synthesis buffer and 0.66 µL of second strand synthesis enzyme mix [NEB]) and incubated at 16°C for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Editing activity of α-synCas was evaluated using EnGen Mutation Detection Kit (NEB). Briefly ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and about 200 ng of DNA was digested with the type II b enzyme BcgI (New England Biolabs). This enzyme cuts both upstream and downstream of the 6 bp recognition site ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... 1 μM Ultramer was annealed with 1 μM T7-3G oligonucleotide in 1x Taq Buffer (NEB) in a final volume of 10 μl by heating the reaction up to 95°C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... a fragment including the SARS-CoV-2 RdRp catalytic residue was subcloned into pUC19 (NEB) and the resulting pUC19-RdRp was mutated at the RdRp catalytic residue by inverse PCR using mutation-introducing primers (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... 13.5 μL digestion reaction product was combined with 1.5 μL second-strand synthesis (SSS) buffer and 1 μL of SSS enzyme mix from the NEBNext mRNA Second Strand Synthesis Module (NEB) and incubated at 16 °C for 2.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... Second strand cDNA synthesis was performed with 1X SSS buffer and SSS enzyme (NebNext mRNA second strand synthesis kit NEB; thermocycler program ...
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bioRxiv - Immunology 2022Quote: ... Resulting mRNA products were capped with a 5’-Cap 1 structure using vaccinia capping enzyme (New England Biolabs, Ipswitch, MA) and Vaccinia 2’ O-methyltransferase (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The editing region was amplified with PCR using Q5 High-Fidelity 2X Master Mix (NEB) followed by purification the PCR product using the QIAquick PCR Purification Kit (QIAGEN) ...
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bioRxiv - Immunology 2022Quote: ... The spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described7,8.
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bioRxiv - Molecular Biology 2020Quote: ... 2 μL of 10x SSS buffer and 1 μL of SSS Enzyme (NEBNext mRNA Second Strand Synthesis Module, New England Biolabs, USA) were added to the 17 μL of the purified sample ...
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bioRxiv - Developmental Biology 2023Quote: Plasmids (otxG:Tet-ON 3G; TRE3Gs:Cas9; TRE3Gs:Kaede; U6:sg2; Table S1) were built by Gibson cloning (NEB, Cat# E2611) following standard protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Editing efficiency of bulk cell pools was initially confirmed by T7E1 (T7 Endonuclease 1, #M0302S, NEB) assay ...
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bioRxiv - Molecular Biology 2020Quote: ... 13.5 μl digestion reaction product was combined with 1.5 μl second-strand synthesis (SSS) buffer and 1 μl of SSS enzyme mix from the NEBNext mRNA Second Strand Synthesis Module (NEB, Cat #E6111) and incubated at 16 °C for 2.5 h ...
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bioRxiv - Immunology 2022Quote: ... Delta and BA.2 spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described10.
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bioRxiv - Molecular Biology 2024Quote: Restriction enzymes: all restriction enzymes acquired from NEB: BbsI (R0539S) ...
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bioRxiv - Microbiology 2020Quote: ... Gene editing donor vectors were assembled in PUC19 using Gibson assembly according to the manufacturer’s protocol (NEB), using PCR products amplified using KAPA HiFi HotStart ReadyMixPCR Kit (KAPABiosystems ...
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bioRxiv - Genetics 2020Quote: ... mRNA was prepared from total RNA by magnetic mRNA purification (Magnetic mRNA Isolation Kit,NEB) according to the manufacturer’s’ instructions with the following deviation ...
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bioRxiv - Microbiology 2021Quote: ... catalytic MTase mutant of AMt2 (AMt2-C78G) was generated via site-directed mutagenesis (NEB, Q5 Site-Directed Mutagenesis Kit), using primers listed in the primer table (Table S1) ...
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bioRxiv - Plant Biology 2020Quote: ... restriction enzyme (NEB), which only digests the wild-type MSL10 gene ...
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bioRxiv - Microbiology 2019Quote: ... Restriction enzymes (NEB), T4 DNA ligase (Promega) ...